Research Article


DOI :10.26650/EurJBiol.2019.0009   IUP :10.26650/EurJBiol.2019.0009    Full Text (PDF)

N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats

Marziyeh NaimiMehrdad ShariatiSyrus NaimiMohammad Amin Edalatmanesh

Objective: The purpose of this study was to evaluate the protective effect of N-acetylcystein (NAC) on ovarian tissue and serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol, and progesterone in Acrylamide (AA)treated adult rats. Materials and Methods: Forty-two adult female Wistar rats were randomly assigned into 7 groups of 6 including: a control group without treatment, a placebo group received distilled water intraperitoneally, an AA group received 50 mg/kg by oral gavage, a NAC group received 40 mg/kg intraperitoneally, an AA+NAC10, AA+NAC20 and an AA+NAC40 groups received 10, 20 and 40 mg/kg of NAC intraperitoneally, respectively and then also received 50 mg/kg AA by oral gavage for 28 days. Serum levels of FSH, LH, estradiol and progesterone were measured by radioimmunoassay method and ovarian tissue was evaluated histopathologically. Results: Administrating AA alone decreased the number of ovarian follicles, corpus luteum and the levels of FSH, estradiol and progesterone, while increased the number of atretic follicles and LH level compared to the control, placebo and NAC groups (p<0.05). The administration of NAC alone had no effect on the number of ovarian follicles, corpus luteum and the level of hormones compared to the control and placebo groups (p>0.05). Following AA+NAC20 and AA+NAC40 administration and not AA+NAC10, the number of ovarian follicles, corpus luteum and also the levels of FSH, estradiol and progesterone increased, while the number of atretic follicles and LH level decreased (p<0.05), which was in a dose-dependent manner compared to the AA group. Conclusion: NAC could recover the AA female rat reproductive toxicity in a dose-dependent manner, and improved folliculogenesis.

