Research Article


DOI :10.26650/EurJBiol.2022.1109068   IUP :10.26650/EurJBiol.2022.1109068    Full Text (PDF)

Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety

Sara MessadSouhila BensmailOmar SalhiDjamila Djouahra Fahem

Objective: The production of olive oil in Algeria is of great importance. The choice of the extraction method is crucial in order to achieve the best yields of high quality oil. This work aims to study the effect of the extraction method on the sensory and physicochemical characteristics and the antioxidant and antimicrobial activities of three types of olive oil derived from the same sample of olive fruits (Chemlal variety). Materials and Methods: Several physicochemical and sensory parameters of olive oil samples were evaluated by referenced methods (ISO, IOC, AFNOR, etc.). The fatty acid composition of oils was determined by gas chromatography. Total phenolic content and antioxidant activity were estimated using the Folin-Ciocalteu assay and FRAP test, respectively. Antimicrobial activity analysis was performed using the agar diffusion technique against five strains. Results: The analyses revealed that olive oil obtained by manual extraction is classified in the extra virgin category, according to the International Olive Oil Council. The two other oils, traditional and industrial, were of virgin class. Fatty acid composition shows significant differences between the three oils which are characterized by a remarkable richness of phenolic compounds (447-528 mg GAE/kg oil). The best antioxidant activity was noted in the manual olive oil (PR0.5=78.60 µg/mL). Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ATCC 6538 were more sensitive to action of the manual oil compared to other bacterial and fungal strains tested, with inhibition zones of 15.84±0.07 and 15.31±0.20 mm, respectively. This activity was significantly decreased by evaluating antimicrobial effect of the other oils. Conclusion: The method of extraction significantly affects the properties of olive oil, where the manual method preserves the best quality of oil.


