Research Article


DOI :10.26650/EurJBiol.2020.0088   IUP :10.26650/EurJBiol.2020.0088    Full Text (PDF)

Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats

Savaş Üstünovaİsmail MeralAbdürrahim KoçyiğitAysu KılıçÖykü ZeybekMehmet ÜyüklüSedat Meydan

Objective: This study was designed for investigating the effectiveness of the Nigella sativa (N. sativa) extract on apoptosis in cerebrum and hippocampus and also plasma oxidative stress, in pentylenetetrazol (PTZ)-induced kindling rats. Materials and Methods: The kindling model was induced by subconvulsive doses (35 mg/kg i.p.) of intraperitoneal PTZ injections in N. sativa treated and non-treated PTZ groups (PTZ and PTZ + NS). The PTZ + NS group were also treated with an extract of N. sativa (10 mg/kg) 2 h before each PTZ injection. The total oxidant and antioxidant status, oxidative stress index, paraoxonase, arylesterase, catalase (CAT) and total thiol levels were analyzed in plasma. Brain derived neurotrophic factor (BDNF), cyclin-B1 and B-cell lymphoma 2 (Bcl-2) expressions were investigated in the cerebrum and hippocampus. Results: PTZ decreased the oxidative stress by increasing the activities of CAT, arylesterase, and paraoxonase. N. sativa decreased activities of arylesterase, paraoxonase, while increasing the CAT. It also brought the decreased BDNF and Bcl-2 expression levels to their normal levels in the cerebrum but not in the hippocampus. Conclusion: N. sativa treatment improved the PTZ induced-impairments in BDNF and Bcl-2 expressions, resulting in a neuronal apoptosis in the cerebrum, without affecting blood oxidative stress.


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APA

Üstünova, S., Meral, İ., Koçyiğit, A., Kılıç, A., Zeybek, Ö., Üyüklü, M., & Meydan, S. (2020). Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats. European Journal of Biology, 79(2), 124-131. https://doi.org/10.26650/EurJBiol.2020.0088


AMA

Üstünova S, Meral İ, Koçyiğit A, Kılıç A, Zeybek Ö, Üyüklü M, Meydan S. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats. European Journal of Biology. 2020;79(2):124-131. https://doi.org/10.26650/EurJBiol.2020.0088


ABNT

Üstünova, S.; Meral, İ.; Koçyiğit, A.; Kılıç, A.; Zeybek, Ö.; Üyüklü, M.; Meydan, S. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats. European Journal of Biology, [Publisher Location], v. 79, n. 2, p. 124-131, 2020.


Chicago: Author-Date Style

Üstünova, Savaş, and İsmail Meral and Abdürrahim Koçyiğit and Aysu Kılıç and Öykü Zeybek and Mehmet Üyüklü and Sedat Meydan. 2020. “Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats.” European Journal of Biology 79, no. 2: 124-131. https://doi.org/10.26650/EurJBiol.2020.0088


Chicago: Humanities Style

Üstünova, Savaş, and İsmail Meral and Abdürrahim Koçyiğit and Aysu Kılıç and Öykü Zeybek and Mehmet Üyüklü and Sedat Meydan. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats.” European Journal of Biology 79, no. 2 (May. 2024): 124-131. https://doi.org/10.26650/EurJBiol.2020.0088


Harvard: Australian Style

Üstünova, S & Meral, İ & Koçyiğit, A & Kılıç, A & Zeybek, Ö & Üyüklü, M & Meydan, S 2020, 'Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats', European Journal of Biology, vol. 79, no. 2, pp. 124-131, viewed 11 May. 2024, https://doi.org/10.26650/EurJBiol.2020.0088


Harvard: Author-Date Style

Üstünova, S. and Meral, İ. and Koçyiğit, A. and Kılıç, A. and Zeybek, Ö. and Üyüklü, M. and Meydan, S. (2020) ‘Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats’, European Journal of Biology, 79(2), pp. 124-131. https://doi.org/10.26650/EurJBiol.2020.0088 (11 May. 2024).


MLA

Üstünova, Savaş, and İsmail Meral and Abdürrahim Koçyiğit and Aysu Kılıç and Öykü Zeybek and Mehmet Üyüklü and Sedat Meydan. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats.” European Journal of Biology, vol. 79, no. 2, 2020, pp. 124-131. [Database Container], https://doi.org/10.26650/EurJBiol.2020.0088


Vancouver

Üstünova S, Meral İ, Koçyiğit A, Kılıç A, Zeybek Ö, Üyüklü M, Meydan S. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats. European Journal of Biology [Internet]. 11 May. 2024 [cited 11 May. 2024];79(2):124-131. Available from: https://doi.org/10.26650/EurJBiol.2020.0088 doi: 10.26650/EurJBiol.2020.0088


ISNAD

Üstünova, Savaş - Meral, İsmail - Koçyiğit, Abdürrahim - Kılıç, Aysu - Zeybek, Öykü - Üyüklü, Mehmet - Meydan, Sedat. Effects of Nigella sativa on Plasma Oxidative Stress, and Some Apoptotic Protein Markers in Cerebrum and Hippocampus in Pentylenetetrazol-Induced Kindling Rats”. European Journal of Biology 79/2 (May. 2024): 124-131. https://doi.org/10.26650/EurJBiol.2020.0088



TIMELINE


Submitted13.10.2020
Accepted12.11.2020
Published Online21.12.2020

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