Research Article

DOI :10.26650/EurJBiol.2024.1480514 IUP :10.26650/EurJBiol.2024.1480514 Full Text (PDF)

Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds

Belmina Šarić Medić, Anesa Jerković Mujkić, Berina Cubara, Adaleta Durmić Pašić, Jasna Hanjalić Kurtović, Kasim Bajrovic, Elma Omeragić, Mirza Dedić, Faruk Bogunić, Lejla Pojskić

Objective: Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts (aqueous, 50% and 80% ethanol) of three Iris species (I. pumila, while I. reichenbachii and I. illyrica are endemic) from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA strain).

Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography (HPLC), and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations.

Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger (isorhamnetin) or the same (hesperidin) as the positive control ceftobiprole.

Conclusion: This in vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.

Keywords: Plant bioactive compounds, Methicillin-resistant Staphylococcus aureus, Molecular docking, Minimum inhibitory concentration

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APA

Medić, B.Š., Jerković Mujkić, A., Cubara, B., Durmić Pašić, A., Kurtović, J.H., Bajrovic, K., Omeragić, E., Dedić, M., Bogunić, F., & Pojskić, L. (2024). Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2024.1480514

AMA

Medić B Š, Jerković Mujkić A, Cubara B, Durmić Pašić A, Kurtović J H, Bajrovic K, Omeragić E, Dedić M, Bogunić F, Pojskić L. Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds. European Journal of Biology. 2024;0(0):-. https://doi.org/10.26650/EurJBiol.2024.1480514

ABNT

Medić, B.Š.; Jerković Mujkić, A.; Cubara, B.; Durmić Pašić, A.; Kurtović, J.H.; Bajrovic, K.; Omeragić, E.; Dedić, M.; Bogunić, F.; Pojskić, L. Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2024.

Chicago: Author-Date Style

Medić, Belmina Šarić, and Anesa Jerković Mujkić and Berina Cubara and Adaleta Durmić Pašić and Jasna Hanjalić Kurtović and Kasim Bajrovic and Elma Omeragić and Mirza Dedić and Faruk Bogunić and Lejla Pojskić. 2024. “Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2024.1480514

Chicago: Humanities Style

Medić, Belmina Šarić, and Anesa Jerković Mujkić and Berina Cubara and Adaleta Durmić Pašić and Jasna Hanjalić Kurtović and Kasim Bajrovic and Elma Omeragić and Mirza Dedić and Faruk Bogunić and Lejla Pojskić. “Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds.” European Journal of Biology 0, no. 0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1480514

Harvard: Australian Style

Medić, BŠ & Jerković Mujkić, A & Cubara, B & Durmić Pašić, A & Kurtović, JH & Bajrovic, K & Omeragić, E & Dedić, M & Bogunić, F & Pojskić, L 2024, 'Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 25 Nov. 2024, https://doi.org/10.26650/EurJBiol.2024.1480514

Harvard: Author-Date Style

Medić, B.Š. and Jerković Mujkić, A. and Cubara, B. and Durmić Pašić, A. and Kurtović, J.H. and Bajrovic, K. and Omeragić, E. and Dedić, M. and Bogunić, F. and Pojskić, L. (2024) ‘Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2024.1480514 (25 Nov. 2024).

MLA

Medić, Belmina Šarić, and Anesa Jerković Mujkić and Berina Cubara and Adaleta Durmić Pašić and Jasna Hanjalić Kurtović and Kasim Bajrovic and Elma Omeragić and Mirza Dedić and Faruk Bogunić and Lejla Pojskić. “Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds.” European Journal of Biology, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1480514

Vancouver

Medić BŠ, Jerković Mujkić A, Cubara B, Durmić Pašić A, Kurtović JH, Bajrovic K, Omeragić E, Dedić M, Bogunić F, Pojskić L. Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds. European Journal of Biology [Internet]. 25 Nov. 2024 [cited 25 Nov. 2024];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2024.1480514 doi: 10.26650/EurJBiol.2024.1480514

ISNAD

Medić, BelminaŠarić - Jerković Mujkić, Anesa - Cubara, Berina - Durmić Pašić, Adaleta - Kurtović, JasnaHanjalić - Bajrovic, Kasim - Omeragić, Elma - Dedić, Mirza - Bogunić, Faruk - Pojskić, Lejla. “Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds”. European Journal of Biology 0/0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1480514

TIMELINE

Submitted	15.05.2024
Accepted	19.08.2024
Published Online	19.09.2024

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