Benzyl Alcohol Increases Diffusion Limit of Nuclear  Membrane in Saccharomyces cerevisiae Cells

Ergüden Bengü

doi:https://dx.doi.org/10.26650/EurJBiol.2022.1058174

Research Article

DOI :10.26650/EurJBiol.2022.1058174 IUP :10.26650/EurJBiol.2022.1058174 Full Text (PDF)

Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells

Objective: Fungi are invasive species responsible for infections in many people around the world and which severely affect the immune system. The opportunistic pathogenic species, such as Candida species and Aspergillus fumigatus, can cause death in people with weakened immune systems. Natural medicines derived from plants are often used to treat fungal diseases. In connection with our efforts to unearth possible cellular targets of antimicrobial agents, in this study, we aimed to determine the functional consequences of benzyl alcohol treatment on the nuclear membrane. Materials and Methods: We analysed the nuclear membrane distortions caused by benzyl alcohol in Saccharomyces cerevisiae cells using Nup49-GFP reporter strain. We also studied cellular distributions of various fluorescently tagged nuclearcytoplasmic shuttling proteins to determine any functional disturbances in nuclear pore complexes upon benzyl alcohol treatment. Localization of 51.5 kDa protein LexA-NES-GFP and 61.8 kDa protein Pho4(Δ157-164)-GFP to the nucleus in yeast cells was key for evaluating the effect upon diffusion limit of pores. Results: By analyzing the distribution of fluorescently tagged nuclear localization signal or nuclear export signals bearing reporter proteins between the nucleus and cytoplasm, we have shown that the nuclear membrane becomes leaky upon benzyl alcohol treatment. Conclusion: The diffusion limit across the nuclear membrane in yeast cells is increased upon benzyl alcohol treatment. We believe that these findings not only increase our understanding of the mode of action of benzyl alcohol bearing antifungal agents, but also help widening their use.

Keywords: Benzyl alcohol, yeast, antifungal activity, nuclear membrane, nuclear pore complex

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APA

Ergüden, B. (2022). Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells. European Journal of Biology, 81(1), 26-30. https://doi.org/10.26650/EurJBiol.2022.1058174

AMA

Ergüden B. Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells. European Journal of Biology. 2022;81(1):26-30. https://doi.org/10.26650/EurJBiol.2022.1058174

ABNT

Ergüden, B. Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells. European Journal of Biology, [Publisher Location], v. 81, n. 1, p. 26-30, 2022.

Chicago: Author-Date Style

Ergüden, Bengü,. 2022. “Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells.” European Journal of Biology 81, no. 1: 26-30. https://doi.org/10.26650/EurJBiol.2022.1058174

Chicago: Humanities Style

Ergüden, Bengü,. “Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells.” European Journal of Biology 81, no. 1 (May. 2024): 26-30. https://doi.org/10.26650/EurJBiol.2022.1058174

Harvard: Australian Style

Ergüden, B 2022, 'Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells', European Journal of Biology, vol. 81, no. 1, pp. 26-30, viewed 4 May. 2024, https://doi.org/10.26650/EurJBiol.2022.1058174

Harvard: Author-Date Style

Ergüden, B. (2022) ‘Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells’, European Journal of Biology, 81(1), pp. 26-30. https://doi.org/10.26650/EurJBiol.2022.1058174 (4 May. 2024).

MLA

Ergüden, Bengü,. “Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells.” European Journal of Biology, vol. 81, no. 1, 2022, pp. 26-30. [Database Container], https://doi.org/10.26650/EurJBiol.2022.1058174

Vancouver

Ergüden B. Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells. European Journal of Biology [Internet]. 4 May. 2024 [cited 4 May. 2024];81(1):26-30. Available from: https://doi.org/10.26650/EurJBiol.2022.1058174 doi: 10.26650/EurJBiol.2022.1058174

ISNAD

Ergüden, Bengü. “Benzyl Alcohol Increases Diffusion Limit of Nuclear Membrane in Saccharomyces cerevisiae Cells”. European Journal of Biology 81/1 (May. 2024): 26-30. https://doi.org/10.26650/EurJBiol.2022.1058174

Volume 81, Issue 12022, P. 26-30

TIMELINE

Submitted	15.01.2022
Accepted	05.04.2022
Published Online	29.04.2022

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