European Journal of Biology

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DOI :10.26650/EurJBiol.2023.1372233   IUP :10.26650/EurJBiol.2023.1372233    Tam Metin (PDF)

Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast

Çağatay Tarhan, Sümeyra Zeynep Çalıcı, Buse Özden

Objective: Metformin, a well-known anti-diabetic drug and a caloric restriction mimetic, seems to attenuate aging through myriad cellular processes, wherein most of its mode of action is still elusive. Thus, bioinformatic analyses that might direct experimental studies are crucial. Moreover, uncharacterised proteins with unknown molecular functions might withhold information regarding metformin’s mode of action. Here, we aimed to elucidate genes encoding uncharacterised proteins that are somehow involved in metformin metabolism and elaborate their involvement through functional annotation to reveal novel cellular processes in which metformin interferes.

Materials and Methods: Total RNA isolation was conducted from Schizosaccharomyces pombe wild-type cells that were grown in standard and overnutrition conditions. Following the gene expression analysis of the uncharacterised proteins, the bioinformatics analysis of the up- and down-regulated uncharacterised proteins upon metformin treatment in both was conducted using the functional annotator called PANNZER2.

Results: Genes that might be related to cellular processes such as meiosis, protein folding, calcium homeostasis, and heme production are up- and down-regulated upon metformin treatment. Moreover, the up-regulation of apoptosis and antioxidationrelated genes and the down-regulation of mitosis, DNA damage, apoptosis, mitochondria, and telomere-capping-related genes were also determined.

Conclusion: We effectively identified associations between metformin and a wide range of cellular processes and genetic mechanisms through the comprehensive annotation of uncharacterised genes. Our findings are consistent with the literature, and many of these uncharacterised proteins could be used as targets for research into aging in the future.

Anahtar Kelimeler: Metformin, Aging, Uncharacterized Proteins, Schizosaccharomyces pombe

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APA

Tarhan, Ç., Çalıcı, S.Z., & Özden, B. (2023). Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast. European Journal of Biology, 82(2), 196-211. https://doi.org/10.26650/EurJBiol.2023.1372233


AMA

Tarhan Ç, Çalıcı S Z, Özden B. Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast. European Journal of Biology. 2023;82(2):196-211. https://doi.org/10.26650/EurJBiol.2023.1372233


ABNT

Tarhan, Ç.; Çalıcı, S.Z.; Özden, B. Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 196-211, 2023.


Chicago: Author-Date Style

Tarhan, Çağatay, and Sümeyra Zeynep Çalıcı and Buse Özden. 2023. “Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast.” European Journal of Biology 82, no. 2: 196-211. https://doi.org/10.26650/EurJBiol.2023.1372233


Chicago: Humanities Style

Tarhan, Çağatay, and Sümeyra Zeynep Çalıcı and Buse Özden. “Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast.” European Journal of Biology 82, no. 2 (May. 2024): 196-211. https://doi.org/10.26650/EurJBiol.2023.1372233


Harvard: Australian Style

Tarhan, Ç & Çalıcı, SZ & Özden, B 2023, 'Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast', European Journal of Biology, vol. 82, no. 2, pp. 196-211, viewed 2 May. 2024, https://doi.org/10.26650/EurJBiol.2023.1372233


Harvard: Author-Date Style

Tarhan, Ç. and Çalıcı, S.Z. and Özden, B. (2023) ‘Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast’, European Journal of Biology, 82(2), pp. 196-211. https://doi.org/10.26650/EurJBiol.2023.1372233 (2 May. 2024).


MLA

Tarhan, Çağatay, and Sümeyra Zeynep Çalıcı and Buse Özden. “Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 196-211. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1372233


Vancouver

Tarhan Ç, Çalıcı SZ, Özden B. Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast. European Journal of Biology [Internet]. 2 May. 2024 [cited 2 May. 2024];82(2):196-211. Available from: https://doi.org/10.26650/EurJBiol.2023.1372233 doi: 10.26650/EurJBiol.2023.1372233


ISNAD

Tarhan, Çağatay - Çalıcı, SümeyraZeynep - Özden, Buse. “Functional Annotation of Uncharacterised Proteins Whose Expression Patterns Affect the Lifespan under Metformin Treatment in Fission Yeast”. European Journal of Biology 82/2 (May. 2024): 196-211. https://doi.org/10.26650/EurJBiol.2023.1372233



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Gönderim08.10.2023
Kabul19.11.2023
Çevrimiçi Yayınlanma13.12.2023

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