Leptin Enhances Nitric Oxide Production and Decreases  Blood Flow in Rat Skeletal Muscle

Üstünova Savaş; Kapucu Ayşegül; Demirci Tansel Cihan

doi:https://dx.doi.org/10.26650/EurJBiol.2022.1082814

Research Article

DOI :10.26650/EurJBiol.2022.1082814 IUP :10.26650/EurJBiol.2022.1082814 Full Text (PDF)

Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle

Savaş Üstünova, Ayşegül Kapucu, Cihan Demirci Tansel

Objective: There are a few studies related to nitric oxide (NO) and leptin interaction in the regulation of physiological events in skeletal muscle. Therefore, the aim of the present study is to investigate the interaction of leptin and NO in response to blood flow and nitric oxide synthase (NOS) distribution on rat skeletal muscle. Materials and Methods: Twenty-four adult-male Wistar albino rats were divided into 4 groups: control (C), Leptin (LP) (50 μg/kg), L-NG-nitroarginine methyl ester (LN) (a non-specific nitric oxide synthase inhibitor, 10 mg/kg,) and L-NAME+Leptin (LN+LP) administrated groups. Drugs were administered via the right jugular vein. Hemodynamic parameters: mean arterial pressure, heart rate and blood flow were recorded during the experiment and at the end blood samples for biochemical analyses of leptin and nitrite/nitrate levels and gastrocnemius muscle tissues from the right hindlimb for NOS distribution were taken. Results: Leptin infusion after L-NAME administration significantly decreased heart rate and blood flow (p<0.05, p<0.001). In addition, there was no change in the mean arterial pressure in the leptin group and leptin-administered L-NAME group. Leptin levels were the highest in the LP+LN group (p<0.01). Decreased nitrate/nitrate levels with L-NAME administration (p<0.01 vs C) reached control values by leptin infusion. Both endothelial NOS (eNOS) and neuronal NOS (nNOS) distributions were observed in the skeletal muscle cells of the leptin group. Conclusion: Although NO synthesis is inhibited by L-NAME, it is concluded that leptin partially enhances NO production and leptin uses NO as a mediator in its physiological effects.

Keywords: Blood flow, leptin, nitric oxide, rat; skeletal muscle

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APA

Üstünova, S., Kapucu, A., & Demirci Tansel, C. (2022). Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle. European Journal of Biology, 81(1), 18-25. https://doi.org/10.26650/EurJBiol.2022.1082814

AMA

Üstünova S, Kapucu A, Demirci Tansel C. Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle. European Journal of Biology. 2022;81(1):18-25. https://doi.org/10.26650/EurJBiol.2022.1082814

ABNT

Üstünova, S.; Kapucu, A.; Demirci Tansel, C. Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle. European Journal of Biology, [Publisher Location], v. 81, n. 1, p. 18-25, 2022.

Chicago: Author-Date Style

Üstünova, Savaş, and Ayşegül Kapucu and Cihan Demirci Tansel. 2022. “Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle.” European Journal of Biology 81, no. 1: 18-25. https://doi.org/10.26650/EurJBiol.2022.1082814

Chicago: Humanities Style

Üstünova, Savaş, and Ayşegül Kapucu and Cihan Demirci Tansel. “Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle.” European Journal of Biology 81, no. 1 (May. 2024): 18-25. https://doi.org/10.26650/EurJBiol.2022.1082814

Harvard: Australian Style

Üstünova, S & Kapucu, A & Demirci Tansel, C 2022, 'Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle', European Journal of Biology, vol. 81, no. 1, pp. 18-25, viewed 4 May. 2024, https://doi.org/10.26650/EurJBiol.2022.1082814

Harvard: Author-Date Style

Üstünova, S. and Kapucu, A. and Demirci Tansel, C. (2022) ‘Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle’, European Journal of Biology, 81(1), pp. 18-25. https://doi.org/10.26650/EurJBiol.2022.1082814 (4 May. 2024).

MLA

Üstünova, Savaş, and Ayşegül Kapucu and Cihan Demirci Tansel. “Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle.” European Journal of Biology, vol. 81, no. 1, 2022, pp. 18-25. [Database Container], https://doi.org/10.26650/EurJBiol.2022.1082814

Vancouver

Üstünova S, Kapucu A, Demirci Tansel C. Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle. European Journal of Biology [Internet]. 4 May. 2024 [cited 4 May. 2024];81(1):18-25. Available from: https://doi.org/10.26650/EurJBiol.2022.1082814 doi: 10.26650/EurJBiol.2022.1082814

ISNAD

Üstünova, Savaş - Kapucu, Ayşegül - Demirci Tansel, Cihan. “Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle”. European Journal of Biology 81/1 (May. 2024): 18-25. https://doi.org/10.26650/EurJBiol.2022.1082814

Volume 81, Issue 12022, P. 18-25

TIMELINE

Submitted	04.03.2022
Accepted	04.04.2022
Published Online	29.04.2022

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