European Journal of Biology

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DOI :10.26650/EurJBiol.2018.0016   IUP :10.26650/EurJBiol.2018.0016    Tam Metin (PDF)

The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells

Özlem Dağdeviren Özsöylemez, Gül Özcan

Objective: This study was conducted to determine the antiproliferative effects of paclitaxel (PAC) and Colchicum umbrosum plant extract on C-4 I and Vero cells. In addition, the apoptotic effects of plant extract on these cells were compared. Materials and Methods: C-4 I and Vero cells were treated with PAC and corm extract of C. umbrosum for 24 h. The antiproliferative effects of 0.01, 0.1, and 1 mg/mL of plant extract and 7.5, 15, and 30 nM of PAC and the half maximal inhibitory concentration (IC50) values were determined by the MTT method. Morphological changes occurring in the C-4 I and Vero cells were observed under phase-contrast and fluorescence microscopes using DAPI staining. Results: The IC50 values were found to be 0.01 mg/mL in C-4 I cells and 1 mg/mL in Vero cells for the plant extract and 15 nM in C-4 I and Vero cells for PAC. The apoptotic index (AI) values of the experimental groups of C-4 I and Vero cells were significantly increased compared to those of the control group (p<0.01) after treatment with IC50 concentration of the plant extract. The AI values of the plant extract treatment in C-4 I cells were higher than those in Vero cells at 24 and 48 h. Conclusion: Treatment with the IC50 concentration of the plant extract induced apoptotic cell death in C-4 I cells. It can worth be considered as a novel promising candidate for the treatment of cancer.
Anahtar Kelimeler: C-4 I, Vero, Colchicum umbrosum, paclitaxel, cancer