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References

  • 1. Serreli G, Deiana M. Biological relevance of extra virgin olive oil polyphenols metabolites. Antioxidants 2018; 7(12): 170. google scholar
  • 2. Otero P, Garcia-Oliveira P, Carpena M, Barral-Martinez M, Chamorro F, Echave J, et al. Applications of by-products from the olive oil pro-cessing: Revalorization strategies based on target molecules and green extraction technologies. Trends Food Sci Technol 2021; 116: 1084-104. google scholar
  • 3. Petrakis C. Olive oil extraction. Boskou D, editor. Olive Oil: Chemis-try and Technology. 2nd ed. AOCS Press; 2006.p.191-23. google scholar
  • 4. Souilem S, El-Abbassi A, Kiai H, Hafidi A, Sayadi S, Galanakis, CM. Olive oil production sector: Environmental effects and sustainabil-ity challenges. Galanakis CM, editor. Olive mill waste. Academic Press; 2017.p.1-28. google scholar
  • 5. Saffar Taluri S, Jafari SM, Bahrami A. Evaluation of changes in the quality of extracted oil from olive fruits stored under different tem-peratures and time intervals. Sci Rep 2019; 9(1): 19688. google scholar
  • 6. Huang CL, Sumpio BE. Olive Oil, the Mediterranean Diet, and Car-diovascular Health. J. Am. Coll. Surg 2008; 207(3): 407-16. google scholar
  • 7. Titouh K, Mazari A, Ait Meziane MZ. Contribution to improvement of the traditional extraction of olive oil by pressure from whole and stoned olives by addition of a co-adjuvant (talc). OCL 2020; 27, 23. google scholar
  • 8. Acar A, Arslan D. Some technological pretreatments applied during olive oil extraction: Impacts on quality parameters and mi-nor constituents. Glob J Agric Innov Res Dev 2017; 4: 47-7. google scholar
  • 9. International Olive Oil Council (IOC). Sensory analysis of olive oil: method of organoleptic evaluation of virgin olive oil. COI/T.20/ Doc. /Rev.10: 2018. Report No 15. google scholar
  • 10. International Olive Oil Council (IOC). Trade standard applicable to ol-ive oils and olive-pomace oils. COI/T.15/NC /Rev.14: 2019. Report No3. google scholar
  • 11. International Organization for Standardization (ISO). Animal and vegetable fats and oils - Determination of refractive index. Ref. No.EN ISO 6320:2000/AC: 2006 D/E/F. google scholar
  • 12. Journal officiel de la republique algerienne (JORA). Methodes offi-cielles d’analyses physico-chimiques relatives aux corps gras d’orig-ine animales et vegetales (Official Journal of the Algerian Republic (JORA). Official methods of physico-chemical analysis relating to fatty substances of animal and vegetable origin): 2011. Report No64. google scholar
  • 13. International Olive Oil Council (IOC). Spectrophotometer investi-gation in the ultraviolet, COI/T.20/ Doc./Rev.3: 2015. Report No 19. google scholar
  • 14. Reglement relatif aux caracteristiques des huiles d’olive et des huiles de grignons d’olive ainsi qu’aux methodes d’analyse y af-ferentes (JOL 248 du 5.9.1991, p : 114): 1991. Report No 2568/91. google scholar
  • 15. French Association for Standardization (AFNOR). Fats - oilseeds -derived products, 4th ed, Paris: 1984. p: 459. google scholar
  • 16. Minguez-Mosquera IM, Rejano-Navarro L, Gandul-Rojas B, San-chez Gomez AH, Garrido-Fernandez J. Color-pigment correlation in virgin olive oil. J Am Oil Chem Soc 1991; 68(5): 33-6. google scholar
  • 17. International Olive Oil Council (IOC). Preparation des esters me-thyliques d’acides gras de l’huile d’olive et de l’huile de grignons d’ol-ive. Conseil Oleicole International. COI/T.20/Doc.: 2001. Report No 24. google scholar
  • 18. Pirisi FM, Cabras P, Cao CF, Migliorini M, Muggelli M. Phenolic com-pounds in virgin olive oil. 2. Reappraisal of the extraction, HPLC separation, and quantification procedures. J Agric Food Chem 2000; 48(4): 1191-916. google scholar
  • 19. Singleton VL, Rossi JA. Colorimetry of total phenolics with phos-phomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic 1965; 16(3): 144-58. google scholar
