European Journal of Biology

SUBMITINFORMATION
EnglishTürkçe

Research Article


DOI :10.26650/EurJBiol.2023.1216574   IUP :10.26650/EurJBiol.2023.1216574    Full Text (PDF)

Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid

Esmail Nabizadeh, Narges Dolatmand, Masoud Haghshenas, Khadijeh Ahmadi

Objective: Heat stress is a significant factor leading to decreased crop yield. Exceeding the plant’s temperature tolerance threshold in ecosystems often results in significant cellular damage and potentially cellular death. Signaling elicitors may mitigate elevated temperatures’ detrimental impact and enhance plant defense mechanisms.

Materials and Methods: The present study investigates the influence of varying temperatures (25, 30, 35, 40, and 45°C) and pre-harvest salicylic acid (SA) application (0, 0.5, 1.5, 2.5, 5, and 10 mM) on the morpho-physiological and biochemical attributes of maize. A factorial-based experiment was set up following a completely randomized design and conducted in a growth room.

Results: The findings demonstrated that a 2.5 mM SA treatment at 35°C produced the largest plant leaf area and total chlorophyll content. The temperature and SA application interplay on carotenoid content were maximum at 5 mM. SA treatment under hightemperature conditions effectively elevated proline content, chl a, chl b, chl total, and malondialdehyde compared to untreated plants. The peak stomatal conductance was also observed with a 2.5 mM SA treatment at 30°C. The maximal catalase and peroxidase activities were recorded at 35°C. Furthermore, 2.5 mM SA at 25°C resulted in the highest levels of soluble proteins and RWC. SA (2.5 mM) applied at 30°C was more efficient at decreasing H2O2 production. The highest proline content was observed with 2.5 mM SA at 45°C.

Conclusion: SA(2.5 mM) treatment can have optimal effects on maize plant growth parameters under high-temperature conditions, potentially mitigating the damaging effects of heat stress.

Keywords: Antioxidant, Biological Yield, Heat Stress, Proline, Soluble Protein.