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Referanslar

  • 1. Johnstone RW, Ruefli AA, Lowe SW. Apoptosis: a link between cancer genetics and chemotherapy. Cell 2002; 108(2): 153-64. google scholar
  • 2. Catalano E, Maestri P, Cochis A, Azzimonti B, Varoni EM, Fraschini F, et al. Cytotoxic activity of a plant extract on cancer cells. Ital J Anat Embryol 2013; 118(2): 50. google scholar
  • 3. Demirgan R, Karagoz A, Pekmez M, Onay-Ucar E, Artun FT, Gurer C, et al. In vitro anticancer activity and cytotoxicity of some papaver alkaloids on cancer and normal cell lines. Afr J Tradit Complement Altern Med 2016; 13(3): 22-6. google scholar
  • 4. Peña-Morán OA, Villarreal ML, Álvarez-Berber L, Meneses-Acosta A, Rodríguez-López V. Cytotoxicity, post-treatment recovery, and selectivity analysis of naturally occurring podophyllotoxins from Bursera fagaroides var. fagaroides on breast cancer cell lines. Molecules 2016; 21(8): pii: E1013. google scholar
  • 5. Gupta A, Naraniwal M, Kothari V. Modern extraction methods for preparation of bioactive plant extracts. IJANS 2012; 1(1): 8-26. google scholar
  • 6. Korkina L and Kostyuk V. Biotechnologically produced secondary plant metabolites for cancer treatment and prevention. Curr Pharm Biotechno 2012; 13(1): 265-75. google scholar
  • 7. Ramasamy S, Wahab N.A, Abidin N.Z, Manickam S, Zakaria Z. Growth Inhibition of human gynecologic and colon cancer cells by Phyllanthus watsonii through apoptosis induction. PLoS One 2012; 7(4): e34793. google scholar
  • 8. Kma L. Roles of plant extracts and constituents in cervical cancer therapy. Asian Pac J Cancer Prev 2013; 14(6): 3429-36. google scholar
  • 9. Sgadari C, Toschi E, Palladino C, Barillari G, Carlei D, Cereseto A, et al. Mechanism of paclitaxel activity in Kaposi’s Sarcoma. J Immunol 2000; 165(1): 509-17. google scholar
  • 10. Jordan MA, Wendell K, Gardiner S, Derry WB, Copp H, Wilson L. Mitotic block induced in HeLa cells by low concentrations of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death. Cancer Res 1996; 56(4): 816-25. google scholar
  • 11. Combeau C, Provost J, Lancelin F, Tournoux Y, Prod’homme F, Herman F, et al. RPR112378 and RPR115781: two representatives of a new family of microtubule assembly inhibitors. Mol Pharmacol 2000; 57(3): 553-63. google scholar
  • 12. Dumontet C, Sikic BI. Mechanisms of action of and resistance to antitubulin agents: microtubule dynamics, drug transport, and cell death. J Clin Oncol 1999; 17(3): 1061-70. google scholar
  • 13. Pirildar S, Sutlupinar N, Atasever B, Erdem Kuruca S, Papouskova B, Śimánek V, Chemical constituents of the different parts of Colchicum baytopiorum and their cytotoxic activities on K562 and HL60 cell lines. Pharm Biol 2010; 48(1): 32-9. google scholar
  • 14. Jordan MA, Wilson L. Microtubules as a target for anticancer drugs. Nat Rev 2004; 4(4): 253-65. google scholar
  • 15. Piccolella M, Crippaa V, Messia E, Tetel MJ, Poletti A. Modulators of estrogen receptor inhibit proliferation and migration of prostate cancer cells. Pharmacol Res 2014; 79: 13-20. google scholar
  • 16. Wu CT, Lai JN, Tsai YT. The prescription pattern of Chinese herbal products that contain Dang-Qui and risk of endometrial cancer among Tamoxifen-treated female breast cancer survivors in Taiwan: A population-based study. PLoS One 2014, 9(12): e113887. google scholar
  • 17. Binkhorst L, Mathijssen R.H, Jager A, van Gelder T. Individualization of tamoxifen therapy: Much more than just CYP2D6 genotyping. Cancer Treat Rev 2015; 41(3): 289-99. google scholar
  • 18. Bisi A, Gobbi S, Merolle L, Farruggia G, Belluti F, Rampa A, Molnar J, et al. Design, synthesis and biological profile of new inhibitors of multidrug resistance associated proteins carrying a polycyclic scaffold. Eur J Med Chem 2015; 92: 471-80. google scholar
  • 19. Chauhan R, Verma N, Sharma SP, Bhargava R, Singh P. Role of apoptotic index, mitotic index and MIB-1 antibody expression as biomarkers in preneoplastic and neoplastic lesions of uterine cervix. Int J Res Med Sci 2016; 4(6): 2093-100. google scholar
  • 20. Artun FT, Karagoz A, Ozcan G, Melikoglu G, Anil S, Kultur S, et al. In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. Proceedings 2017; 1: 1019. google scholar
  • 21. Ozcan G, Dagdeviren Ozsoylemez O, Akman G, Khalilia W, Tezel B.Y, Karagoz A, Melikoglu G, Anil S, Kultur S, Sutlupinar N. Screening for antitumor activity of various plant extracts on HeLa and C 4-I cell lines. J BUON 2016; 21(6): 1552-60. google scholar
  • 22. Dağdeviren Ö. Acı Çiğdem (Colchicum umbrosum S. ve Colchicum baytopiorum C.D.) ekstrelerinin Hela hücreleri üzerine anti-tümöral etkilerinin araştırılması. İÜ Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi. 2010. google scholar
  • 23. Serbes U, Ozsoylemez OD, Ozcan G. Evaluation of paclitaxel effects in the pattern of expression of survival and apoptotic genes regulators in HeLa cells. Curr Pharm Biotechnol 2016; 17(12): 1058-67. google scholar
  • 24. Ozsoylemez OD, Ozturk M, Sutlupinar N, Kayacan S, Tuncdemir M, Ozan G. The effects of Colchicum baytopiorum on regulatory genes of apoptotic and autophagic cell death in HeLa cells. Curr Pharm Biotechnol 2016; 17(15): 1369-76. google scholar
  • 25. Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E, Lorberboum-Galski H. Evaluating medicinal plants for anticancer activity. ScientificWorldJournal 2014; 2014: 721402. google scholar
  • 26. Delebinski C, Georgi S, Kleinsimon S, Twardziok M, Kopp B, Melzig MF, et al. Analysis of proliferation and apoptotic induction by 20 steroid glycosides in 143B osteosarcoma cells in vitro. Cell Prolif 2015; 48(5): 600-10. google scholar
  • 27. Radogna F, Dicato M, Diederich M. Cancer-type-specific crosstalk between autophagy, necroptosis and apoptosis as a pharmacological target. Biochem Pharmacol 2015; 94(1): 1-11. google scholar
  • 28. Mirzayans R, Andrais B, Scott A, Murray D. New insights into p53 signaling and cancer cell response to DNA damage: implications for cancer therapy. Biomed Res Int 2012; 2012: 170325. google scholar
  • 29. Ganapathy S, Xiao S, Yang M, Qi M, Choi DE, Ha CS, et al. A low-dose arsenic-induced p53 protein-mediated metabolic mechanism of radiotherapy protection. J Biol Chem 2014; 289(8): 5340-7. google scholar
  • 30. Aubrey BJ, Kelly GL, Janic A, Herold MJ, Strasser A. How does p53 induce apoptosis and how does this relate to p53-mediated tumour supression? Cell Death Differ 2018; 25(1): 104-13. google scholar
  • 31. Gewies A. Introduction to Apoptosis. ApoReview 2003; 1-26. google scholar
  • 32. Goldstein JC, Kluck RM, Green DR. A single cell analysis of apoptosis: ordering the apoptotic phenotype. Ann N Y Acad Sci 2000; 926(1): 132-41. google scholar

