Sağlık Bilimlerinde İleri Araştırmalar Dergisi

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DOI :10.26650/JARHS2024-1407047   IUP :10.26650/JARHS2024-1407047    Tam Metin (PDF)

AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ

Ayşenur Bilgehan, Gül Özhan

Amaç: Çalışmalar, tarım ilaçlarına maruziyetin otizm, beyin yaşlanması ve nörodejeneratif hastalıklar gibi sağlık sorunları ile ilişkili olabileceğini bildirmektedir. Yaygın kullanılan tarım ilaçlarından biri de strobilurin türevi bir fungusit olan azoksistrobin (AZS)’dir. Araştırmalar, çeşitli dokularda AZS’nin toksik etkilerini gösterse de nörotoksik etkilerin altında yatan temel mekanizmalar halen tam olarak anlaşılamamıştır. Tarım ilaçlarına maruziyetin sebep olduğu sağlık sorunlarının oksidatif stres ve apoptoz ile ilişkili olabieceği bildirilmektedir. Bu nedenle, nörotoksisite değerlendirmelerinde yaygın kullanılan bir in vitro model olan insan nöroblastoma (SH-SY5Y) hücrelerinde AZS’nin oksidatif hasar oluşturma ve hücre ölümünü indükleme etkisinin değerlendirilmesi amaçlanmıştır.

Gereç ve Yöntem: SH-SY5Y hücreleri 24 saat boyunca AZS’nin farklı konsantrasyonlarına maruz bırakılmıştır. MTT testi ile sitotoksisite değerlendirilirken spesifik floresan boya ile reaktif oksijen türlerinin (ROS) oluşumu ve FITC Annexin V testi ile de hücresel apoptoz seviyeleri incelenmiştir.

Bulgular: AZS’nin IC50 değeri 44.87 μM olarak bulunmuştur. ROS (en az 1,5 kat; p≤0,05) ve apoptoz (en az 5 kat; p≤0,05) seviyeleri 6.25-25 μM AZS maruziyet aralığında doza bağlı artmıştır.

Sonuç: Bulgularımız, AZS’nin neden olduğu nörotoksik etkilerin ROS üretimi ve ROS ile indüklenen apoptozun bir sonucu olabileceğini düşündürmektedir. Ancak, AZS’nin toksik etki potansiyelinin daha iyi anlaşılması, mesleki ve çevresel risk faktörlerinin değerlendirilmesi, AZS kaynaklı nörotoksisiteye neden olan temel mekanizmaların aydınlatılması için ileri araştırmalara ihtiyaç duyulduğu da göz ardı edilmemelidir.

Anahtar Kelimeler: Azoksistobin, apoptoz, oksidatif stres, nörotoksisite
DOI :10.26650/JARHS2024-1407047   IUP :10.26650/JARHS2024-1407047    Tam Metin (PDF)

IN VITRO TOXIC EFFECTS OF AZOXYSTROBIN ON HUMAN NEUROBLASTOMA CELL LINE

Ayşenur Bilgehan, Gül Özhan

Objective: Exposure to agricultural chemicals is associated with health issues such as autism, brain aging, and neurodegenerative diseases. Azoxystrobin (AZS), a strobilurin-derived fungicide, is a commonly used chemical. Although studies have demonstrated that AZS induces toxic effects on various tissues, there is a lack of clear understanding of the mechanisms underlying its neurotoxic effects. The mechanisms linking pesticide exposure to these health outcomes might be oxidative stress and apoptosis. Therefore, we conducted this study to investigate oxidative stress and cell death induction in human neuroblastoma SH-SY5Y cells, a widely established in vitro model for neurotoxicity experiments, after exposure to AZS.

Materials and Methods: SH-SY5Y cells were exposed to different concentrations of AZS for 24 h. Cytotoxicity was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assay, the induction of reactive oxygen species (ROS) was determined using a fluorescent dye, and cell apoptosis was detected using the FITC Annexin V assay.

Results: The half-maximal inhibitory concentration value of AZS was 44.87 μM. The levels of ROS (at least 1.5-fold; p≤0.05) and apoptosis (at least 5-fold; p≤0.05) increased in a dose-dependent manner at AZS exposure concentrations of 6.25-25 μM.

Conclusion: AZS-induced neurotoxic effects may be a consequence of ROS generation and ROS-induced apoptosis. However, the mechanisms underlying AZS-induced neurotoxicity should be evaluated by further studies to gain a deeper understanding of its toxic capacity and conduct evaluations of occupational and environmental risks.

Anahtar Kelimeler: Azoxystrobin, apoptosis, oxidative stress, neurotoxicity

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DIŞA AKTAR



APA

Bilgehan, A., & Özhan, G. (2024). AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 7(3), 214-219. https://doi.org/10.26650/JARHS2024-1407047


AMA

Bilgehan A, Özhan G. AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi. 2024;7(3):214-219. https://doi.org/10.26650/JARHS2024-1407047


ABNT

Bilgehan, A.; Özhan, G. AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, [Publisher Location], v. 7, n. 3, p. 214-219, 2024.


Chicago: Author-Date Style

Bilgehan, Ayşenur, and Gül Özhan. 2024. “AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7, no. 3: 214-219. https://doi.org/10.26650/JARHS2024-1407047


Chicago: Humanities Style

Bilgehan, Ayşenur, and Gül Özhan. “AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7, no. 3 (Dec. 2024): 214-219. https://doi.org/10.26650/JARHS2024-1407047


Harvard: Australian Style

Bilgehan, A & Özhan, G 2024, 'AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ', Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 7, no. 3, pp. 214-219, viewed 22 Dec. 2024, https://doi.org/10.26650/JARHS2024-1407047


Harvard: Author-Date Style

Bilgehan, A. and Özhan, G. (2024) ‘AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ’, Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 7(3), pp. 214-219. https://doi.org/10.26650/JARHS2024-1407047 (22 Dec. 2024).


MLA

Bilgehan, Ayşenur, and Gül Özhan. “AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 7, no. 3, 2024, pp. 214-219. [Database Container], https://doi.org/10.26650/JARHS2024-1407047


Vancouver

Bilgehan A, Özhan G. AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];7(3):214-219. Available from: https://doi.org/10.26650/JARHS2024-1407047 doi: 10.26650/JARHS2024-1407047


ISNAD

Bilgehan, Ayşenur - Özhan, Gül. “AZOXYSTROBİN’İN İNSAN NÖROBLASTOMA HÜCRE HATTI ÜZERİNDEKİ İN VİTRO TOKSİK ETKİLERİ”. Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7/3 (Dec. 2024): 214-219. https://doi.org/10.26650/JARHS2024-1407047



ZAMAN ÇİZELGESİ


Gönderim19.12.2023
Kabul15.03.2024
Çevrimiçi Yayınlanma22.10.2024

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