Research Article


DOI :10.26650/EurJBiol.2024.1526736   IUP :10.26650/EurJBiol.2024.1526736    Full Text (PDF)

Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D

Soukaina ImlilssZahira AhmadiGhita JekkiElif Eren

Objective: Overcoming resistance to apoptosis is one of the main goals of cancer treatment. Subversion of apoptosis to pyroptosis, an inflammatory cell death mediated by Gasdermin family members, was previously proposed as an alternative strategy for killing cancer cells. Since the pyroptotic activity of Gasdermin D has been mainly studied in the context of inflammasome activation and its association with cancer is mostly based on expression correlation, we sought to determine whether Gasdermin D-mediated pyroptosis could be triggered in response to drugs used to induce apoptosis in cancer cells.

Materials and Methods: Cancer cells were treated with different concentrations of etoposide, gemcitabine, and cisplatin, which are used as chemotherapeutic agents in various ongoing clinical trials. Membrane integrity was evaluated by measuring lactate dehydrogenase release, and Gasdermin D and Caspase activations and subcellular localisation of Gasdermin D were determined by western blotting.

Results: Upon treatment with the well-known apoptosis inducer etoposide, a necrotic form of programmed cell death was observed in different cancer cell lines even at low drug concentrations. Additionally, cleavage analysis revealed a 20-kDa-Gasdermin D fragment in the supernatant of treated cells. Surprisingly, this cleaved form was localised in the nuclei of etoposide-treated cells rather than in the well-defined cytosolic and plasma membrane localisations.

Conclusion: Overall, these results demonstrate the complex interplay between different cell death pathways and suggest that Gasdermin D generates a fragment presenting an unknown function in the nuclei of etoposide-treated cancer cell lines. 


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APA

Imlilss, S., Ahmadi, Z., Jekki, G., & Eren, E. (2024). Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D. European Journal of Biology, 83(2), 204-212. https://doi.org/10.26650/EurJBiol.2024.1526736


AMA

Imlilss S, Ahmadi Z, Jekki G, Eren E. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D. European Journal of Biology. 2024;83(2):204-212. https://doi.org/10.26650/EurJBiol.2024.1526736


ABNT

Imlilss, S.; Ahmadi, Z.; Jekki, G.; Eren, E. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D. European Journal of Biology, [Publisher Location], v. 83, n. 2, p. 204-212, 2024.


Chicago: Author-Date Style

Imlilss, Soukaina, and Zahira Ahmadi and Ghita Jekki and Elif Eren. 2024. “Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D.” European Journal of Biology 83, no. 2: 204-212. https://doi.org/10.26650/EurJBiol.2024.1526736


Chicago: Humanities Style

Imlilss, Soukaina, and Zahira Ahmadi and Ghita Jekki and Elif Eren. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D.” European Journal of Biology 83, no. 2 (Dec. 2024): 204-212. https://doi.org/10.26650/EurJBiol.2024.1526736


Harvard: Australian Style

Imlilss, S & Ahmadi, Z & Jekki, G & Eren, E 2024, 'Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D', European Journal of Biology, vol. 83, no. 2, pp. 204-212, viewed 22 Dec. 2024, https://doi.org/10.26650/EurJBiol.2024.1526736


Harvard: Author-Date Style

Imlilss, S. and Ahmadi, Z. and Jekki, G. and Eren, E. (2024) ‘Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D’, European Journal of Biology, 83(2), pp. 204-212. https://doi.org/10.26650/EurJBiol.2024.1526736 (22 Dec. 2024).


MLA

Imlilss, Soukaina, and Zahira Ahmadi and Ghita Jekki and Elif Eren. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D.” European Journal of Biology, vol. 83, no. 2, 2024, pp. 204-212. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1526736


Vancouver

Imlilss S, Ahmadi Z, Jekki G, Eren E. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D. European Journal of Biology [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];83(2):204-212. Available from: https://doi.org/10.26650/EurJBiol.2024.1526736 doi: 10.26650/EurJBiol.2024.1526736


ISNAD

Imlilss, Soukaina - Ahmadi, Zahira - Jekki, Ghita - Eren, Elif. Etoposide Treatment of Cancer Cell Lines Results in Nuclear Localisation of Cleaved Gasdermin D”. European Journal of Biology 83/2 (Dec. 2024): 204-212. https://doi.org/10.26650/EurJBiol.2024.1526736



TIMELINE


Submitted02.08.2024
Accepted01.11.2024
Published Online19.12.2024

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