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DOI :10.26650/EuroJBiol.2018.0003   IUP :10.26650/EuroJBiol.2018.0003    Tam Metin (PDF)

The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells

Canan ErogluEbru AvciMucahit SecmeYavuz DodurgaHasibe Cingilli VuralErcan Kurar

Objective:Prostate cancer is the second most common cause of cancer-related deaths in men. Nowadays, new treatment approaches have been tested for cancer therapy including natural compounds with low toxicity. Ferulic acid (FA) is known as an abundant phenolic compound found in various fruits and vegetables. As a potent antioxidant, the anticarcinogenic effect of FA has been demonstrated in various cancer cell lines. The objective of this study was to investigate the combined effect of FA and gemcitabine on apoptosis and metastasis in PC-3 human prostate cancer cell lines.

Materials and Methods:Cell viability was determined using the XTT method after the cells were treated with gemcitabine or FA and gemcitabine. According to the results of cytotoxicity assays, PC-3 cells were treated with <IC50 doses of combination (200 μM FA and 35 μM gemcitabine) and IC50 dose of gemcitabine. Expressions of genes that are important in apoptosis and metastasis pathways were evaluated in dose and control groups by qPCR.

Results: According to the results, the combination of FA and gemcitabine affected the expression of more genes in apoptosis and metastasis with a higher fold change compared with the single treatment of gemcitabine in PC-3 human prostate cancer cell lines.

Conclusion: Our study indicates that FA can be an effective part of the combination treatments.

Anahtar Kelimeler: Ferulic acidgemcitabinePC-3 cell line

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DIŞA AKTAR



APA

Eroglu, C., Avci, E., Secme, M., Dodurga, Y., Cingilli Vural, H., & Kurar, E. (2018). The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells. European Journal of Biology, 77(1), 32-37. https://doi.org/10.26650/EuroJBiol.2018.0003


AMA

Eroglu C, Avci E, Secme M, Dodurga Y, Cingilli Vural H, Kurar E. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells. European Journal of Biology. 2018;77(1):32-37. https://doi.org/10.26650/EuroJBiol.2018.0003


ABNT

Eroglu, C.; Avci, E.; Secme, M.; Dodurga, Y.; Cingilli Vural, H.; Kurar, E. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells. European Journal of Biology, [Publisher Location], v. 77, n. 1, p. 32-37, 2018.


Chicago: Author-Date Style

Eroglu, Canan, and Ebru Avci and Mucahit Secme and Yavuz Dodurga and Hasibe Cingilli Vural and Ercan Kurar. 2018. “The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells.” European Journal of Biology 77, no. 1: 32-37. https://doi.org/10.26650/EuroJBiol.2018.0003


Chicago: Humanities Style

Eroglu, Canan, and Ebru Avci and Mucahit Secme and Yavuz Dodurga and Hasibe Cingilli Vural and Ercan Kurar. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells.” European Journal of Biology 77, no. 1 (Feb. 2024): 32-37. https://doi.org/10.26650/EuroJBiol.2018.0003


Harvard: Australian Style

Eroglu, C & Avci, E & Secme, M & Dodurga, Y & Cingilli Vural, H & Kurar, E 2018, 'The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells', European Journal of Biology, vol. 77, no. 1, pp. 32-37, viewed 24 Feb. 2024, https://doi.org/10.26650/EuroJBiol.2018.0003


Harvard: Author-Date Style

Eroglu, C. and Avci, E. and Secme, M. and Dodurga, Y. and Cingilli Vural, H. and Kurar, E. (2018) ‘The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells’, European Journal of Biology, 77(1), pp. 32-37. https://doi.org/10.26650/EuroJBiol.2018.0003 (24 Feb. 2024).


MLA

Eroglu, Canan, and Ebru Avci and Mucahit Secme and Yavuz Dodurga and Hasibe Cingilli Vural and Ercan Kurar. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells.” European Journal of Biology, vol. 77, no. 1, 2018, pp. 32-37. [Database Container], https://doi.org/10.26650/EuroJBiol.2018.0003


Vancouver

Eroglu C, Avci E, Secme M, Dodurga Y, Cingilli Vural H, Kurar E. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells. European Journal of Biology [Internet]. 24 Feb. 2024 [cited 24 Feb. 2024];77(1):32-37. Available from: https://doi.org/10.26650/EuroJBiol.2018.0003 doi: 10.26650/EuroJBiol.2018.0003


ISNAD

Eroglu, Canan - Avci, Ebru - Secme, Mucahit - Dodurga, Yavuz - Cingilli Vural, Hasibe - Kurar, Ercan. The Combination Effect of Ferulic Acid and Gemcitabine on Expression of Genes Related Apoptosis and Metastasis in PC-3 Prostate Cancer Cells”. European Journal of Biology 77/1 (Feb. 2024): 32-37. https://doi.org/10.26650/EuroJBiol.2018.0003



ZAMAN ÇİZELGESİ


Gönderim28.05.2018
Kabul04.06.2018
Çevrimiçi Yayınlanma22.06.2018

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