Araştırma Makalesi


DOI :10.26650/EurJBiol.2024.1393295   IUP :10.26650/EurJBiol.2024.1393295    Tam Metin (PDF)

Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells

Zeynep DemirAysun ÖzkanEsin Arı

Objective: This study aims to determine the effects of the Gypsophila bicolor Grossh. root extract on cell viability and to investigate its protective (antioxidant) effects against hydrogen peroxide (H2O2)-induced cytotoxicity and membrane damage in A431, A549, and H1299 cells.

Materials and Methods: The study uses the Cell Titer-BlueR Cell Viability assay to determine the cytotoxic effects of catechol on cells and the fluorometric method to determine cellular malondialdehyde (MDA) levels. The study uses DPPH free radical scavenging, superoxide anion scavenging, hydroxyl radical scavenging, and reducing power assays to determine the antioxidant capacity of the G. bicolor root extract.

Results: The IC50 values were respectively calculated as 60, 200, and 70 μg/mL in the H1299, A549, and A431 cells incubated with the root extract for 24 h. The IC50 values of H2O2, a strong oxidizing agent, were found to be 50, 400, and 295 μM, respectively. The most effective cytoprotective concentrations against H2O2 cytotoxicity in the cells pre-incubated with low concentrations of root extract were found to be 5 μg/mL for the A549 and A431 cells and 10 μg/mL for the H1299 cells. MDA levels increased in cells exposed to H2O2 and the root extract (IC50 and IC70) but decreased in the cells pre-incubated with low doses of root extract prior to H2O2 exposure. The root extract’s antioxidant capacity has also been supported by other tests.

Conclusion: While the root extract caused membrane damage in cells due to high concentrations, it showed a protective effect against H2O2 at low concentrations.


PDF Görünüm

Referanslar

  • Liu WJ, Du Y, Wen R, Yang M, Xu J. Drug resistance to targeted therapeutic strategies in non-small cell lung cancer. Pharmacol Ther. 2020;206:107438. doi:10.1016/j.pharmthera.2019.107438 google scholar
  • Zhang L, Man S, Wang Y, et al. Paris Saponin II induced apoptosis via activation of autophagy in human lung cancer cells. Chem Biol Interact. 2016;253:125-133. google scholar
  • Liu J, Xu Y, Yang J, et al. Discovery, semisynthesis, biological activities, and metabolism of ocotillol-type saponins. J Ginseng Res. 2017;41(3):373-378. google scholar
  • Zhang W, Luo JG, Zhang C, Kong LY. Different apoptotic effects of triterpenoid saponin-rich Gypsophila oldhamiana root extract on human hepatoma SMMC-7721 and normal human hepatic L02 cells. Biol Pharm Bull. 2013;36(7):1080-1087. google scholar
  • Karakas FP, Turker AU, Karakas A, Mshvildadze V, Pichette A, Legault J. In vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy—A medicinal plant. J Herbal Med. 2017;8:31-39. google scholar
  • Shin D, Moon HW, Oh Y, Kim K, Kim DD, Lim CJ. Defen-sive properties of ginsenoside re against UV-B-induced oxidative stress through up-regulating glutathione and superoxide dismu-tase in HaCaT keratinocytes. Iran J Pharm Res. 2018;17(1):249-260. google scholar
  • Vukic MD, Vukovic NL, Djelic GT, et al. Phytochemical analysis, antioxidant, antibacterial and cytotoxic activity of different plant organs of Eryngium serbicum L. Ind Crop Prod. 2018;115:88-97. google scholar
  • Güçlü Üstündağ Ö, Mazza G. Effects of pressurized low polarity water extraction parameters on antioxidant properties and com-position of cow cockle seed extracts. Plant Foods Hum Nutr. 2008;64:32-38. google scholar
  • Cam I, Topuz A. Production of soapwort concentrate and soapwort powder and their use in Turkish delight and tahini halvah. J Food Process Eng. 2017;41. doi:10.1111/jfpe.12605 google scholar
  • Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000;14(5):323-328. google scholar
  • Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: A simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987;165(1):215-219. google scholar
  • Oyaizu M. Studies on products of browning reaction antioxida-tive activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet. 1986;44(6):307-315. google scholar
  • Liu Q, Zhu G, Huang P. Anti-inflammatory, analgesic and sedative effects of Leontice kiangnanensis P.L. Chiu. Zhongguo Zhong Yao Za Zhi. 1991;16(1):50-65. google scholar
  • Gloeckner H, Jonuleit T, Lemke HD. Monitoring of cell viability and cell growth in a hollow-fiber bioreactor by use of the dye Alamar Blue. J Immunol Methods. 2001;252(1-2):131-138. google scholar
  • Wasowicz W, Neve J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: Importance of extraction pH and influence of sample preservation and storage. Clin Chem. 1993;39(12):2522-2526. google scholar
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254. google scholar
  • Ozdamar K. Statistical Data Analysis with Packaged Programs. google scholar
  • Arslan I, Celik A. Saponin rich fractions (SRPs) from soap-wort show antioxidant and hemolytic activity. APCBEE Procedia. 2013;7:103-108. google scholar
  • Hamdi A, Jaramillo-Carmona S, Srairi Beji R, et al. The phy-tochemical and bioactivity profiles of wild Asparagus albus L. plant. Food Res Int. 2017;99(Pt1):720-729. google scholar
  • Ma JS, Liu H, Han CR, et al. Extraction, charac-terization and antioxidant activity of polysaccharide from Pouteria campechiana seed. Carbohydr Polym. 2020;229:115409. doi:10.1016/j.carbpol.2019.115409 google scholar
  • Zhou Y, Ma W, Wang L, et al. Characterization and antioxidant activity of the oligo-maltose frac-tion from Polygonum cillinerve. Carbohydrate Polymers. 2019;226:115307. doi:10.1016/j.carbpol.2019.115307 google scholar
  • Singh R, Sharma S, Sharma V. Comparative and quanti-tative analysis of antioxidant and scavenging potential of Indigofera tinctoria Linn. extracts. J Integr Med. 2015;13(4):269-278. google scholar
  • Tian X, Feng J, Tang H, et al. New cytotoxic triterpenoid saponins from the whole plant of Clematis lasiandra Maxim. Fitoterapia. 2013;90:233-239. google scholar
  • Zhao M, Ma N, Qiu F, et al. Triterpenoid saponins from the roots of Clematis argentilucida and their cytotoxic activity. Planta Med. 2014;80(11):942-948. google scholar
  • Tilkat EA, Batibay H, Yener I, et al. Determination of enzyme inhibition potential and anticancer effects of Pistacia khinjuk stocks raised in in vitro and in vivo conditions. Agronomy. 2021;11(1):154. doi:10.3390/agronomy11010154 google scholar
  • Luo H, Huang J, Liao WG, Huang QY, Gao YQ. The antioxidant effects of garlic saponins protect PC12 cells from hypoxia-induced damage. Br J Nutr. 2011;105(8):1164-1172. google scholar
  • Saeedi Rad A, Esmaeili S, Mohammadi Motamed S, Hamzeloo-Moghadam M. Cytotoxicity of two gypsophila species to hu-man breast adenocarcinoma (MCF-7). Internl Pharm Acta (IPA). 2018;1(1):82-82. google scholar
  • Kaplan, A. GC-MS profiling, antioxidant, antimicrobial activities, DNA cleavage effect of Symphytum aintabicum Hub.-Mor. & Wickens (Boraginaceae) and its anticancer activityon MCF-7 cell line. Turkish J Bot. 2021;45(8):750-764. google scholar
  • Xu X-Y, Wang Z, Ren S, et al. Improved protective effects of Amer-ican ginseng berry against acetaminophen-induced liver toxic-ity through TNF-a-mediated caspase-3/-8/-9 signaling pathways. Phytomedicine. 2018;51:128-138. google scholar
  • Wang Y, Che J, Zhao H, Tang J, Shi G. Platycodin D inhibits oxidative stress and apoptosis in H9c2 cardiomyocytes follow-ing hypoxia/reoxygenation injury. Biochem Biophys Res Commun. 2018;503(4):3219-3224. google scholar