PDF View

References

  • Xu N, York K, Miller P, Cheikh N. Co-regulation of ear growth and internode elongation in corn. Plant Growth Reg. 2004;44:231-241. google scholar
  • Atkinson NJ, Urwin PE. The interaction of plant biotic and abiotic stresses: From genes to the field. JExp Bot. 2012;63:3523-3543. google scholar
  • Impa SM, Perumal R, Bean SR, John Sunoj VS, Krishna Ja-gadish SV. Water deficit and heat stress induced alterations in grain physico-chemical characteristics and micronutrient composition in field grown grain sorghum. J Cer Sci Technol. 2019;86:124-131. google scholar
  • Crafts-Brandner SJ, Salvucci ME. Sensitivity of photosyn-thesis in a C4 plant, maize, to heat stress. Plant Physiol. 2002;129:1773-1780. google scholar
  • Ma M, Wang P, Yang R. UV-B mediates isoflavone accumulation and oxidative-an 460 tioxidant system responses in germinating soybean. Food Chem. 2019;275:628-636. google scholar
  • Wang LJ, Fan L, Loescher W, et al. Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biol. 2010;10:1-10. doi:10.1186/1471-2229-10-34. google scholar
  • Sajjad Y, Jaskani MJ, Ashraf MY, et al. Response of morpho-logical and physiological growth attributes to foliar applica-tion of plant growth regulators in gladiolus ’white prosperity’. Pak J Agric Sci. 2014;51:123-129. google scholar
  • Pere/ MGF, Rocha-Gu/man NE, Mercado-Silva E, et al. Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food Chem. 2014;156:273-278. google scholar
  • Karlidag H, Yildirim E, Turan M. Exogenous applications of sal-icylic acid affect quality and yield of strawberry grown under antifrost heated greenhouse conditions. J Plant Nutr Soil Sci. 2009;172:270-276 google scholar
  • Hayat S, Ali B, Ahmad A. Salicylic acid: Biosynthesis, metabolism and physiological role in plants, In: Salicylic Acid: A Plant Hormone. Springer. 2007; pp:1-14. google scholar
  • Dat JF, Lopez-Delgado H, Foyer CH, Scott IM. Effects of salicylic acid on oxidative stress and thermotolerance in tobacco. J Plant Physiol. 2000;156:659-665. google scholar
  • Souri MK, Tohidloo G. Effectiveness of different methods of salicylic acid application on growth characteristics of tomato seedlings under salinity. Chem Biol Technol Agric. 2019;6:26. doi:10.1186/s40538-019-0169-9. google scholar
  • Singh B, Usha K. Salicylic acid induced physiological and bio-chemical changes in wheat seedlings under water stress. Plant Growth Regul. 2003;39:137-141. google scholar
  • Lichtenthaler HK, Wellburn AR. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in differ-ent solvents. Biochem Soc Trans. 1983;11:591-592. google scholar
  • Lutts S, Kinet JM, Bouharmont J. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity re-sistance. Ann Bot. 1996;78:389-398. google scholar
  • Ritchie SW, Nguyen HT, Scott Holaday A. Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci. 1990;30:105-111. google scholar
  • Kang HM, Saltveit ME. Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiol Plant. 2002;115:571-576. google scholar
  • Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-126. google scholar
  • Upadhyaya A, Sankhla D, Davis TD, et al. Effect of paclobu-trazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. J Plant Physiol. 1985;121:453-461. google scholar
  • Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22:867-880. google scholar
  • Irigoyen JJ, Einerich DW, Saııclıe/-I.)ıa/ M. Water stress in-duced changes in concentrations of proline and total soluble sug-ars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant. 1992;84:55-60. google scholar
  • Bradford M. A Rapid and sensitive method for the quantifica-tion of microgram quantities of protein utili/ing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254. google scholar
  • Heath RL, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Bioph. 1968;125:189-198. google scholar
  • Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Sci. 2000;15:59-66. google scholar
  • Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39:205. doi:10.1007/BF00018060. google scholar
  • Salvucci ME. Association of Rubisco activase with chaperonin-60p: A possible mechanism for protecting photosynthesis during heat stress. JExp Bot. 2008;59:1923-1933. google scholar
  • Gupta R. Manganese repairs the oxygen-evolving complex (OEC) in mai/e (Zea mays L.) damage during seawater vulnerability. J Plant Nutr Soil Sci. 2020;20:1387-1396. google scholar
  • Yashavanthakumar KJ, Baviskar VS, Navathe S, et al. Impact of heat and drought stress on phenological development and yield in bread wheat. Plant Physiol Rep. 2021;26:357-367. google scholar
  • Kalaji HM, Schansker G, Brestic M. Frequently asked ques-tions about chlorophyll fluorescence, the sequel. Photosynth Res. 2017;132:13-66. google scholar
  • Ali F, Waters DLE, Ovenden B, et al. Heat stress during grain fill reduces head rice yield through genotype-dependent increased husk biomass and grain breakage. J Cereal Sci. 2019;90:102820.doi:10.1016/j.jcs.2019.102820. google scholar
  • Wahid A, Gelani S, Ashraf M, Foolad MR. Heat tolerance in plants: An overview. Environ Exp Bot. 2007;61:199-223. google scholar
  • Portis AR, Parry MAJ. Discoveries in Rubisco (Ribulose 1, 5-bisphosphate carboxylase/oxygenase): A historical perspective. Photosynth Res. 2007;94:121-143. google scholar
  • Khan W, Prithiviraj B, Smith DL. Photosynthetic responses of corn and soybean to foliar application of salicylates. J Plant Physiol. 2003;160:485-492. google scholar
  • Beney L, Gervais P. Influence of the fluidity of the membrane on the response of microorganisms to environmental stresses. Appl Microbiol Bio. 2001;57:34-42. google scholar
  • Allakhverdiev SI, Kreslavski VD, Klimov VV, et al. Heat stress: An overview of molecular responses in photosynthesis. Photosynth Res. 2008;98(1-3):541-550. google scholar
  • Reddy RA, Kumar B, Reddy PS, et al. Molecular cloning and characterization of genes encoding Pennisetum glaucum ascor-bate peroxidase and heat-shock factor: interlinking oxidative and heat-stress responses. J Plant Physiol. 2009;166:1646-1659. google scholar
  • Karwa S, Arya SS, Maurya S, Pal M. Physiological characteriza-tion of reproductive stage heat stress tolerance in contrasting rice genotypes. Plant Physiol Rep. 2020;25:157-162. google scholar
  • Taiz L, Zeiger E. Plant physiology 5th Ed. Sunderland: Sinauer Associates. 2010;pp:464. google scholar
  • Morimoto RI. Proteotoxic stress and inducible chaperone net-works in neurodegenerative disease and aging. Genes Dev. 2008;22:1427-1438. google scholar
  • Borsani O, Valpuesta V, Botella MA. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol. 2001;126:1024-1030. google scholar
  • Gharib FA. Effect of salicylic acid on the growth, metabolic ac-tivities and oil content of basil and marjoram. Inter. J Agric Biol. 2006;4:485-492. google scholar
  • Ashraf M, Foolad MR. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot. 2007;59:206-216. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Nabizadeh, E., Dolatmand, N., Haghshenas, M., & Ahmadi, K. (2023). Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid. European Journal of Biology, 82(2), 167-178. https://doi.org/10.26650/EurJBiol.2023.1216574


