European Journal of Biology

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DOI :10.26650/EurJBiol.2023.1279151   IUP :10.26650/EurJBiol.2023.1279151    Tam Metin (PDF)

Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding

Yüksel Gezgin, Gülce Güralp, Ayşe Berçin Barlas, Rengin Eltem

Objective: Trichoderma genus are environmentally friendly, targeted biocontrol agents used in organic agriculture. Currently, due to the increasing number of organic farming practices, Trichoderma species form a good market as commercial biocontrol agents. This study aims to make morphological and molecular identification of Trichoderma isolates, which were found to be potential biocontrol agents against plant pathogenic fungi, and to perform phylogenetic diversity analyses of these species using different bioinformatics tools.

Materials and Methods: Two different gene regions (the nuclear ribosomal internal transcribed spacer (ITS) and translation elongation factor 1 (EF) were used for molecular identification of Trichoderma isolates in this study. Polymerase Chain Reaction (PCR) related regions were amplified and sequenced using primers specific to these gene regions. Following molecular identifications based on these two different gene regions, phylogenetic trees were drawn and polymorphic regions in the nucleotide sequences of these genes were determined.

Results: As a result of the study, Trichoderma isolates were determined as T. citrinoviride Bissett and T. atroviride P. Karst. at the species level. This study not only provides essential information about the biodiversity of Trichoderma species, which is a biocontrol agent, but also allows the design of new species-specific primers based on the polymorphic regions of both species.

Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.

Anahtar Kelimeler: T. citrinoviride, T. atroviride, BLAST, TrichOKEY, TrichoBLAST, targeted biocontrol agents, DNA barcoding

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APA

Gezgin, Y., Güralp, G., Barlas, A.B., & Eltem, R. (2023). Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology, 82(1), 59-69. https://doi.org/10.26650/EurJBiol.2023.1279151


AMA

Gezgin Y, Güralp G, Barlas A B, Eltem R. Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology. 2023;82(1):59-69. https://doi.org/10.26650/EurJBiol.2023.1279151


ABNT

Gezgin, Y.; Güralp, G.; Barlas, A.B.; Eltem, R. Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology, [Publisher Location], v. 82, n. 1, p. 59-69, 2023.


Chicago: Author-Date Style

Gezgin, Yüksel, and Gülce Güralp and Ayşe Berçin Barlas and Rengin Eltem. 2023. “Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding.” European Journal of Biology 82, no. 1: 59-69. https://doi.org/10.26650/EurJBiol.2023.1279151


Chicago: Humanities Style

Gezgin, Yüksel, and Gülce Güralp and Ayşe Berçin Barlas and Rengin Eltem. “Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding.” European Journal of Biology 82, no. 1 (May. 2024): 59-69. https://doi.org/10.26650/EurJBiol.2023.1279151


Harvard: Australian Style

Gezgin, Y & Güralp, G & Barlas, AB & Eltem, R 2023, 'Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding', European Journal of Biology, vol. 82, no. 1, pp. 59-69, viewed 19 May. 2024, https://doi.org/10.26650/EurJBiol.2023.1279151


Harvard: Author-Date Style

Gezgin, Y. and Güralp, G. and Barlas, A.B. and Eltem, R. (2023) ‘Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding’, European Journal of Biology, 82(1), pp. 59-69. https://doi.org/10.26650/EurJBiol.2023.1279151 (19 May. 2024).


MLA

Gezgin, Yüksel, and Gülce Güralp and Ayşe Berçin Barlas and Rengin Eltem. “Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding.” European Journal of Biology, vol. 82, no. 1, 2023, pp. 59-69. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1279151


Vancouver

Gezgin Y, Güralp G, Barlas AB, Eltem R. Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology [Internet]. 19 May. 2024 [cited 19 May. 2024];82(1):59-69. Available from: https://doi.org/10.26650/EurJBiol.2023.1279151 doi: 10.26650/EurJBiol.2023.1279151


ISNAD

Gezgin, Yüksel - Güralp, Gülce - Barlas, AyşeBerçin - Eltem, Rengin. “Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding”. European Journal of Biology 82/1 (May. 2024): 59-69. https://doi.org/10.26650/EurJBiol.2023.1279151



ZAMAN ÇİZELGESİ


Gönderim07.04.2023
Kabul05.06.2023
Çevrimiçi Yayınlanma26.06.2023

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