Araştırma Makalesi


DOI :10.26650/EurJBiol.2018.400108   IUP :10.26650/EurJBiol.2018.400108    Tam Metin (PDF)

Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet

Debora MorettiWiolene NordiRaul Machado Neto

Objective: The electrophoretic profile of serum proteins was investigated in juvenile Piaractus mesopotamicus and Salminus brasiliensis fed with diets containing bovine colostrum, a nutraceutical food, in lyophilized form (LBC) for either 30 or 60 days. Materials and Methods: Blood samples were collected from juveniles of P. mesopotamicus and S. brasiliensis fed for either 30 or 60 days with diets containing 0%, 10% or 20% of LBC. Serum protein fractions were then determined by electrophoresis in agarose gel. Results: The P. mesopotamicus, an omnivorous fish, showed six serum protein fractions, while the S. brasiliensis, a carnivorous fish, showed four serum protein fractions. In both species the albumin fraction showed higher protein content at 30 days than at 60 days (p<0.05), indicating an inverse relationship with growth. The 5th and 4th fraction in mobility from the P. mesopotamicus and S. brasiliensis serum, respectively, were positioned in a gamma-globulin zone. In P. mesopotamicus, the concentration of protein in the gamma-globulin zone was higher at 60 days than at 30 days (p<0.05). In S. brasiliensis, in turn, the concentration of protein in the gamma-globulin zone was higher at 30 days than at 60 days (p<0.05). In juvenile P. mesopotamicus, a higher concentration of protein was also observed in the gamma-globulin zone in the 0% LBC compared to 10% and 20% LBC (p<0.05). Thus, the feeding period influenced only the fractions that were positioned in the albumin and gamma-globulin migration zone in both species. Conclusion: The consumption of bovine colostrum decreased the concentration of protein in gamma-globulin fraction of the P. mesopotamicus, indicating that these juveniles had either less stimulus for their own synthesis of defense elements or an immunosuppressive effect of bovine colostrum ingestion.