Atıflar

Biçimlendirilmiş bir atıfı kopyalayıp yapıştırın veya seçtiğiniz biçimde dışa aktarmak için seçeneklerden birini kullanın


DIŞA AKTAR



APA

Demir, Z., Özkan, A., & Arı, E. (2024). Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells. European Journal of Biology, 83(2), 123-130. https://doi.org/10.26650/EurJBiol.2024.1393295


AMA

Demir Z, Özkan A, Arı E. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells. European Journal of Biology. 2024;83(2):123-130. https://doi.org/10.26650/EurJBiol.2024.1393295


ABNT

Demir, Z.; Özkan, A.; Arı, E. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells. European Journal of Biology, [Publisher Location], v. 83, n. 2, p. 123-130, 2024.


Chicago: Author-Date Style

Demir, Zeynep, and Aysun Özkan and Esin Arı. 2024. “Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells.” European Journal of Biology 83, no. 2: 123-130. https://doi.org/10.26650/EurJBiol.2024.1393295


Chicago: Humanities Style

Demir, Zeynep, and Aysun Özkan and Esin Arı. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells.” European Journal of Biology 83, no. 2 (Dec. 2024): 123-130. https://doi.org/10.26650/EurJBiol.2024.1393295


Harvard: Australian Style

Demir, Z & Özkan, A & Arı, E 2024, 'Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells', European Journal of Biology, vol. 83, no. 2, pp. 123-130, viewed 22 Dec. 2024, https://doi.org/10.26650/EurJBiol.2024.1393295


Harvard: Author-Date Style

Demir, Z. and Özkan, A. and Arı, E. (2024) ‘Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells’, European Journal of Biology, 83(2), pp. 123-130. https://doi.org/10.26650/EurJBiol.2024.1393295 (22 Dec. 2024).


MLA

Demir, Zeynep, and Aysun Özkan and Esin Arı. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells.” European Journal of Biology, vol. 83, no. 2, 2024, pp. 123-130. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1393295


Vancouver

Demir Z, Özkan A, Arı E. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells. European Journal of Biology [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];83(2):123-130. Available from: https://doi.org/10.26650/EurJBiol.2024.1393295 doi: 10.26650/EurJBiol.2024.1393295


ISNAD

Demir, Zeynep - Özkan, Aysun - Arı, Esin. Membrane Damaging and Protective Effects of Gypsophila bicolor’s Grossh. (Caryophyllaceae) Root Extract on H1299, A549, and A431 Cells”. European Journal of Biology 83/2 (Dec. 2024): 123-130. https://doi.org/10.26650/EurJBiol.2024.1393295



ZAMAN ÇİZELGESİ


Gönderim20.11.2023
Kabul11.02.2024
Çevrimiçi Yayınlanma14.08.2024

LİSANS


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


PAYLAŞ




İstanbul Üniversitesi Yayınları, uluslararası yayıncılık standartları ve etiğine uygun olarak, yüksek kalitede bilimsel dergi ve kitapların yayınlanmasıyla giderek artan bilimsel bilginin yayılmasına katkıda bulunmayı amaçlamaktadır. İstanbul Üniversitesi Yayınları açık erişimli, ticari olmayan, bilimsel yayıncılığı takip etmektedir.