Research Article


DOI :10.5152/EurJBiol.2017.1703   IUP :10.5152/EurJBiol.2017.1703    Full Text (PDF)

An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats

Tunç ÇatalSevim TunalıŞehnaz BolkentRefiye Yanardağ

The protective effects of a combination of antioxidants on histopathological changes and biochemical parameters were examined in D-galactosamine (D-GaIN)-induced hepatotoxicity in rats. Physiological saline solution was injected intraperitoneally into the control group, while D-GaIN (500 mg kg-1) was administered intraperitoneally into the experimental animals. The combination of 100 mg/kg/day ascorbic acid, 100 mg/kg/day alpha tocopherol, 15 mg/kg/day beta carotene, and 0.2 mg/kg/day sodium selenate was administered orally to intact control rats for 3 days. The same antioxidant combination was given to the D-GaIN group. Liver and blood samples were used for histopathological and biochemical assays. Liver tissues were significantly damaged by D-GaIN administration based on the histopathological findings. Serum aspartate and alanine transaminase, gamma glutamyl transpeptidase, alkaline phosphatase, lactate dehydrogenase, sialic acid, and uric acid levels increased. Serum and liver glutathione levels decreased, and serum superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase, catalase activities as well as lipid peroxidation values increased in the D-GaIN group. In contrast, administration of the antioxidants reversed the histopathological and biochemical changes in the liver of animals administered D-GaIN. In conclusion, the administration of a combination of antioxidants suppressed histopathological changes and biochemical parameters in rats given D-GaIN.


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APA

Çatal, T., Tunalı, S., Bolkent, Ş., & Yanardağ, R. (2017). An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats. European Journal of Biology, 76(1), 14-19. https://doi.org/10.5152/EurJBiol.2017.1703


AMA

Çatal T, Tunalı S, Bolkent Ş, Yanardağ R. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats. European Journal of Biology. 2017;76(1):14-19. https://doi.org/10.5152/EurJBiol.2017.1703


ABNT

Çatal, T.; Tunalı, S.; Bolkent, Ş.; Yanardağ, R. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats. European Journal of Biology, [Publisher Location], v. 76, n. 1, p. 14-19, 2017.


Chicago: Author-Date Style

Çatal, Tunç, and Sevim Tunalı and Şehnaz Bolkent and Refiye Yanardağ. 2017. “An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats.” European Journal of Biology 76, no. 1: 14-19. https://doi.org/10.5152/EurJBiol.2017.1703


Chicago: Humanities Style

Çatal, Tunç, and Sevim Tunalı and Şehnaz Bolkent and Refiye Yanardağ. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats.” European Journal of Biology 76, no. 1 (May. 2025): 14-19. https://doi.org/10.5152/EurJBiol.2017.1703


Harvard: Australian Style

Çatal, T & Tunalı, S & Bolkent, Ş & Yanardağ, R 2017, 'An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats', European Journal of Biology, vol. 76, no. 1, pp. 14-19, viewed 23 May. 2025, https://doi.org/10.5152/EurJBiol.2017.1703


Harvard: Author-Date Style

Çatal, T. and Tunalı, S. and Bolkent, Ş. and Yanardağ, R. (2017) ‘An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats’, European Journal of Biology, 76(1), pp. 14-19. https://doi.org/10.5152/EurJBiol.2017.1703 (23 May. 2025).


MLA

Çatal, Tunç, and Sevim Tunalı and Şehnaz Bolkent and Refiye Yanardağ. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats.” European Journal of Biology, vol. 76, no. 1, 2017, pp. 14-19. [Database Container], https://doi.org/10.5152/EurJBiol.2017.1703


Vancouver

Çatal T, Tunalı S, Bolkent Ş, Yanardağ R. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats. European Journal of Biology [Internet]. 23 May. 2025 [cited 23 May. 2025];76(1):14-19. Available from: https://doi.org/10.5152/EurJBiol.2017.1703 doi: 10.5152/EurJBiol.2017.1703


ISNAD

Çatal, Tunç - Tunalı, Sevim - Bolkent, Şehnaz - Yanardağ, Refiye. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats”. European Journal of Biology 76/1 (May. 2025): 14-19. https://doi.org/10.5152/EurJBiol.2017.1703



TIMELINE


Submitted24.08.2017
Accepted19.09.2017

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