AMA

Nabizadeh E, Dolatmand N, Haghshenas M, Ahmadi K. Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid. European Journal of Biology. 2023;82(2):167-178. https://doi.org/10.26650/EurJBiol.2023.1216574


ABNT

Nabizadeh, E.; Dolatmand, N.; Haghshenas, M.; Ahmadi, K. Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 167-178, 2023.


Chicago: Author-Date Style

Nabizadeh, Esmail, and Narges Dolatmand and Masoud Haghshenas and Khadijeh Ahmadi. 2023. “Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid.” European Journal of Biology 82, no. 2: 167-178. https://doi.org/10.26650/EurJBiol.2023.1216574


Chicago: Humanities Style

Nabizadeh, Esmail, and Narges Dolatmand and Masoud Haghshenas and Khadijeh Ahmadi. “Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid.” European Journal of Biology 82, no. 2 (Dec. 2024): 167-178. https://doi.org/10.26650/EurJBiol.2023.1216574


Harvard: Australian Style

Nabizadeh, E & Dolatmand, N & Haghshenas, M & Ahmadi, K 2023, 'Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid', European Journal of Biology, vol. 82, no. 2, pp. 167-178, viewed 27 Dec. 2024, https://doi.org/10.26650/EurJBiol.2023.1216574


Harvard: Author-Date Style

Nabizadeh, E. and Dolatmand, N. and Haghshenas, M. and Ahmadi, K. (2023) ‘Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid’, European Journal of Biology, 82(2), pp. 167-178. https://doi.org/10.26650/EurJBiol.2023.1216574 (27 Dec. 2024).


MLA

Nabizadeh, Esmail, and Narges Dolatmand and Masoud Haghshenas and Khadijeh Ahmadi. “Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 167-178. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1216574


Vancouver

Nabizadeh E, Dolatmand N, Haghshenas M, Ahmadi K. Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid. European Journal of Biology [Internet]. 27 Dec. 2024 [cited 27 Dec. 2024];82(2):167-178. Available from: https://doi.org/10.26650/EurJBiol.2023.1216574 doi: 10.26650/EurJBiol.2023.1216574


ISNAD

Nabizadeh, Esmail - Dolatmand, Narges - Haghshenas, Masoud - Ahmadi, Khadijeh. “Physiological and Biochemical Changes of Maize (Zea mays ‘MV500’) in Response to Heat Stress under Levels of Salicylic Acid”. European Journal of Biology 82/2 (Dec. 2024): 167-178. https://doi.org/10.26650/EurJBiol.2023.1216574



TIMELINE


Submitted09.12.2022
Accepted09.11.2023
Published Online07.12.2023

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.