Research Article


DOI :10.26650/EurJBiol.2020.0040   IUP :10.26650/EurJBiol.2020.0040    Full Text (PDF)

Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae)

Damla Amutkan MutluIrmak PolatZekiye Suludere

Objective: The alimentary canal is composed of the foregut, the midgut, and the hindgut in insects. The hindgut is the region where the reabsorption of the water and some ions occur and the feces are generated. The hindgut is generally divided into 3 parts as the ileum, the colon and the rectum in insects. The morphological and fine structures of these parts can be used as the taxonomical character. The aim of this study is to investigate the fine structure and morphology of the ileum in the last instar nymph of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae). Materials and Methods: In this study, morphology, histology, and ultrastructure of the ileum in the last instar nymphal C. fuscus fuscus were examined by stereomicroscope, light microscope, scanning electron microscope, and transmission electron microscope. Results: Ileum is a tube-like structure, which is situated between the midgut and the colon. The wall of the ileum is composed of muscle tissue, connective tissue, epithelial tissue, and cuticular intima from the outermost to the innermost. Those singlelayered epithelial cells have globular nuclei, and some granulated endoplasmic reticulum can be observed in photographs. Besides, the regenerative cells groups called regenerative nidi are clear in some basal regions of the epithelial layer. Conclusion: The general morphology and structure of the nymphal ileum in C. fuscus fuscus have some differences with the insects belonging to Orthoptera orders, but its histological, cytological, and fine structure are very alike.