PDF Görünüm

Referanslar

  • 1. Venturieri RL. Pesque-pague no Estado de São Paulo: Vetor de desenvolvimento e opção de turismo e lazer. 1st ed. São Paulo, SP, BR: ECO Associação para Estudos do Ambiente; 2003. google scholar
  • 2. Urbinati EC, Gonçalves FD. Pacu (Piaractus mesopotamicus). In: Baldisseroto B, Gomes LC, editors Espécies nativas para piscicultura no Brasil. Santa Maria, RGS, BR: UFSM; 2005. pp 225-246. google scholar
  • 3. Esteves KE, Sant´anna CL. Pesqueiros sob uma visão integrada de meio ambiente, Saúde Pública e Manejo: Um estudo da região metropolitana de São Paulo. São Carlos, SP, BR: Editora Rima; 2006. google scholar
  • 4. Doherty NS, Littman BH, Reilly K, Swindell AC, Buss JM Anderson NL. Analysis of changes in acute-phase plasma proteins in an acute inflammatory response and in rheumatoid arthritis using two-dimensional gel electrophoresis. Electrophoresis 1998; 19: 355-363. google scholar
  • 5. Cray C, Besselsen DG, Hart JL, Yoon D, Rodriguez M, Zaias J, Altman NH. Quantitation of acute phase proteins and protein electrophoresis in monitoring the acute inflammatory process in experimentally and naturally infected mice. Comp Med 2010; 60: 263-271. google scholar
  • 6. Kekic M, dos Remedios CG. Electrophoretic monitoring of pollutants: effect of cations and organic compounds on protein interactions monitored by native gel electrophoresis. Electrophoresis 1999; 20: 2053–2058. google scholar
  • 7. Sharaf-Eldeen Kh, Abdel-Hamid NA. Sublethal effects of copper sulphate, malathion and paraquat on protein pattern of Oreochromis niloticus. Egypt J Aquat Biol Fish 2002; 6: 167–182. google scholar
  • 8. Osman AGM, Al-Awadhi RM, Harabawy ASA, Mahmoud UM. Evaluation of the use of protein electrophoresis of the African catfish Clarias gariepinus (Burchell, 1822) for biomonitoring aquatic pollution. Environ Res J 2010; 4: 235–243. google scholar
  • 9. Fazio F, Marafioti S, Torre A, Sanfilippo M, Panzera M, Faggio C. Haematological and serum protein profiles of Mugil cephalus: effect of two different habitats. Ichthyol Res 2013; 60: 36-42. google scholar
  • 10. Blum JW, Hammon H. Colostrum effects on the gastrointestinal tract, and on nutritional, endocrine and metabolic parameters in neonatal calves. Liv Prod Sci 2000; 66: 151-159. google scholar
  • 11. Blum JW, Baumrucker CR. Colostral and milk insulin-like growth factors and related substances: mammary gland and neonatal (intestinal and systemic) targets. Dom Anim Endocrinol 2002; 23: 101-110. google scholar
  • 12. Daniele C, Machado-Neto R, Baracat RS, Bessi R. Effect of different feeding programs employing supplemental colostrum on serum total protein and albumin, and performance of newborn calves. Sci Agric 1994; 51: 381-388. google scholar
  • 13. Nocek JE, Braund DG, Warner RG. Influence of neonatal colostrum administration, immunoglobulin, and continued feeding of colostrum on calf gain, health and serum protein. J Dairy Sci 1984; 67: 319-333. google scholar
  • 14. Baracat RS, Machado Neto R, Daniele C, Bessi R, Packer IU. Extended colostrum feeding and passive immunity in newborn calves. Pesq Agrop Bras 1997; 32: 215-220. google scholar
  • 15. Association of Oficial Analytical Chemists. Oficial methods of analyses. Washington, DC, USA: Association of Oficial Analytical Chemists; 1990. google scholar
  • 16. Reinhold JG. Total protein, albumin and globulin. In: Reiner M, editor. Standard Methods of Clinical Chemistry. New York, NY, USA: Academic Press; 1953. pp. 88. google scholar
  • 17. Badawi HK. Electrophoretic studies of serum proteins of four Tilapia species (Pisces) Marine Biology 1971; 8: 96-98. google scholar
  • 18. Atamanalp M, Yanik T, Haliloglu HI, Sitki AM. Alterations in the haematological parameters of rainbow trout, Oncorhynchus mykiss, exposed to cypermethrin. Israeli J Aquaculture 2002; 54: 99–103. google scholar
  • 19. Maceda-Veiga A, Monroya M, Viscorb G, De Sostoa A. Changes in non-specific biomarkers in the Mediterranean barbell (Barbus meridionalis) exposed to sewage effluents in a Mediterranean stream (Catalonia, NE Spain). Aquat Toxicol 2010; 100: 229–237. google scholar
  • 20. Scapigliati G, Scalia D, Marras A, Meloni S, Mazzini M. Immunoglobulin levels in the teleost sea bass Dicentrarchus labrax (L.) in relation to age, season, and water oxygenation. Aquaculture 1999; 174: 207-212. google scholar
  • 21. Boudry C, Thewis A. Bovine colostrum as a natural growth factor for newly-weaned piglets: a review. Bull UASVM Anim Sci Biotechnol 2009; 66: 1-2. google scholar
  • 22. Menezes MR, Qasim SZ. Use of electrophoresis and immunoelectrophoresis in taxonomic and pollution studies. Proc Indian Acad Sci (Anim Sci) 1984; 93: 179-198. google scholar
  • 23. El-Serafy SS, Abdel-Hamide NAH, Awwad MH, Azab MS. Comparative study on electrophoretic protein pattern characterization of Tilapia species in the river nile, Egypt. Egypt J AquaL BioL & Fish 2006; 10: 147-178. google scholar
  • 24. Yilmaz M, Yilmaz HR, Alas A. An electrophoretic taxonomic study on sérum proteins of Acanthobrama marmid, Leuciscus cephalus, and Chondrostoma regium. EuroAsia J BioSci 2007; 3: 22-27. google scholar
  • 25. Shagufta SSJK, Gayasuddin M. Comparative study of serum proteins of man and four Teleosts: using polyacrylamide gel electrophoresis. Adv Biol Res 2011; 5: 170-173. google scholar
  • 26. Akinwande AA, Fagbenro OA, Adebayo OT. Serum protein pattern in interspecific and intergeneric hybrids of Heterobranchus longiflis, Clarias gariepinus and Clarias anguillaris in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Int J Fish Aquac 2012; 4: 202-208. google scholar
  • 27. Gicking JC, Foley AM, Harr KE, Raskin RE, Jacobson E. Plasma protein electrophoresis of the Atlantic Loggerhead sea turtle, Caretta caretta. J Herpetol Med Surg 2004; 14: 13-18. google scholar