  • 20. Topçu G, Ay M, Bilici A, Sarıkürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chem 2007; 103(3): 816-22. google scholar
  • 21. Medina E, de Castro A, Romero C, Brenes M. Comparison of the concentrations of phenolic compounds in olive oils and other plant oils: Correlation with antimicrobial activity. J Agric Food Chem 2006; 54(14): 4954-961. google scholar
  • 22. Bouzid W, Yahia M., Abdeddaim M., Aberkane MC, Ayachi A. Evalu-ation de l’activite antioxydante et antimicrobienne des extraits de l’aubepine monogyne. Leban Sci J 2011; 12(1): 59-69. google scholar
  • 23. Ponce AG, Fritz R, del Valle C, Roura SI. Antimicrobial activity of es-sential oils on the native microflora of organic Swiss chard. LWT. Food Sci. Technol. J 2003; 36(7): 679-84. google scholar
  • 24. Ouelhadj A, Salem LA, Djenane D. Activite antibacterienne de l’huile essentielle de Pelargonium x asperum et son potentiel syn-ergique avec la nisine. Phytotherapie 2019; 17(3): 140-8. google scholar
  • 25. Bianchi G. Extraction systems and olive oil. OCL 1999; 6(1): 49-55. google scholar
  • 26. Pouyet B, Ollivier V. Reglementations sur l’etiquetage et la presen-tation des huiles d’olive. OCL 2014; 21(5): D508. google scholar
  • 27. Amirante P, Clodoveo ML, Dugo G, Leone A, Tamborrino A. Ad-vance technology in virgin olive oil production from traditional and de-stoned pastes: Influence of the introduction of a heat ex-changer on oil quality. Food Chem 2006; 98(4): 797-05. google scholar
  • 28. Codex Alimentarius. Norme pour les huiles d’olive et les huiles de grignons d’olive. CODEX STAN 33-1981 Adoptee en 1981. Revisee en 1989, 2003, 2015, 2017. Amendee en 2009, 2013. Codex STAN 33-1981. Rev 2017. google scholar
  • 29. Santos OV, Correa NCF, Soares FASM, Gioielli LA, Costa CEF, Lannes SCS. Chemical evaluation and thermal behavior of Brazil nut oil obtained by different extraction processes. Food Res Int 2012; 47(2): 253-8. google scholar
  • 30. Sekour B. Phyto-protection de l’huile d’olive vierge par ajout des plantes vegetales (thym, ail, romarin) (Phyto-protection of virgin ol-ive oil by adding vegetable plants (thyme, garlic, rosemary). Universi-ty of Boumerdes, Magister thesis. 2012. google scholar
  • 31. Benincasa C, De Nino A, Lombardo N, Perri E, Sindona G, Tagarel-li A. Assay of aroma active components of virgin olive oils from Southern Italian Regions by SPME-GC/Ion Trap Mass Spectrome-try. J Agric Food Chem 2003; 51(3):733-41. google scholar
  • 32. Essiari M, Zouhair R, Chimi H. Contribution a l’etude de la typicite des huiles d’olives vierges produites dans la region de Sais (Maroc). Olivae 2014; 119: 8-22. google scholar
  • 33. Abdullah F, Mohammed E. Modelisation de la repartition du trans-fert des metaux lourds et des oligoelements dans les sols forestiers, l’huile d’argan et dans les differentes parties d’arganier (Modeling of the distribution of the transfer of heavy metals and trace elements in forest soils, argan oil and in the different parts of the argan tree). Mohammed V-Agdal University, Doctoral thesis. 2012. google scholar
  • 34. Vidal AM, Alcala S, de Torres A, Moya M, Espı'nola F. Industrial pro-duction of a balanced Virgin olive oil. LWT. 2018;97:588-96. google scholar
  • 35. Meftah H, Latrache H, Hamadi F, Hanine H, Zahir H,. El louali M. Comparaison des caracteristiques physicochimiques des huiles d’olives issus de differentes zones de la region Tadla Azilal (Maroc) (Comparison of the physico-chemical characteristics of the olive oil coming from different zones in Tadla Azilal area (Morocco). J Mater Environ Sci 2014; 5(2): 641-6. google scholar
  • 36. Salvador MD, Aranda F, Gömez-Alonso S, Fregapane G. Cornicabra virgin olive oil: a study of five crop seasons. Composition, quality and oxidative stability. Food Chem 2001; 74(3): 267-74. google scholar
  • 37. Psomiadou E, Tsimidou M. Pigments in Greek virgin olive oils: oc-currence and levels. J Sci Food Agric 2001; 81(7): 640-7. google scholar