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References

  • 1. Lee WY, Chen ME, Lin TL. Morphology and ultrastructure of the alimentary canal of oriental fruit fly Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (I): the structure of the foregut and cardia. Zool Stud 1998; 1(37): 95-101. google scholar
  • 2. Levy SM, Falleiros ÂM, Moscardi F, Gregório EA, Toledo LA. Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). Neotrop Entomol 2004; 33(4): 427-31. google scholar
  • 3. Levy SM, Falleiros ÂM, Moscardi F, Gregório EA, Toledo LA. Ultramorphology of digestive tract of Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) at final larval development. Semina: Ciências Agrárias 2008; 29(2): 313-21. google scholar
  • 4. Bution ML, Caetano FH, Britto FB, Gomes GT, Zara FJ. Histology and histochemistry of the ventriculus of Dolichoderus (=Monacis) bispinosus (OLIVIER, 1792) (Hymenoptera: Formicidae). Micron 2006; 37(3): 249-54. google scholar
  • 5. Boonsriwong W, Sukontason K, Olson JK, Vogtsberger RC, Chaithong U, Kuntalue B, et. al. Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae). Parasitol Res 2007; 100(3): 561-74. google scholar
  • 6. Rubio G, José D, Bustillo P, Alex E, Vallejo E, Luis F, et. al. Alimentary canal and reproductive tract of Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae, Scolytinae). Neotrop Entomol 2008; 37(2): 143-51. google scholar
  • 7. Serrão JE, Ronnau M, Neves CA, Campos LA, Zanuncio JC. Ultrastructure of anterior midgut region of corbiculate bees (Hymenoptera: Apidae). Ann Entomol Soc Am 2008; 101(5): 915-21. google scholar
  • 8. Fialho MD, Zanuncio JC, Neves CA, Ramalho FS, Serrão JE. Ultrastructure of the digestive cells in the midgut of the predator Brontocoris tabidus (Heteroptera: Pentatomidae) after different feeding periods on prey and plants. Ann Entomol Soc Am 2009; 102(1): 119-27. google scholar
  • 9. Santos CG, Neves CA, Zanuncio JC, Serrão JE. Postembryonic development of rectal pads in bees (Hymenoptera, Apidae). Anat Rec 2009; 292(10): 1602-11. google scholar
  • 10. De Sousa G, Scudeler EL, Abrahão J, Conte H. Functional morphology of the crop and proventriculus of Sitophilus zeamais (Coleoptera: Curculionidae). Ann Entomol Soc Am 2013; 106(6): 846-52. google scholar
  • 11. Gonçalves WG, Fernandes KM, Barcellos MS, Silva FP, Magalhaes-Junior MJ, Zanuncio JC, et. al. Ultrastructure and immunofluorescence of the midgut of Bombus morio (Hymenoptera: Apidae: Bombini). Comptes Rendus Biologies 2014; 337(6): 365-72. google scholar
  • 12. Polat I, Suludere S, Candan S. Histological and ultrastructural features of the rectum in Poecilimon cervus Karabağ, 1950 (Orthoptera: Tettigoniidae). Microsc Res Techniq 2017; 80: 195-201. google scholar
  • 13. Harris MN, Azman S, Nurul Wahida O. Gross anatomy and histology of alimentary system of stick insect, Pylaemenes mitratus (Phasmid: Basillidae). Serangga 2019; 24(1): 151-8. google scholar
  • 14. Wigglesworth VB. The Principles of Insect Physiology. 7th ed. London: Chapman and Hall; 1972. google scholar
  • 15. Nation JL. Insect Physiology and Biochemistry. CRC Press Boca Raton FL; 2002. google scholar
  • 16. Bution ML, Caetano FH. Ileum of the Cephalotes ants: A specialized structure to harbor symbionts microorganisms. Micron 2008; 39: 897-909. google scholar
  • 17. Çakıcı Ö. Histological and Ultrastructural Investigations on the Digestive System of Melanogryllus desertus Pallas (Orthoptera: Gryllidae). E.Ü. Fen Bilimleri Enstitüsü, Doktora Tezi. 2008. google scholar
  • 18. Çakıcı Ö, Ergen G. Histological and ultrastructural investigations on hindgut of Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae). German J Zool Res 2013; 1(1): 1-6. google scholar
  • 19. Beams HW, Tahmisian TN, Devine RL. Electron microscope studies on the cells of the Malpighian tubules of the grasshopper (Orthoptera, Acrididae). J. Biophysic Biochem Cytol 1955; 1(3): 197-214. google scholar
  • 20. Peacock AJ. Ultrastructure of the ileum of Locusta migratoria. J Morphol 1986; 188: 191-201. google scholar
  • 21. Irvine B, Audsley N, Lechleitner R, Meredith J, Thomson B, Phillips J. Transport properties of locust ileum in vitro: effects of cyclic AMP. J Exp Biol 1988; 137: 361-85. google scholar
  • 22. Prado MA, Montuenga LM, Villaro AC, Etayo JC, Polak JM, Sesma MP. A novel granular cell type of locust Malpighian tubules: ultrastructural and immunocytochemical study. Cell Tissue Res 1992; 268(1): 123-30. google scholar
  • 23. Gaino E, Rebora M. The duct connecting Malpighian tubules and gut: an ultrastructural and comparative analysis in various Ephemeroptera nymphs (Pterygota). Zoomorphol 2000; 120(2): 99-106. google scholar
  • 24. Acar G. Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae)’ta Malpighi Tüpçüklerinin Morfoloji ve Histolojisi. E.Ü. Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi. 2009. google scholar
  • 25. Conti B, Berti F, Mercati D, Giusti F, Dallai R. The ultrastructure of Malpighian tubules and the chemical composition of the cocoon of Aeolothrips intermedius Bagnall (Thysanoptera). J Morphol 2010; 271(2): 244-54. google scholar
  • 26. De Azeredo-Oliveira MT, da Silva TL, Mello ML. Mg2+-dependent ATPase activity in Malpighian tubules of Triatoma infestans Klug. Micron 2012; 43(2-3): 298-304. google scholar
  • 27. Ferreira RA, Zacarin EC, Malaspina O, Bueno OC, Tomotake ME, Pereira AM. Cellular responses in the Malpighian tubules of Scaptotrigona postica (Latreille, 1807) exposed to low doses of fipronil and boric acid. Micron 2013; 46: 57-65. google scholar
  • 28. Pal R, Kumar K. Malpighian tubules of adult flesh fly, Sarcophaga ruficornis Fab. (Diptera: Sarcophagidae): an ultrastructural study. Tissue Cell 2013; 45(5): 312-7. google scholar
  • 29. Poolprasert P, Mongkolchaichana E, Senarat S, Kettratad J, Yenchum W, Angsirijinda W. Light microscopic observations of the mesentero-proctodeal regions in adult Catopsilla pomona (Fabricius, 1758) (Lepidoptera: Pieridae). Suranaree J Sci Technol 2015; 22(1): 99-103. google scholar
  • 30. Giglio A, Perrotta ID, Brandmayr P. Exosomes: Ultrastructural evidence in epithelial cells of Malpighian tubules. Micron 2017; 100: 34-7. google scholar
  • 31. Rivera-Vega L, Mikó I. Know your insect: Malpighian tubules in Trichoplusia ni (Lepidoptera: Noctuidae). Res Ideas Outcomes. 2017 Feb 01. doi: 10.3897/rio.3.e11827 e11827. google scholar
  • 32. Gullan PJ, Cranston PS, editors. İç anatomi ve fizoloji. Böcekler: entomolojinin ana hatları. Ankara: Nobel; 2012. google scholar
  • 33. Ragge DR. Grasshoppers, Crickets and Cockroaches of the British Isles. London: F Warne and Co; 1965. google scholar
  • 34. Sadiq S, Panhwar WA, Sultana R, Saeed M, Wagan SA, Ahmed S. New record of Conocephalus (Anisoptera) fuscus (Fabricius, 1793) (Conocephalinae: Tettigoniidae: Orthoptera) from Pakistan. J Entomol Zool Stud 2017; 5(3): 1431-4. google scholar
  • 35. Polat I. The ultrastructural features of the digestive, excretory, female and male reproductive systems of Poecilimon cervus Karabag, 1950. G.Ü. Fen Bilimleri Enstitüsü, Doktora Tezi. 2016. google scholar
  • 36. Villaro AC, Garayoa M, Lezaun MJ, Sesma P. Light and electron microscopic study of the hindgut of the ant (Formica nigricans, Hymenoptera): I. structure of the ileum. J Morphol 1999; 242(3): 189-204. google scholar
  • 37. Lopez-Guerrero Y. Anatomy and histology of the digestive system of Cephalodesmius armiger Westwood (Coleoptera, Scarabaeidae, Scarabaeinae). Coleop Bull 2002; 56(1): 97-107. google scholar
  • 38. Amutkan D, Suludere Z, Candan S. Ultrastructure of digestive canal of Graphosoma lineatum (Linnaeus, 1758) (Heteroptera: Pentatomidae). J Entomol Res Soc 2015; 17(3): 75-96. google scholar
  • 39. Jarial MS; Scudder GGE. The morphology and ultrastructure of the Malpighian tubules and hindgut in Cenocorixa bifida (Hung.) (Hemiptera, Corixidae). Z Morphol Oekol Tiere 1970; 68(4): 269-99. google scholar
  • 40. Chapman RF. The Insects: Structure and Function. 5th ed. New York: Cambridge University Press; 2013. google scholar
  • 41. Klowden MJ. Physiological Systems in Insects. 3rd ed. London: Academic Press; 2013. google scholar
  • 42. Ulrich RG, Buthala DA, Klug MJ. Microbiota associated with the gastrointestinal tract of the common house cricket, Acheta domestica. Appl Environ Microbiol 1981; 41(1): 246-54. google scholar
  • 43. Woodring J, Lorenz MW. Feeding, nutrient flow, and functional gut morphology in the cricket Gryllus bimaculatus. J Morphol 2007; 268: 815-25. google scholar
  • 44. Illa-Bochaca I, Montuenga LM. The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells. J Exp Biol 2006; 209: 2215-23. google scholar
  • 45. Park MS, Park P, Takeda M. Starvation induces apoptosis in the midgut nidi of Periplaneta americana: a histochemical and ultrastructural study. Cell Tissue Res 2009; 335: 631-8. google scholar
  • 46. Çakıcı Ö, Ergen G. Histologic description of midgut in Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae). Biharean Biol 2012; 6(2): 108-111. google scholar
  • 47. Nation JL. Insect Physiology and Biochemistry. 2nd ed. Florida: CRC Press; 2008. google scholar
  • 48. Gonçalves WG, Fernandes KM, Santana WC, Martins GF, Zanuncio JC, Serrão JE. Post-embryonic changes in the hindgut of honeybee Apis mellifera workers: Morphology, cuticle deposition, apoptosis, and cell proliferation. Dev Biol 2017; 431(2): 194-204. google scholar

