European Journal of Biology

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Research Article


DOI :10.26650/EurJBiol.2023.1247199   IUP :10.26650/EurJBiol.2023.1247199    Full Text (PDF)

Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells

Burak Durmaz, Latife Merve Oktay Çelebi, Hikmet Memmedov, Nur Selvi Günel, Hatice Kalkan Yıldırım, Eser Yıldırım Sözmen

Objective: Propolis has been found to have various effects, including antioxidant and anti-inflammatory properties, according to studies. In this recent research, we discovered that reducing allergenic compounds in propolis through biotransformation using specific Lactobacillus plantarum strains enhanced its anti-inflammatory qualities. The study aimed to identify the extraction methods and solvents that had the most significant anti-inflammatory effects and assess how L. plantarum strains biotransformation of propolis affected these qualities in THP-1 cell line cultures.

Materials and Methods: Propolis samples were biotransformed with different concentrations (1.5%, 2.5%, 3.5%) of several L. plantarum strains (ISLG-2, ATCC®8014, visbyvac) before extraction using various solvents (ethanol, polyethylene glycol-PEG, water) and ultrasound treatments (300 W/40 Hz for 5, 10, 15 min). Liquid chromatography-mass spectrometer/mass spectrometry was used for phenolic analysis of the samples. ELISA test kits were employed to assess NF-kβ, IL-1α, IL-1β, IL-6, IL-10, TNF-α, IFN-γ , COX-1 in the cell culture supernatant.

Results: Results showed that, except for NF-kβ, all cytokine levels decreased in four separate propolis samples. Caffeic acid, kaempferol, ferulic acid, quercetin, pelargonin, and naringenin were the key physiologically active components associated with the anti-inflammatory activity of propolis. The biotransformation process to reduce allergen compounds did not alter propolis’s anti-inflammatory properties.

Conclusion: In samples that were dissolved in water, dissolved in ethanol+biotransformed with L. plantarum ATCC®8014, dissolved in water+biotransformed with L. plantarum ATCC®8014, and dissolved in water+sonicated for 15 min and biotransformed with L. plantarum ATCC®8014, the maximum anti-inflammatory effect of propolis was assessed.

Keywords: LPS, inflammation, propolis, biotransformation, extraction

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APA

Durmaz, B., Oktay Çelebi, L.M., Memmedov, H., Günel, N.S., Kalkan Yıldırım, H., & Sözmen, E.Y. (2023). Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells. European Journal of Biology, 82(2), 132-141. https://doi.org/10.26650/EurJBiol.2023.1247199


AMA

Durmaz B, Oktay Çelebi L M, Memmedov H, Günel N S, Kalkan Yıldırım H, Sözmen E Y. Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells. European Journal of Biology. 2023;82(2):132-141. https://doi.org/10.26650/EurJBiol.2023.1247199


ABNT

Durmaz, B.; Oktay Çelebi, L.M.; Memmedov, H.; Günel, N.S.; Kalkan Yıldırım, H.; Sözmen, E.Y. Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 132-141, 2023.


Chicago: Author-Date Style

Durmaz, Burak, and Latife Merve Oktay Çelebi and Hikmet Memmedov and Nur Selvi Günel and Hatice Kalkan Yıldırım and Eser Yıldırım Sözmen. 2023. “Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells.” European Journal of Biology 82, no. 2: 132-141. https://doi.org/10.26650/EurJBiol.2023.1247199


Chicago: Humanities Style

Durmaz, Burak, and Latife Merve Oktay Çelebi and Hikmet Memmedov and Nur Selvi Günel and Hatice Kalkan Yıldırım and Eser Yıldırım Sözmen. “Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells.” European Journal of Biology 82, no. 2 (Dec. 2024): 132-141. https://doi.org/10.26650/EurJBiol.2023.1247199


Harvard: Australian Style

Durmaz, B & Oktay Çelebi, LM & Memmedov, H & Günel, NS & Kalkan Yıldırım, H & Sözmen, EY 2023, 'Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells', European Journal of Biology, vol. 82, no. 2, pp. 132-141, viewed 26 Dec. 2024, https://doi.org/10.26650/EurJBiol.2023.1247199


Harvard: Author-Date Style

Durmaz, B. and Oktay Çelebi, L.M. and Memmedov, H. and Günel, N.S. and Kalkan Yıldırım, H. and Sözmen, E.Y. (2023) ‘Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells’, European Journal of Biology, 82(2), pp. 132-141. https://doi.org/10.26650/EurJBiol.2023.1247199 (26 Dec. 2024).


MLA

Durmaz, Burak, and Latife Merve Oktay Çelebi and Hikmet Memmedov and Nur Selvi Günel and Hatice Kalkan Yıldırım and Eser Yıldırım Sözmen. “Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 132-141. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1247199


Vancouver

Durmaz B, Oktay Çelebi LM, Memmedov H, Günel NS, Kalkan Yıldırım H, Sözmen EY. Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells. European Journal of Biology [Internet]. 26 Dec. 2024 [cited 26 Dec. 2024];82(2):132-141. Available from: https://doi.org/10.26650/EurJBiol.2023.1247199 doi: 10.26650/EurJBiol.2023.1247199


ISNAD

Durmaz, Burak - Oktay Çelebi, LatifeMerve - Memmedov, Hikmet - Günel, NurSelvi - Kalkan Yıldırım, Hatice - Sözmen, EserYıldırım. “Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells”. European Journal of Biology 82/2 (Dec. 2024): 132-141. https://doi.org/10.26650/EurJBiol.2023.1247199



TIMELINE


Submitted03.02.2023
Accepted08.08.2023
Published Online15.09.2023

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