Research Article


DOI :10.26650/EurJBiol.2019.0035   IUP :10.26650/EurJBiol.2019.0035    Full Text (PDF)

Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis

Elif KaracaoğluGözde GirginGüldeniz SelmanoğluTerken Baydar

Objective: Acute pancreatitis (AP) is an inflammatory disease of the pancreas resulting from auto-activation of digestive enzymes and damage to the pancreatic parenchyma. Reactive oxygen species (ROS) play an important role in the progression of AP. In the present study, we aimed to evaluate epigallocatechin-3 gallate (EGCG) in reducing the inflammatory reaction and tissue damage in experimental AP rat model. Materials and Methods: Amylase, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels were measured. Histopathological, immunohistochemical analyses of apoptotic cells, CD-8α and CD-68 were performed. Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) were determined in hemolysates. Results: Cerulein+EGCG treatment did not cause decreases in the amylase levels. IL-6 levels decreased in cerulein+EGCG group, however, TNF-α levels increased. No changes were observed in SOD activity by EGCG treatment, CAT and GST activities increased. EGCG treatment caused severe edema, inflammation and fat necrosis after cerulein-induced pancreatitis. Apoptosis in pancreas, CD8-α and CD-68 positive cells increased in EGCG treatment after pancreatitis induction. Conclusion: It may be suggested that EGCG showed a pro-oxidant effect, in contrast to the expected in the pancreatitis model when compared to a positive control. It can be concluded that overconsumption of EGCG should be avoided in pancreatitis conditions.

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APA

Karacaoğlu, E., Girgin, G., Selmanoğlu, G., & Baydar, T. (2019). Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis. European Journal of Biology, 78(2), 125-132. https://doi.org/10.26650/EurJBiol.2019.0035


AMA

Karacaoğlu E, Girgin G, Selmanoğlu G, Baydar T. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis. European Journal of Biology. 2019;78(2):125-132. https://doi.org/10.26650/EurJBiol.2019.0035


ABNT

Karacaoğlu, E.; Girgin, G.; Selmanoğlu, G.; Baydar, T. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis. European Journal of Biology, [Publisher Location], v. 78, n. 2, p. 125-132, 2019.


Chicago: Author-Date Style

Karacaoğlu, Elif, and Gözde Girgin and Güldeniz Selmanoğlu and Terken Baydar. 2019. “Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis.” European Journal of Biology 78, no. 2: 125-132. https://doi.org/10.26650/EurJBiol.2019.0035


Chicago: Humanities Style

Karacaoğlu, Elif, and Gözde Girgin and Güldeniz Selmanoğlu and Terken Baydar. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis.” European Journal of Biology 78, no. 2 (Nov. 2024): 125-132. https://doi.org/10.26650/EurJBiol.2019.0035


Harvard: Australian Style

Karacaoğlu, E & Girgin, G & Selmanoğlu, G & Baydar, T 2019, 'Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis', European Journal of Biology, vol. 78, no. 2, pp. 125-132, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2019.0035


Harvard: Author-Date Style

Karacaoğlu, E. and Girgin, G. and Selmanoğlu, G. and Baydar, T. (2019) ‘Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis’, European Journal of Biology, 78(2), pp. 125-132. https://doi.org/10.26650/EurJBiol.2019.0035 (22 Nov. 2024).


MLA

Karacaoğlu, Elif, and Gözde Girgin and Güldeniz Selmanoğlu and Terken Baydar. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis.” European Journal of Biology, vol. 78, no. 2, 2019, pp. 125-132. [Database Container], https://doi.org/10.26650/EurJBiol.2019.0035


Vancouver

Karacaoğlu E, Girgin G, Selmanoğlu G, Baydar T. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis. European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];78(2):125-132. Available from: https://doi.org/10.26650/EurJBiol.2019.0035 doi: 10.26650/EurJBiol.2019.0035


ISNAD

Karacaoğlu, Elif - Girgin, Gözde - Selmanoğlu, Güldeniz - Baydar, Terken. Antioxidant Effects of Epigallocatechin Gallate in Cerulein-Induced Pancreatitis”. European Journal of Biology 78/2 (Nov. 2024): 125-132. https://doi.org/10.26650/EurJBiol.2019.0035



TIMELINE


Submitted01.10.2019
Accepted06.11.2019
Published Online06.12.2019

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