  • 38. Giuffrida D, Salvo F, Salvo A, Pera LL, Dugo G. Pigments compo-sition in monovarietal virgin olive oils from various sicilian olive varieties. Food Chem 2007; 101(2): 833-7. google scholar
  • 39. Tanouti K, Elamrani A, Serghini-Caid H, khalid A, Bahetta Y, Benali A, et al. Caracterisation d’huiles d’olive produites dans des coopera-tives pilotes (Lakrarma et Kenine) au niveau du Maroc Oriental. Les Technologies De Laboratoire 2010; 5(18): 18-26. google scholar
  • 40. Baccouri B, Guerfel M, Zarrouk W, Taamalli W, Daoud D, Zarrouk M. Wild olive (Olea europaea L.) selection for quality oil production. J. Food Biochem 2011; 35(1): 161-76. google scholar
  • 41. Sait S. Activites antioxydante et antibacterienne des huiles d’oleas-tres (Olea europaea var. oleaster) de la region de Bejaıa (Antioxidant and antibacterial activities of oleaster oils (Olea europaea var. oleast-er) from the Bejaıa region). University of Bejaia, Magister thesis. 2012. google scholar
  • 42. Baccouri B, Temime SB, Taamalli W, Daoud D, M’Sallem M, Zarrouk M. Analytical characteristics of virgin olive oils from two new vari-eties obtained by controlled crossing on Meski variety. J Food Lip-ids 2007; 14(1): 19-34. google scholar
  • 43. Hashempour A, Ghazvini RF, Bakhshi D, Sanam SA. Fatty acids com-position and pigments changing of virgin olive oil (Olea europea L.) in five cultivars grown in Iran. Aust J Crop Sci 2010; 4(4): 258-63. google scholar
  • 44. Allalout A, Krichene D, Methenni K, Taamalli A, Oueslati I, Daoud D, et al. Characterization of virgin olive oil from Super Intensive Spanish and Greek varieties grown in northern Tunisia. Scientia Horticulturae 2009;120(1): 77-83. google scholar
  • 45. Tanouti K, Serghini-Caid H, Chaieb E, Benali A, Harkous M, Elamrani A. Amelioration qualitative d’huiles d’olive produites dans le Ma-roc oriental. Les Technologies de Laboratoire. 2011; 6(22): 1-12. google scholar
  • 46. Viljanen K, Sundberg S, Ohshima T, Heinonen M. Carotenoids as antioxidants to prevent photooxidation. Eur J Lipid Sci Technol 2002; 104(6): 353-9. google scholar
  • 47. Hadj Sadok T, Rebiha K, Teriki D. Caracterisation physico-chimique et organoleptique des huiles d’olive vierges de quelques varietes algeriennes. Agrobiologia 2018; 8(1): 706-18. google scholar
  • 48. Faci M, Hedjal M, Douzane M, Sevim D, Köseoğlu O, Tamendjari A. Locations effects on the quality of Chemlal and Azeradj olives grown in Algeria. J Am Oil Chem Soc 2021; 98(5): 551-66. google scholar
  • 49. Zaringhalami S, Ebrahimi M, Piravi Vanak Z, Ganjloo A. Effects of cultivar and ripening stage of Iranian olive fruit on bioactive com-pounds and antioxidant activity of its virgin oil. Int. Food Res. J 2015; 22(5): 1961-7. google scholar
  • 50. Gutierrez F, Jı'menez B, Ruı'z A, Albi MA. Effect of olive ripeness on the oxidative stability of virgin olive oil extracted from the varieties Picual and Hojiblanca and on the different components involved. J Agric Food Chem 1999; 47(1): 121-7. google scholar
  • 51. Baccouri O, Guerfel M, Baccouri B, Cerretani L, Bendini A, Lercker G, et al. Chemical composition and oxidative stability of Tunisian monovarietal virgin olive oils with regard to fruit ripening. Food Chem 2008; 109(4): 743-54. google scholar
  • 52. Matos L, Pereira J, Andrade P, Seabra R, Oliveira M. Evaluation of a numerical method to predict the polyphenols content in monova-rietal olive oils. Food Chem 2007; 102(3): 976-83. google scholar
  • 53. Douzane M, Daas MS, Meribai A, Guezil AH, Abdi A, Tamendjari A. Physico-chemical and sensory evaluation of virgin olive oils from several Algerian olive-growing regions. OCL 2021; 28: 55. google scholar
  • 54. Douzane M, Tamendjari A, Abdi AK, Daas MS, Mehdid F, Bellal MM. Phenolic compounds in mono-cultivar extra virgin olive oils from Algeria. Grasas y Aceites 2013; 64(3): 285-94. google scholar
  • 55. Laincer F, Laribi R, Tamendjari A, Arrar L, Rovellini P, Venturini S. Olive oils from Algeria: Phenolic compounds, antioxidant and anti-bacterial activities. Grasas y Aceites 2014; 65(1): e001. google scholar
