European Journal of Biology

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DOI :10.26650/EurJBiol.2024.1550119   IUP :10.26650/EurJBiol.2024.1550119    Full Text (PDF)

Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats

Dilek Özbeyli, Ali Şen, Naziye Özkan Yena, Ayşe Nur Hazar Yavuz, Deniz Mukaddes Türet, Meral Yüksel, Leyla Bitiş, Ahmet Doğan, Aslı Aykaç

Objective: This study evaluated the antioxidant and anti-inflammatory effects of Sideritis perfoliata (SP) ethanolic extract in vitro and its impact on anti-inflammatory, antiapoptotic, oxidative stress, and tissue damage-related parameters in an ulcerative colitis (UC) rat model.

Materials and Methods: Dried and ground aerial parts of SP were extracted with 90% ethanol using maceration. Then, the SP extract’s radical scavenging, anti-5-lipoxygenase (LOX) activities, and total secondary metabolic quantities were evaluated in vitro. In in vivo experiments, following acetic acid (AA)-induced colitis, physiological saline (PS) (1 mL, og), sulfasalazine (SS) (100 mg/kg bwt, 1mL, og), and SP extract (200 mg/kg bwt, 1mL, og) treatments were administered to AA, SS, and SP groups, respectively, once daily for three consecutive days. PS was given to the control group. At the 72nd hour, the rats were euthanized. Analyses were performed on colon tissues using ELISA, Chemiluminescence assay, Haematoxylin and Eosin staining.

Results: SP extract exhibited good antioxidant activity against 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and strong anti-5-LOX activity. The total amounts of phenol, flavonoid, and triterpene compounds in the extract were determined. Compared with the AA group, SS or SP extract treatments reduced tumour necrosis factor-α, interleukin-1β, interleukin-17, tool-like receptor-9, matrix metalloproteinases-3, caspase-9, caspase-3, luminol, and lucigenin chemiluminescence levels, macroscopic and microscopic morphological scores in colon.

Conclusion: Due to its flavonoids, phenolic acids, triterpene content, anti-5-LOX, and free radical scavenging activities, SP extract attenuates proinflammatory cytokines, mitochondrial apoptotic caspases, and oxidative stress. Our findings support the ethnopharmacological use of SP for colitis.

Keywords: Sideritis perfoliata, Ulcerative colitis, Cytokines, Apoptosis, Oxidative stress

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APA

Özbeyli, D., Şen, A., Özkan Yena, N., Hazar Yavuz, A.N., Türet, D.M., Yüksel, M., Bitiş, L., Doğan, A., & Aykaç, A. (2024). Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats. European Journal of Biology, 83(2), 222-231. https://doi.org/10.26650/EurJBiol.2024.1550119


AMA

Özbeyli D, Şen A, Özkan Yena N, Hazar Yavuz A N, Türet D M, Yüksel M, Bitiş L, Doğan A, Aykaç A. Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats. European Journal of Biology. 2024;83(2):222-231. https://doi.org/10.26650/EurJBiol.2024.1550119


ABNT

Özbeyli, D.; Şen, A.; Özkan Yena, N.; Hazar Yavuz, A.N.; Türet, D.M.; Yüksel, M.; Bitiş, L.; Doğan, A.; Aykaç, A. Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats. European Journal of Biology, [Publisher Location], v. 83, n. 2, p. 222-231, 2024.


Chicago: Author-Date Style

Özbeyli, Dilek, and Ali Şen and Naziye Özkan Yena and Ayşe Nur Hazar Yavuz and Deniz Mukaddes Türet and Meral Yüksel and Leyla Bitiş and Ahmet Doğan and Aslı Aykaç. 2024. “Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats.” European Journal of Biology 83, no. 2: 222-231. https://doi.org/10.26650/EurJBiol.2024.1550119


Chicago: Humanities Style

Özbeyli, Dilek, and Ali Şen and Naziye Özkan Yena and Ayşe Nur Hazar Yavuz and Deniz Mukaddes Türet and Meral Yüksel and Leyla Bitiş and Ahmet Doğan and Aslı Aykaç. “Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats.” European Journal of Biology 83, no. 2 (Dec. 2024): 222-231. https://doi.org/10.26650/EurJBiol.2024.1550119


Harvard: Australian Style

Özbeyli, D & Şen, A & Özkan Yena, N & Hazar Yavuz, AN & Türet, DM & Yüksel, M & Bitiş, L & Doğan, A & Aykaç, A 2024, 'Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats', European Journal of Biology, vol. 83, no. 2, pp. 222-231, viewed 22 Dec. 2024, https://doi.org/10.26650/EurJBiol.2024.1550119


Harvard: Author-Date Style

Özbeyli, D. and Şen, A. and Özkan Yena, N. and Hazar Yavuz, A.N. and Türet, D.M. and Yüksel, M. and Bitiş, L. and Doğan, A. and Aykaç, A. (2024) ‘Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats’, European Journal of Biology, 83(2), pp. 222-231. https://doi.org/10.26650/EurJBiol.2024.1550119 (22 Dec. 2024).


MLA

Özbeyli, Dilek, and Ali Şen and Naziye Özkan Yena and Ayşe Nur Hazar Yavuz and Deniz Mukaddes Türet and Meral Yüksel and Leyla Bitiş and Ahmet Doğan and Aslı Aykaç. “Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats.” European Journal of Biology, vol. 83, no. 2, 2024, pp. 222-231. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1550119


Vancouver

Özbeyli D, Şen A, Özkan Yena N, Hazar Yavuz AN, Türet DM, Yüksel M, Bitiş L, Doğan A, Aykaç A. Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats. European Journal of Biology [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];83(2):222-231. Available from: https://doi.org/10.26650/EurJBiol.2024.1550119 doi: 10.26650/EurJBiol.2024.1550119


ISNAD

Özbeyli, Dilek - Şen, Ali - Özkan Yena, Naziye - Hazar Yavuz, AyşeNur - Türet, DenizMukaddes - Yüksel, Meral - Bitiş, Leyla - Doğan, Ahmet - Aykaç, Aslı. “Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats”. European Journal of Biology 83/2 (Dec. 2024): 222-231. https://doi.org/10.26650/EurJBiol.2024.1550119



TIMELINE


Submitted14.09.2024
Accepted12.11.2024
Published Online19.12.2024

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