PDF View

References

  • 1. Rahangadale S, Jangir BL, Patil M, Verma T, Bhandarkar A, Sonkusale P, et al. Evaluation of protective effect of vitamin E on acrylamide induced testicular toxicity in wister rats. Toxicol Int 2012; 19(2): 158-61. google scholar
  • 2. Olesen PT, Olsen A, Frandsen H, Frederiksen K, Overvad K, Tjønneland A. Acrylamide exposure and incidence of breast cancer among postmenopausal women in the Danish Diet, Cancer and Health Study. Int J Cancer 2008; 122(9): 2094-100. google scholar
  • 3. Yener Y and Kalipci E. The carcinogenic effects of acrylamide formed during cooking of some foods. Academic J Cancer Res 2009; 2(1): 25-32. google scholar
  • 4. Lingnert H, Grivas S, Jagerstad M, Skog K, Tornqvist M, Aman P, et al. Acrylamide in food: mechanisms of formation and in influencing factors during heating of foods. Scand J Nutr 2002; 46(4): 159-72. google scholar
  • 5. Klaunig JE. Acrylamide carcinogenicity. J Agric Food Chem 2008; 56(15): 5984-8. google scholar
  • 6. Barber DS, Hunt JR, Ehrich MF, Lehning EJ, Lopachin RM. Metabolism, toxicokinetics and hemoglobin adduct formation in rats following subacute and subchronic acrylamide dosing. Neurotoxicology 2001; 22(3): 341-53. google scholar
  • 7. Tyl RW and Friedman MA. Effects of acrylamide on rodent reproductive performance. Reprod Toxicol 2003; 17(1): 1-13. google scholar
  • 8. Collí-Dulá RC, Friedman MA, Hansen B, Denslow ND. Transcriptomics analysis and hormonal changes of male and female neonatal rats treated chronically with a low dose of acrylamide in their drinking water. Toxicol Rep 2016; 19(3): 414-26. google scholar
  • 9. Zarei M and Shivanandappa T. Amelioration of cyclophosphamide-induced hepatotoxicity by the root extract of Decalepis hamiltonii in mice. Food Chem Toxicol 2013; 57: 179-84. google scholar
  • 10. Wei Q, Li J, Li X, Zhang L, Shi F. Reproductive toxicity in acrylamide-treated female mice. Reprod Toxicol 2014; 46: 121-8. google scholar
  • 11. ALKarim S, ElAssouli1 S, Ali S, Ayuob N, ElAssouli Z. Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity. Asian Pac J Reprod 2015; 4(3): 179–87. google scholar
  • 12. DuanX, Qiao- Wang C, Chen KL, Zhu CC, Liu J, Suna SC. Acrylamide toxic effects on mouse oocyte quality and fertility in vivo. Sci Rep 2015; 5: 11562. google scholar
  • 13. Kunle-Alabi OT, Akindele OO, Odoh MI, Oghenetega BO, Raji Y. Comparative effects of coconut water and N-Acetyl cysteine on the hypothalamo-pituitary-gonadal axis of male rats. Songklanakarin J Sci Technol 2017; 39(6): 759-64. google scholar
  • 14. Ercal N, Treeratphan P, Lutz P, Hammond TC, Matthews RH. N-acetylcysteine protects chinese hamster ovary (CHO) cells from lead-induced oxidative stress. Toxicology 1996; 108(1-2): 57- 64. google scholar
  • 15. Xue C, Liu W, Wu J, Yang X, Xu H. Chemoprotective effect of N-acetylcysteine (NAC) on cellular oxidative damages and apoptosis induced by nano titanium dioxide under UVA irradiation. Toxicol in Vitro 2011(1); 25: 110-6. google scholar
  • 16. Demedts M, Behr J, Buhl R, Costabel U, Dekhuijzen R, Jansen HM, et al. High-dose acetylcysteine in idiopathic pulmonary fibrosis. N Engl J Med. 2005; 353(21): 2229-42. google scholar
  • 17. Chiew AL, Isbister GK, Duffull SB, Buckley NA. Evidence for the changing regimens of acetylcysteine. Br J Clin Pharmacol 2016; 81(3): 471–81. google scholar
  • 18. Marcondes FK, Bianchi FJ, Tanno AP. Determination of estrous cycle phases of rats: some helpful considerations. Braz J Biol 2002; 62(4A): 609-14. google scholar
  • 19. Camacho L, Latendresse JR, Muskhelishvili L, Patton R, Bowyer JF, Thomas M, et al. Effects of acrylamide exposure on serum hormones,gene expression, cell proliferation, and histopathology in male reproductive tissues of Fischer 344 rats. Toxicol Lett 2012; 211(2): 135–43. google scholar
  • 20. Pan X, Wu X, Yan D, Peng C, Rao C, Yan H. Acrylamide-induced oxidative stress and inflammatory response are alleviated by N-acetylcysteine in PC12 cells: Involvement of the crosstalk between Nrf2 and NF-κB pathways regulated by MAPKs. Toxicol Lett 2018; 288: 55-64. google scholar
  • 21. Kojsová S, Jendeková L, Zicha J, Kunes J, Andriantsitohaina R, Pechánová O. The effect of different antioxidants on nitric oxide production in hypertensive rats. Physiol Res 2006; 55(Suppl): S316. google scholar
  • 22. Galhardo MA, Júnior CQ, Riboli Navarro PG, Morello RJ, De Jesus Simões M, De Souza Montero EF. Liver and lung late alterations following hepatic reperfusion associated to ischemic preconditioning or N-acetylcysteine. Microsurgery 2007; 27(4): 295-9. google scholar
  • 23. Usta U, Inan M, Erbas H, Aydogdu N, Puyan OF, Altaner S. Tissue damage in rat ovaries subjected to torsion and detorsion: effects of L-carnitine and N-acetyl cysteine. Pediatr Surg Int 2008; 24: 567-73. google scholar
  • 24. Danilovic A, Lucon AM, Srougi M, Shimizu MHM, Lanhez LE, Nahas WC, et al. Protective effect of N-acetylcysteine on early outcomes of deceased renal transplantation. Transplant Proc 2011; 43: 14439. google scholar
  • 25. Millea PJ. N-Acetylcysteine: Multiple clinical applications. Am Fam Physician 2009; 80: 265-9. google scholar
  • 26. Mokhtari V, Afsharian P, Shahhoseini M, Kalantar SM, Moini A. A review on various uses of n-acetyl cysteine. Cell J 2017; 19(1): 11-17. google scholar
  • 27. Altinoz E, Turkoz Y, Vardi N. The protective effect of N-acetylcysteine against acrylamide toxicity in liver and small and large intestine tissues. Bratisl Lek Listy 2015; 116(4): 252-8. google scholar
  • 28. Helal MA. The effects of N-acetyl-L-cysteine on the female reproductive performance and nephrotoxicity in rats. Ren Fail 2016; 38(2): 311-20. google scholar
  • 29. Liu J, Liu M, Ye X, Liu K, Huang J, Wang L, et al. Delay in oocyte aging in mice by the antioxidant N-acetyl-L-cysteine (NAC). Hum Reprod 2012; 27(5): 1411-20. google scholar
  • 30. Hułas-Stasiak M, Dobrowolski P, Tomaszewska E, Kostro K. Maternal acrylamide treatment reduces ovarian follicle number in newborn guinea pig offspring. Reprod Toxicol. 2013; 42: 125-31. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Naimi, M., Shariati, M., Naimi, S., & Edalatmanesh, M.A. (2019). N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats. European Journal of Biology, 78(2), 75-81. https://doi.org/10.26650/EurJBiol.2019.0009


