Research Article


DOI :10.26650/EurJBiol.2023.1313209   IUP :10.26650/EurJBiol.2023.1313209    Full Text (PDF)

Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry

Mete Bora TüzünerBurçin Alev Tüzüner

Objective: Breast cancer is the commonest and the deadliest malignancy among women. Iron is known as an essential element for cell growth and division. The connection between oxidative stress, antioxidants, and progression, aggressiveness, and recurrence of breast cancer, is widely recognized to involve impaired iron metabolism. The purpose of this study was to investigate the connection between the tumor characteristics and the levels of total iron in breast tissues by employing state of the art technology, inductively-coupled plasma/mass spectrometry (ICP-MS) with our in-house analysis methodology for tissue samples.

Materials and Methods: Iron contents were determined in 25 tissue sets (matched tumor and peritumoral tissues) collected from 25 women diagnosed with invasive ductal breast cancer. In addition, three cancer-free breast tissues, obtained from breast reduction surgery, were analyzed as a normal group. Collected samples were digested and introduced to ICP-MS for iron analysis.

Results: Our method showed a low rate of measurement error (<10%). A highly significant (p<0.001) ∼3 fold difference of iron concentrations were observed in tumors (24.73±6.15 ppb/mg) as compared to peritumoral tissues (9.10±4.84 ppb/mg). As the grade and stage of the cancer increases, iron levels in tumor tissues were also found to be increased (p=0.006, p=0.022 respectively).

Conclusion: Our ICP-MS based method can be reliably performed at the established conditions for tissue specimens, and also have potential to be used in clinical practice. Understanding the relationship between tissue iron levels and tumor characteristics is essential in identifying potential prevention and treatment strategies. 

Keywords: IronBreast cancerROSICP-MS

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APA

Tüzüner, M.B., & Tüzüner, B.A. (2023). Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry. European Journal of Biology, 82(2), 270-279. https://doi.org/10.26650/EurJBiol.2023.1313209


AMA

Tüzüner M B, Tüzüner B A. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry. European Journal of Biology. 2023;82(2):270-279. https://doi.org/10.26650/EurJBiol.2023.1313209


ABNT

Tüzüner, M.B.; Tüzüner, B.A. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 270-279, 2023.


Chicago: Author-Date Style

Tüzüner, Mete Bora, and Burçin Alev Tüzüner. 2023. “Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry.” European Journal of Biology 82, no. 2: 270-279. https://doi.org/10.26650/EurJBiol.2023.1313209


Chicago: Humanities Style

Tüzüner, Mete Bora, and Burçin Alev Tüzüner. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry.” European Journal of Biology 82, no. 2 (Jul. 2024): 270-279. https://doi.org/10.26650/EurJBiol.2023.1313209


Harvard: Australian Style

Tüzüner, MB & Tüzüner, BA 2023, 'Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry', European Journal of Biology, vol. 82, no. 2, pp. 270-279, viewed 20 Jul. 2024, https://doi.org/10.26650/EurJBiol.2023.1313209


Harvard: Author-Date Style

Tüzüner, M.B. and Tüzüner, B.A. (2023) ‘Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry’, European Journal of Biology, 82(2), pp. 270-279. https://doi.org/10.26650/EurJBiol.2023.1313209 (20 Jul. 2024).


MLA

Tüzüner, Mete Bora, and Burçin Alev Tüzüner. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 270-279. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1313209


Vancouver

Tüzüner MB, Tüzüner BA. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry. European Journal of Biology [Internet]. 20 Jul. 2024 [cited 20 Jul. 2024];82(2):270-279. Available from: https://doi.org/10.26650/EurJBiol.2023.1313209 doi: 10.26650/EurJBiol.2023.1313209


ISNAD

Tüzüner, MeteBora - Tüzüner, BurçinAlev. Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry”. European Journal of Biology 82/2 (Jul. 2024): 270-279. https://doi.org/10.26650/EurJBiol.2023.1313209



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Submitted12.06.2023
Accepted21.07.2023
Published Online15.09.2023

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