European Journal of Biology

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Research Article


DOI :10.26650/EurJBiol.2019.0027   IUP :10.26650/EurJBiol.2019.0027    Full Text (PDF)

Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758)

Sudeshna Sarker, Thangapalam Jawahar Abraham

Objective: This study assessed the pathogenicity of Flavobacterium columnare isolated from the gill-rot of catfish, Clarias batrachus in West Bengal, India. Materials and Methods: The diseased catfish were examined as per standard laboratory practices. F. columnare SGM4 was identified based on the morphological, phenotypic and genotypic characterization. Abrasion-immersion and agar-disc diffusion methods were followed to assess the pathogenicity and antibiotic sensitivity of F. columnare, respectively. Results: The diseased catfish had white gills, tail rot, body discolouration, saddle-back, peeled skin, emaciation, and inflamed kidney. The yellow-pigmented rhizoid colonies from the gills of catfish were identified as F. columnare. Phylogenetically, F. columnare SGM4 branched with F. columnare strains. In abrasion-immersion challenge experiments, F. columnare SGM4 induced considerable mortalities (45%) in C. batrachus at 7.2 x 10⁶ cells/mL at 24-30 °C. In challenged catfish, it caused cutaneous lesions, tail rot, white patches on the gills and degeneration of internal organs. F. columnare strains were highly sensitive to broad-spectrum antibiotics except for sulphafurazole. Conclusion: Adoption of good nursery practices and appropriate health management measures would help to minimize the development and spread of columnaris disease.

Keywords: Clarias batrachus, Gill-rot, Flavobacterium columnare, Pathogenicity, Antibiotic susceptibility

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APA

Sarker, S., & Abraham, T.J. (2019). Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758). European Journal of Biology, 78(2), 139-146. https://doi.org/10.26650/EurJBiol.2019.0027


AMA

Sarker S, Abraham T J. Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758). European Journal of Biology. 2019;78(2):139-146. https://doi.org/10.26650/EurJBiol.2019.0027


ABNT

Sarker, S.; Abraham, T.J. Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758). European Journal of Biology, [Publisher Location], v. 78, n. 2, p. 139-146, 2019.


Chicago: Author-Date Style

Sarker, Sudeshna, and Thangapalam Jawahar Abraham. 2019. “Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758).” European Journal of Biology 78, no. 2: 139-146. https://doi.org/10.26650/EurJBiol.2019.0027


Chicago: Humanities Style

Sarker, Sudeshna, and Thangapalam Jawahar Abraham. “Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758).” European Journal of Biology 78, no. 2 (Nov. 2024): 139-146. https://doi.org/10.26650/EurJBiol.2019.0027


Harvard: Australian Style

Sarker, S & Abraham, TJ 2019, 'Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758)', European Journal of Biology, vol. 78, no. 2, pp. 139-146, viewed 26 Nov. 2024, https://doi.org/10.26650/EurJBiol.2019.0027


Harvard: Author-Date Style

Sarker, S. and Abraham, T.J. (2019) ‘Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758)’, European Journal of Biology, 78(2), pp. 139-146. https://doi.org/10.26650/EurJBiol.2019.0027 (26 Nov. 2024).


MLA

Sarker, Sudeshna, and Thangapalam Jawahar Abraham. “Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758).” European Journal of Biology, vol. 78, no. 2, 2019, pp. 139-146. [Database Container], https://doi.org/10.26650/EurJBiol.2019.0027


Vancouver

Sarker S, Abraham TJ. Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758). European Journal of Biology [Internet]. 26 Nov. 2024 [cited 26 Nov. 2024];78(2):139-146. Available from: https://doi.org/10.26650/EurJBiol.2019.0027 doi: 10.26650/EurJBiol.2019.0027


ISNAD

Sarker, Sudeshna - Abraham, ThangapalamJawahar. “Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758)”. European Journal of Biology 78/2 (Nov. 2024): 139-146. https://doi.org/10.26650/EurJBiol.2019.0027



TIMELINE


Submitted17.09.2019
Accepted21.11.2019
Published Online06.12.2019

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