  • 56. Medjkouh L, Tamendjari A, Keciri S, Santos J, Nunes MA, Oliveira MBPP. The effect of the olive fruit fly (Bactrocera oleae) on quality parameters, and antioxidant and antibacterial activities of olive oil. Food Funct 2016; 7(6): 2780-8. google scholar
  • 57. Merouane A, Noui A, Medjahed H, Nedjari Benhadj Ali K, Saadi A. Activite antioxydante des composes phenoliques d’huile d’olive extraite par methode traditionnelle. Int J Bio Chem Sci 2015; 8(4): 1865-70. google scholar
  • 58. Ranalli A, Ferrante ML, De Mattia G, Costantini N. Analytical evalu-ation of virgin olive oil of first and second extraction. J Agric Food Chem 1999; 47(2): 417-24. google scholar
  • 59. Morello JR, Motilva MJ, Tovar MJ, Romero MP. Changes in commer-cial virgin olive oil (cv Arbequina) during storage, with special em-phasis on the phenolic fraction. Food Chem 2004; 85(3): 357-64. google scholar
  • 60. Aparicio R, Roda L, Albi MA, Gutierrez F. Effect of various com-pounds on virgin olive oil stability measured by Rancimat. J Agric Food Chem 1999; 47(10): 4150-5. google scholar
  • 61. Benlemlih M, Ghanam J, Joyeux H. Polyphenols d’huile d’olive, tresors sante!: polyphenols aux actions antioxydantes, anti-inflam-matoires, anticancereuses, anti-vieillissement et protectrices car-dio-vasculaires. 2nd ed, Medicatrix, France; 2016. p.208. google scholar
  • 62. Tunçel G, Nergiz C. Antimicrobial effect of some olive phenols in a laboratory medium. Lett Appl Microbiol 1993; 17(6): 300-12. google scholar
  • 63. Melguizo-Rodnguez L, Illescas-Montes R, Costela-Ruiz VJ, Ramos-Tor-recillas J, de Luna-Bertos E, Garcı'a-Martı'nez O, et al. Antimicrobial properties of olive oil phenolic compounds and their regenerative capacity towards fibroblast cells. J Tissue Viability 2021; 30(3): 372-78. google scholar
  • 64. Djedioui A. Caracterisation d’une huile d’olive vierge algerienne d’une variete cultivee dans la region de Skikda (Characterization of an Algerian virgin olive oil of a variety grown in the Skikda region). University of Annaba, Doctoral thesis. 2018. google scholar
  • 65. Laribi R. Les composes phenoliques de quelques varietes de l’huile algeriennes: identification et proprietes (The phenolic compounds of some varieties of Algerian oil: identification and properties). Uni-versity of Setif, Doctoral thesis. 2015. google scholar
  • 66. Romero C, Medina E, Vargas J, Brenes M, De Castro A. In vitro activ-ity of olive oil polyphenols against Helicobacter pylori. J Agric Food Chem 2007; 55(3): 680-6. google scholar
  • 67. Karaosmanoglu H, Soyer F, Ozen B, Tokatli F. Antimicrobial and an-tioxidant activities of Turkish extra virgin olive oils. J Agric Food Chem 2010; 58(14): 8238-45. google scholar
  • 68. Toty AA, Guessennd N, Bahi C, Kra AK, Tokore DA, Dosso M. Eval-uation in-vitro de l’activite antibacterienne de l’extrait aqueux de l’ecorce de tronc de Harungana madagascariensis sur la croissance de souches multi-resistantes. Bulletin de la societe royale des sci-ences de Liege 2013; 82:12-21. google scholar
  • 69. Carvalho CCCR, Caramujo MJ. Ancient procedures for the High-Tech World: Health benefits and antimicrobial compounds from the Mediterranean Empires. TOBIOTJ 2008; 2(1): 235-46. google scholar
  • 70. Dilika F, Bremner PD, Meyer JJM. Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia 2000; 71(4): 450-2. google scholar
  • 71. Bisignano G, Tomaino A, Cascio RL, Crisafi G, Uccella N, Saija A. On the in-vitro antimicrobial activity of oleuropein and hydroxytyro-sol. J. Pharm. Pharmacol 2010; 51(8): 971-74. google scholar
  • 72. Tuck KL, Hayball PJ. Major phenolic compounds in olive oil: metab-olism and health effects. J. Nutr. Biochem 2002; 13(11): 636-44. google scholar
  • 73. Cicerale S, Conlan XA, Barnett NW, Keast RSJ. The concentration of oleocanthal in olive oil waste. Nat. Prod. Res 2011; 25(5): 542-8. google scholar

