European Journal of Biology

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Research Article


DOI :10.26650/EurJBiol.2023.1301847   IUP :10.26650/EurJBiol.2023.1301847    Full Text (PDF)

Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish

Esra Dandin, İsmail Ünal, Merih Beler, Ünsal Veli Üstündağ, Derya Cansız, Perihan Seda Ateş Kalkan, Ebru Emekli Alturfan

Objective: An excessive buildup of adipose tissue is a defining feature of overnutrition, and a significant fraction of the world’s population suffers from obesity. Overnutrition is associated with the deterioration of mitochondrial functions in the brain in the case of obesity. In this study, we evaluated the effects of stevioside (ST) which is a calorie-free, naturally occurring herbal sweetener made from Stevia rebaudiana (Bertoni) on the oxidant-antioxidant balance in the brain in cases of overnutrition. Accordingly, the effects of ST consumption on the oxidant-antioxidant balance in the brain was evaluated and determined in a case study of overfeeding adult zebrafish for 15 days.

Materials and Methods: Zebrafish were placed in four groups; the control group (C); overfed group (OF); low-dose (1mg/L) ST treated OF group (OF+LDS); and the high-dose ST (5mg/L) treated OF group (OF+HDS). The levels of lipid peroxidation (LPO) were evaluated together with nitric oxide (NO) to determine the oxidant status. The antioxidant status from the activities of the superoxide dismutase (SOD) and glutathione S-transferase (GST) were determined in brain tissues.

Results: The ST treatment decreased the increased LPO and NO levels in overfed zebrafish and increased SOD and GST activities in a dose-dependent manner.

Conclusion: ST exerted an antioxidant effect on the possible damage mechanisms that could occur in the brain in case of overnutrition by decreasing oxidative stress and improving the antioxidant enzyme activities. 

Keywords: Stevioside, overfeeding, brain, oxidant-antioxidant status, zebrafish

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APA

Dandin, E., Ünal, İ., Beler, M., Üstündağ, Ü.V., Cansız, D., Ateş Kalkan, P.S., & Emekli Alturfan, E. (2023). Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish. European Journal of Biology, 82(2), 258-262. https://doi.org/10.26650/EurJBiol.2023.1301847


AMA

Dandin E, Ünal İ, Beler M, Üstündağ Ü V, Cansız D, Ateş Kalkan P S, Emekli Alturfan E. Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish. European Journal of Biology. 2023;82(2):258-262. https://doi.org/10.26650/EurJBiol.2023.1301847


ABNT

Dandin, E.; Ünal, İ.; Beler, M.; Üstündağ, Ü.V.; Cansız, D.; Ateş Kalkan, P.S.; Emekli Alturfan, E. Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 258-262, 2023.


Chicago: Author-Date Style

Dandin, Esra, and İsmail Ünal and Merih Beler and Ünsal Veli Üstündağ and Derya Cansız and Perihan Seda Ateş Kalkan and Ebru Emekli Alturfan. 2023. “Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish.” European Journal of Biology 82, no. 2: 258-262. https://doi.org/10.26650/EurJBiol.2023.1301847


Chicago: Humanities Style

Dandin, Esra, and İsmail Ünal and Merih Beler and Ünsal Veli Üstündağ and Derya Cansız and Perihan Seda Ateş Kalkan and Ebru Emekli Alturfan. “Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish.” European Journal of Biology 82, no. 2 (May. 2024): 258-262. https://doi.org/10.26650/EurJBiol.2023.1301847


Harvard: Australian Style

Dandin, E & Ünal, İ & Beler, M & Üstündağ, ÜV & Cansız, D & Ateş Kalkan, PS & Emekli Alturfan, E 2023, 'Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish', European Journal of Biology, vol. 82, no. 2, pp. 258-262, viewed 1 May. 2024, https://doi.org/10.26650/EurJBiol.2023.1301847


Harvard: Author-Date Style

Dandin, E. and Ünal, İ. and Beler, M. and Üstündağ, Ü.V. and Cansız, D. and Ateş Kalkan, P.S. and Emekli Alturfan, E. (2023) ‘Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish’, European Journal of Biology, 82(2), pp. 258-262. https://doi.org/10.26650/EurJBiol.2023.1301847 (1 May. 2024).


MLA

Dandin, Esra, and İsmail Ünal and Merih Beler and Ünsal Veli Üstündağ and Derya Cansız and Perihan Seda Ateş Kalkan and Ebru Emekli Alturfan. “Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 258-262. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1301847


Vancouver

Dandin E, Ünal İ, Beler M, Üstündağ ÜV, Cansız D, Ateş Kalkan PS, Emekli Alturfan E. Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish. European Journal of Biology [Internet]. 1 May. 2024 [cited 1 May. 2024];82(2):258-262. Available from: https://doi.org/10.26650/EurJBiol.2023.1301847 doi: 10.26650/EurJBiol.2023.1301847


ISNAD

Dandin, Esra - Ünal, İsmail - Beler, Merih - Üstündağ, ÜnsalVeli - Cansız, Derya - Ateş Kalkan, PerihanSeda - Emekli Alturfan, Ebru. “Stevioside Improves Brain Oxidant-Antioxidant Status in Overfed Zebrafish”. European Journal of Biology 82/2 (May. 2024): 258-262. https://doi.org/10.26650/EurJBiol.2023.1301847



TIMELINE


Submitted24.05.2023
Accepted07.07.2023
Published Online13.09.2023

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