Atıflar

Biçimlendirilmiş bir atıfı kopyalayıp yapıştırın veya seçtiğiniz biçimde dışa aktarmak için seçeneklerden birini kullanın


DIŞA AKTAR



APA

Moretti, D., Nordi, W., & Machado Neto, R. (2018). Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet. European Journal of Biology, 77(2), 59-64. https://doi.org/10.26650/EurJBiol.2018.400108


AMA

Moretti D, Nordi W, Machado Neto R. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet. European Journal of Biology. 2018;77(2):59-64. https://doi.org/10.26650/EurJBiol.2018.400108


ABNT

Moretti, D.; Nordi, W.; Machado Neto, R. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet. European Journal of Biology, [Publisher Location], v. 77, n. 2, p. 59-64, 2018.


Chicago: Author-Date Style

Moretti, Debora, and Wiolene Nordi and Raul Machado Neto. 2018. “Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet.” European Journal of Biology 77, no. 2: 59-64. https://doi.org/10.26650/EurJBiol.2018.400108


Chicago: Humanities Style

Moretti, Debora, and Wiolene Nordi and Raul Machado Neto. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet.” European Journal of Biology 77, no. 2 (Sep. 2025): 59-64. https://doi.org/10.26650/EurJBiol.2018.400108


Harvard: Australian Style

Moretti, D & Nordi, W & Machado Neto, R 2018, 'Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet', European Journal of Biology, vol. 77, no. 2, pp. 59-64, viewed 20 Sep. 2025, https://doi.org/10.26650/EurJBiol.2018.400108


Harvard: Author-Date Style

Moretti, D. and Nordi, W. and Machado Neto, R. (2018) ‘Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet’, European Journal of Biology, 77(2), pp. 59-64. https://doi.org/10.26650/EurJBiol.2018.400108 (20 Sep. 2025).


MLA

Moretti, Debora, and Wiolene Nordi and Raul Machado Neto. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet.” European Journal of Biology, vol. 77, no. 2, 2018, pp. 59-64. [Database Container], https://doi.org/10.26650/EurJBiol.2018.400108


Vancouver

Moretti D, Nordi W, Machado Neto R. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet. European Journal of Biology [Internet]. 20 Sep. 2025 [cited 20 Sep. 2025];77(2):59-64. Available from: https://doi.org/10.26650/EurJBiol.2018.400108 doi: 10.26650/EurJBiol.2018.400108


ISNAD

Moretti, Debora - Nordi, Wiolene - Machado Neto, Raul. Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet”. European Journal of Biology 77/2 (Sep. 2025): 59-64. https://doi.org/10.26650/EurJBiol.2018.400108



ZAMAN ÇİZELGESİ


Gönderim01.03.2018
Kabul19.06.2018
Çevrimiçi Yayınlanma02.12.2018

LİSANS


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


PAYLAŞ



İstanbul Üniversitesi Yayınları, uluslararası yayıncılık standartları ve etiğine uygun olarak, yüksek kalitede bilimsel dergi ve kitapların yayınlanmasıyla giderek artan bilimsel bilginin yayılmasına katkıda bulunmayı amaçlamaktadır. İstanbul Üniversitesi Yayınları açık erişimli, ticari olmayan, bilimsel yayıncılığı takip etmektedir.