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APA

Dağdeviren Özsöylemez, Ö., & Özcan, G. (2018). The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells. European Journal of Biology, 77(2), 81-88. https://doi.org/10.26650/EurJBiol.2018.0016


AMA

Dağdeviren Özsöylemez Ö, Özcan G. The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells. European Journal of Biology. 2018;77(2):81-88. https://doi.org/10.26650/EurJBiol.2018.0016


ABNT

Dağdeviren Özsöylemez, Ö.; Özcan, G. The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells. European Journal of Biology, [Publisher Location], v. 77, n. 2, p. 81-88, 2018.


Chicago: Author-Date Style

Dağdeviren Özsöylemez, Özlem, and Gül Özcan. 2018. “The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells.” European Journal of Biology 77, no. 2: 81-88. https://doi.org/10.26650/EurJBiol.2018.0016


Chicago: Humanities Style

Dağdeviren Özsöylemez, Özlem, and Gül Özcan. “The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells.” European Journal of Biology 77, no. 2 (Sep. 2025): 81-88. https://doi.org/10.26650/EurJBiol.2018.0016


Harvard: Australian Style

Dağdeviren Özsöylemez, Ö & Özcan, G 2018, 'The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells', European Journal of Biology, vol. 77, no. 2, pp. 81-88, viewed 20 Sep. 2025, https://doi.org/10.26650/EurJBiol.2018.0016


Harvard: Author-Date Style

Dağdeviren Özsöylemez, Ö. and Özcan, G. (2018) ‘The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells’, European Journal of Biology, 77(2), pp. 81-88. https://doi.org/10.26650/EurJBiol.2018.0016 (20 Sep. 2025).


MLA

Dağdeviren Özsöylemez, Özlem, and Gül Özcan. “The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells.” European Journal of Biology, vol. 77, no. 2, 2018, pp. 81-88. [Database Container], https://doi.org/10.26650/EurJBiol.2018.0016


Vancouver

Dağdeviren Özsöylemez Ö, Özcan G. The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells. European Journal of Biology [Internet]. 20 Sep. 2025 [cited 20 Sep. 2025];77(2):81-88. Available from: https://doi.org/10.26650/EurJBiol.2018.0016 doi: 10.26650/EurJBiol.2018.0016


ISNAD

Dağdeviren Özsöylemez, Özlem - Özcan, Gül. “The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells”. European Journal of Biology 77/2 (Sep. 2025): 81-88. https://doi.org/10.26650/EurJBiol.2018.0016



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