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APA

Amutkan Mutlu, D., Polat, I., & Suludere, Z. (2020). Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae). European Journal of Biology, 79(1), 7-13. https://doi.org/10.26650/EurJBiol.2020.0040


AMA

Amutkan Mutlu D, Polat I, Suludere Z. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae). European Journal of Biology. 2020;79(1):7-13. https://doi.org/10.26650/EurJBiol.2020.0040


ABNT

Amutkan Mutlu, D.; Polat, I.; Suludere, Z. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae). European Journal of Biology, [Publisher Location], v. 79, n. 1, p. 7-13, 2020.


Chicago: Author-Date Style

Amutkan Mutlu, Damla, and Irmak Polat and Zekiye Suludere. 2020. “Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae).” European Journal of Biology 79, no. 1: 7-13. https://doi.org/10.26650/EurJBiol.2020.0040


Chicago: Humanities Style

Amutkan Mutlu, Damla, and Irmak Polat and Zekiye Suludere. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae).” European Journal of Biology 79, no. 1 (Nov. 2024): 7-13. https://doi.org/10.26650/EurJBiol.2020.0040


Harvard: Australian Style

Amutkan Mutlu, D & Polat, I & Suludere, Z 2020, 'Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae)', European Journal of Biology, vol. 79, no. 1, pp. 7-13, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2020.0040


Harvard: Author-Date Style

Amutkan Mutlu, D. and Polat, I. and Suludere, Z. (2020) ‘Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae)’, European Journal of Biology, 79(1), pp. 7-13. https://doi.org/10.26650/EurJBiol.2020.0040 (22 Nov. 2024).


MLA

Amutkan Mutlu, Damla, and Irmak Polat and Zekiye Suludere. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae).” European Journal of Biology, vol. 79, no. 1, 2020, pp. 7-13. [Database Container], https://doi.org/10.26650/EurJBiol.2020.0040


Vancouver

Amutkan Mutlu D, Polat I, Suludere Z. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae). European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];79(1):7-13. Available from: https://doi.org/10.26650/EurJBiol.2020.0040 doi: 10.26650/EurJBiol.2020.0040


ISNAD

Amutkan Mutlu, Damla - Polat, Irmak - Suludere, Zekiye. Gross Morphology, Histology and Ultrastructure of the Nymphal Ileum of Conocephalus (Xiphidion) fuscus fuscus (Fabricius, 1793) (Orthoptera, Tettigoniidae)”. European Journal of Biology 79/1 (Nov. 2024): 7-13. https://doi.org/10.26650/EurJBiol.2020.0040



TIMELINE


Submitted08.11.2019
Accepted30.01.2020
Published Online17.06.2020

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