AMA

Naimi M, Shariati M, Naimi S, Edalatmanesh M A. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats. European Journal of Biology. 2019;78(2):75-81. https://doi.org/10.26650/EurJBiol.2019.0009


ABNT

Naimi, M.; Shariati, M.; Naimi, S.; Edalatmanesh, M.A. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats. European Journal of Biology, [Publisher Location], v. 78, n. 2, p. 75-81, 2019.


Chicago: Author-Date Style

Naimi, Marziyeh, and Mehrdad Shariati and Syrus Naimi and Mohammad Amin Edalatmanesh. 2019. “N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats.” European Journal of Biology 78, no. 2: 75-81. https://doi.org/10.26650/EurJBiol.2019.0009


Chicago: Humanities Style

Naimi, Marziyeh, and Mehrdad Shariati and Syrus Naimi and Mohammad Amin Edalatmanesh. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats.” European Journal of Biology 78, no. 2 (Nov. 2024): 75-81. https://doi.org/10.26650/EurJBiol.2019.0009


Harvard: Australian Style

Naimi, M & Shariati, M & Naimi, S & Edalatmanesh, MA 2019, 'N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats', European Journal of Biology, vol. 78, no. 2, pp. 75-81, viewed 8 Nov. 2024, https://doi.org/10.26650/EurJBiol.2019.0009


Harvard: Author-Date Style

Naimi, M. and Shariati, M. and Naimi, S. and Edalatmanesh, M.A. (2019) ‘N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats’, European Journal of Biology, 78(2), pp. 75-81. https://doi.org/10.26650/EurJBiol.2019.0009 (8 Nov. 2024).


MLA

Naimi, Marziyeh, and Mehrdad Shariati and Syrus Naimi and Mohammad Amin Edalatmanesh. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats.” European Journal of Biology, vol. 78, no. 2, 2019, pp. 75-81. [Database Container], https://doi.org/10.26650/EurJBiol.2019.0009


Vancouver

Naimi M, Shariati M, Naimi S, Edalatmanesh MA. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats. European Journal of Biology [Internet]. 8 Nov. 2024 [cited 8 Nov. 2024];78(2):75-81. Available from: https://doi.org/10.26650/EurJBiol.2019.0009 doi: 10.26650/EurJBiol.2019.0009


ISNAD

Naimi, Marziyeh - Shariati, Mehrdad - Naimi, Syrus - Edalatmanesh, MohammadAmin. N-Acetylcysteine Improves Acrylamide-Induced Changes in Ovarian Tissue and Serum Levels of Pituitary-Ovarian Axis Hormones in Adult Rats”. European Journal of Biology 78/2 (Nov. 2024): 75-81. https://doi.org/10.26650/EurJBiol.2019.0009



TIMELINE


Submitted17.05.2019
Accepted24.07.2019
Published Online06.12.2019

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.