European Journal of Biology

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DOI :10.5152/EurJBiol.2017.1705   IUP :10.5152/EurJBiol.2017.1705    Full Text (PDF)

Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos

Ünsal Veli Üstündağ, Ismail Unal, Perihan Seda Ateş Kalkan, Ahmet Ata Alturfan, Türkan Yigitbasi, Ebru Emekli Alturfan

Di-(2-ethylhexyl)-phthalate (DEHP) is used in polyethylene terephthalate plastics and bisphenol-A (BPA) is used to make polycarbonate plastics. The health-concerning effects of DEHP and BPA indicate their potential toxic effects. The Wnt/βcatenin signaling pathway is necessary for embryonic development. The c-MYC proto-oncogene (MYC) has been identified as a Wnt/β-catenin target gene, and MYC is a transcription factor that regulates the expression of many gene products related to cell proliferation, growth, differentiation, and apoptosis. Activation and overexpression of the MYC gene has been reported in many human cancers and during tumorigenesis. The aim of this study was to investigate the relationship between oxidantantioxidant balance and c-myc in BPA- and DEHP-exposed zebrafish embryos. The real-time-polymerase chain reaction was used to determine c-myc expression. Lipid peroxidation (LPO), nitric oxide (NO) levels, and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities were determined in homogenates prepared from pooled embryo samples. Increased expression of c-myc and decreased GST activity were observed in the BPA and DEHP groups. NO levels increased and SOD activity decreased in the BPA group, whereas LPO increased in the DEHP group. Disruption of oxidant-antioxidant balance in DEHP- and BPA-exposed zebrafish embryos was associated with increased c-myc expression and may be an important mechanism for the toxic effects of these chemicals.


Keywords: Di(2-ethylhexyl) phthalate, bisphenol A, oxidant-antioxidant balance, c-myc, zebrafish embryo

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APA

Üstündağ, Ü.V., Unal, I., Ateş Kalkan, P.S., Alturfan, A.A., Yigitbasi, T., & Emekli Alturfan, E. (2017). Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos. European Journal of Biology, 76(1), 26-30. https://doi.org/10.5152/EurJBiol.2017.1705


AMA

Üstündağ Ü V, Unal I, Ateş Kalkan P S, Alturfan A A, Yigitbasi T, Emekli Alturfan E. Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos. European Journal of Biology. 2017;76(1):26-30. https://doi.org/10.5152/EurJBiol.2017.1705


ABNT

Üstündağ, Ü.V.; Unal, I.; Ateş Kalkan, P.S.; Alturfan, A.A.; Yigitbasi, T.; Emekli Alturfan, E. Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos. European Journal of Biology, [Publisher Location], v. 76, n. 1, p. 26-30, 2017.


Chicago: Author-Date Style

Üstündağ, Ünsal Veli, and Ismail Unal and Perihan Seda Ateş Kalkan and Ahmet Ata Alturfan and Türkan Yigitbasi and Ebru Emekli Alturfan. 2017. “Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos.” European Journal of Biology 76, no. 1: 26-30. https://doi.org/10.5152/EurJBiol.2017.1705


Chicago: Humanities Style

Üstündağ, Ünsal Veli, and Ismail Unal and Perihan Seda Ateş Kalkan and Ahmet Ata Alturfan and Türkan Yigitbasi and Ebru Emekli Alturfan. “Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos.” European Journal of Biology 76, no. 1 (May. 2025): 26-30. https://doi.org/10.5152/EurJBiol.2017.1705


Harvard: Australian Style

Üstündağ, ÜV & Unal, I & Ateş Kalkan, PS & Alturfan, AA & Yigitbasi, T & Emekli Alturfan, E 2017, 'Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos', European Journal of Biology, vol. 76, no. 1, pp. 26-30, viewed 23 May. 2025, https://doi.org/10.5152/EurJBiol.2017.1705


Harvard: Author-Date Style

Üstündağ, Ü.V. and Unal, I. and Ateş Kalkan, P.S. and Alturfan, A.A. and Yigitbasi, T. and Emekli Alturfan, E. (2017) ‘Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos’, European Journal of Biology, 76(1), pp. 26-30. https://doi.org/10.5152/EurJBiol.2017.1705 (23 May. 2025).


MLA

Üstündağ, Ünsal Veli, and Ismail Unal and Perihan Seda Ateş Kalkan and Ahmet Ata Alturfan and Türkan Yigitbasi and Ebru Emekli Alturfan. “Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos.” European Journal of Biology, vol. 76, no. 1, 2017, pp. 26-30. [Database Container], https://doi.org/10.5152/EurJBiol.2017.1705


Vancouver

Üstündağ ÜV, Unal I, Ateş Kalkan PS, Alturfan AA, Yigitbasi T, Emekli Alturfan E. Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos. European Journal of Biology [Internet]. 23 May. 2025 [cited 23 May. 2025];76(1):26-30. Available from: https://doi.org/10.5152/EurJBiol.2017.1705 doi: 10.5152/EurJBiol.2017.1705


ISNAD

Üstündağ, ÜnsalVeli - Unal, Ismail - Ateş Kalkan, PerihanSeda - Alturfan, AhmetAta - Yigitbasi, Türkan - Emekli Alturfan, Ebru. “Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos”. European Journal of Biology 76/1 (May. 2025): 26-30. https://doi.org/10.5152/EurJBiol.2017.1705



TIMELINE


Submitted16.10.2017
Accepted23.10.2017

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