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DOI :10.26650/EurJBiol.2024.1442720   IUP :10.26650/EurJBiol.2024.1442720    Full Text (PDF)

Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.)

Chudamani Sharma PrakashYi Hong Wang

Objective: The Solanaceae family includes many unique and popular fruits and vegetables such as potato, tomato, and pepper. Peppers are a group of plants that produce pungent fruits favoured by many in various parts of the world. This spiciness is due to a class of compounds called capsaicinoid which are synthesized in peppers but not in tomatoes. Both pepper and tomato genomes have been sequenced, and genes involved in the capsaicinoid biosynthesis pathway have been identified in both genomes. Along with expression profiling, there were only three genes in the tomato pathway that were not expressed. In this study, we attempted to overexpress the three pepper genes in tomato to produce spicy fruits.

Materials and Methods: The three genes, BCAT (branched-chain amino acid aminotransferase), Kas (ketoacyl-ACP synthase), and CS/AT (capsaicin synthase/acyltransferase), were separated using P2Am and T2Am sequences in a tricistronic cassette driven by the 35S promoter. Transgenic tomato plants containing the gene construct were generated via Agrobacterium-mediated transformation.

Results: RT-PCR indicated that the genes were expressed in all transgenic tomato plants. Some transgenic fruits resembled hot peppers with elongated shapes and wrinkled surfaces, but tomato fruits were not spicy based on two-person tasting evaluations.

Conclusion: P2Am and T2Am sequences can be used for the overexpression of multiple genes in tomatoes. Further studies with tissue-specific promoters and metabolic profiling are necessary.


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APA

Prakash, C.S., & Wang, Y.H. (2024). Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.). European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2024.1442720


AMA

Prakash C S, Wang Y H. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.). European Journal of Biology. 2024;0(0):-. https://doi.org/10.26650/EurJBiol.2024.1442720


ABNT

Prakash, C.S.; Wang, Y.H. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.). European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2024.


Chicago: Author-Date Style

Prakash, Chudamani Sharma, and Yi Hong Wang. 2024. “Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.).” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2024.1442720


Chicago: Humanities Style

Prakash, Chudamani Sharma, and Yi Hong Wang. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.).” European Journal of Biology 0, no. 0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1442720


Harvard: Australian Style

Prakash, CS & Wang, YH 2024, 'Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.)', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2024.1442720


Harvard: Author-Date Style

Prakash, C.S. and Wang, Y.H. (2024) ‘Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.)’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2024.1442720 (22 Nov. 2024).


MLA

Prakash, Chudamani Sharma, and Yi Hong Wang. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.).” European Journal of Biology, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1442720


Vancouver

Prakash CS, Wang YH. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.). European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2024.1442720 doi: 10.26650/EurJBiol.2024.1442720


ISNAD

Prakash, ChudamaniSharma - Wang, YiHong. Overexpression of Pepper Capsaicinoid Pathway Genes in Tomato (Solanum lycopersicum L.)”. European Journal of Biology 0/0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1442720



TIMELINE


Submitted25.02.2024
Accepted08.05.2024
Published Online01.07.2024

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