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APA

Messad, S., Bensmail, S., Salhi, O., & Djouahra Fahem, D. (2022). Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety. European Journal of Biology, 81(1), 58-67. https://doi.org/10.26650/EurJBiol.2022.1109068


AMA

Messad S, Bensmail S, Salhi O, Djouahra Fahem D. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety. European Journal of Biology. 2022;81(1):58-67. https://doi.org/10.26650/EurJBiol.2022.1109068


ABNT

Messad, S.; Bensmail, S.; Salhi, O.; Djouahra Fahem, D. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety. European Journal of Biology, [Publisher Location], v. 81, n. 1, p. 58-67, 2022.


Chicago: Author-Date Style

Messad, Sara, and Souhila Bensmail and Omar Salhi and Djamila Djouahra Fahem. 2022. “Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety.” European Journal of Biology 81, no. 1: 58-67. https://doi.org/10.26650/EurJBiol.2022.1109068


Chicago: Humanities Style

Messad, Sara, and Souhila Bensmail and Omar Salhi and Djamila Djouahra Fahem. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety.” European Journal of Biology 81, no. 1 (May. 2024): 58-67. https://doi.org/10.26650/EurJBiol.2022.1109068


Harvard: Australian Style

Messad, S & Bensmail, S & Salhi, O & Djouahra Fahem, D 2022, 'Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety', European Journal of Biology, vol. 81, no. 1, pp. 58-67, viewed 4 May. 2024, https://doi.org/10.26650/EurJBiol.2022.1109068


Harvard: Author-Date Style

Messad, S. and Bensmail, S. and Salhi, O. and Djouahra Fahem, D. (2022) ‘Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety’, European Journal of Biology, 81(1), pp. 58-67. https://doi.org/10.26650/EurJBiol.2022.1109068 (4 May. 2024).


MLA

Messad, Sara, and Souhila Bensmail and Omar Salhi and Djamila Djouahra Fahem. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety.” European Journal of Biology, vol. 81, no. 1, 2022, pp. 58-67. [Database Container], https://doi.org/10.26650/EurJBiol.2022.1109068


Vancouver

Messad S, Bensmail S, Salhi O, Djouahra Fahem D. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety. European Journal of Biology [Internet]. 4 May. 2024 [cited 4 May. 2024];81(1):58-67. Available from: https://doi.org/10.26650/EurJBiol.2022.1109068 doi: 10.26650/EurJBiol.2022.1109068


ISNAD

Messad, Sara - Bensmail, Souhila - Salhi, Omar - Djouahra Fahem, Djamila. Effect of Extraction Method on Organoleptic, Physicochemical Properties and Some Biological Activities of Olive Oil from the Algerian Chemlal Variety”. European Journal of Biology 81/1 (May. 2024): 58-67. https://doi.org/10.26650/EurJBiol.2022.1109068



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Submitted27.04.2022
Accepted23.05.2022
Published Online16.06.2022

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