The Role of rs4626 and rs7221352 Polymorphisms on  the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients

Başoğlu Köseahmet Fulya; Eker Candan; Öztürk Musa; Özdemir Şebnem; Köksal Ayhan; Baybaş Savim; Günel Tuba

doi:https://dx.doi.org/10.26650/EurJBiol.2022.1191215

Research Article

DOI :10.26650/EurJBiol.2022.1191215 IUP :10.26650/EurJBiol.2022.1191215 Full Text (PDF)

The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients

Fulya Başoğlu Köseahmet, Candan Eker, Musa Öztürk, Şebnem Özdemir, Ayhan Köksal, Savim Baybaş, Tuba Günel

Objective: Multiple sclerosis often causes neurological disability and reduced quality of life. Genetic biomarkers are important tools for the diagnosis and prognoses of diseases. This study has been conducted to explore the haplotype frequencies formed by rs4626 and rs7221352 single-nucleotide polymorphisms (SNPs) in the coding region variant (rs4626) and 5' upstream region intron variant (rs7221352) of the transducer of the ERBB2.1 (TOB1) gene in individuals with relapsingremitting multiple sclerosis.

Materials and Methods: Thirty patients with an Expanded Disability Status Scale (EDSS) score<3, 30 patients with EDSS≥5, and 30 healthy controls participated in the study. The TOB1 rs4626 T/C and rs7221352 G/A single-base variations were applied using the quantitative real-time polymerase chain reaction method in accordance with the TaqMan SNP Genotyping Assays instructions.

Results: The genotype frequencies of TOB1 rs4626 TT/TC/CC were respectively 3.3%, 53.3%, and 43.3% in the EDSS<3 cases and 10%, 53.3%, and 36.7% in the EDSS≥5 cases. The genotype frequencies of TOB1 rs7221352 GG/AG/AA were respectively 3.3%, 86.7%, and 10% in the EDSS<3 cases and 10%, 70%, and 20% in the EDSS≥5 cases. With respect to the estimated values in the study cohort, allelic variant frequency was higher in the patient group for both SNP variants (p<0.001).

Conclusion: The presence of variant alleles in the rs4626 and rs7221352 polymorphisms in TOB1 may have a role in the disease immunopathogenesis. Further investigations involving larger groups are required to understand the effects of TOB1.

Keywords: TOB1, multiple sclerosis, single-nucleotide polymorphisms, allelic variation, quantitative real-time PCR

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APA

Başoğlu Köseahmet, F., Eker, C., Öztürk, M., Özdemir, Ş., Köksal, A., Baybaş, S., & Günel, T. (2022). The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients. European Journal of Biology, 81(2), 197-205. https://doi.org/10.26650/EurJBiol.2022.1191215

AMA

Başoğlu Köseahmet F, Eker C, Öztürk M, Özdemir Ş, Köksal A, Baybaş S, Günel T. The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients. European Journal of Biology. 2022;81(2):197-205. https://doi.org/10.26650/EurJBiol.2022.1191215

ABNT

Başoğlu Köseahmet, F.; Eker, C.; Öztürk, M.; Özdemir, Ş.; Köksal, A.; Baybaş, S.; Günel, T. The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients. European Journal of Biology, [Publisher Location], v. 81, n. 2, p. 197-205, 2022.

Chicago: Author-Date Style

Başoğlu Köseahmet, Fulya, and Candan Eker and Musa Öztürk and Şebnem Özdemir and Ayhan Köksal and Savim Baybaş and Tuba Günel. 2022. “The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients.” European Journal of Biology 81, no. 2: 197-205. https://doi.org/10.26650/EurJBiol.2022.1191215

Chicago: Humanities Style

Başoğlu Köseahmet, Fulya, and Candan Eker and Musa Öztürk and Şebnem Özdemir and Ayhan Köksal and Savim Baybaş and Tuba Günel. “The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients.” European Journal of Biology 81, no. 2 (Nov. 2024): 197-205. https://doi.org/10.26650/EurJBiol.2022.1191215

Harvard: Australian Style

Başoğlu Köseahmet, F & Eker, C & Öztürk, M & Özdemir, Ş & Köksal, A & Baybaş, S & Günel, T 2022, 'The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients', European Journal of Biology, vol. 81, no. 2, pp. 197-205, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2022.1191215

Harvard: Author-Date Style

Başoğlu Köseahmet, F. and Eker, C. and Öztürk, M. and Özdemir, Ş. and Köksal, A. and Baybaş, S. and Günel, T. (2022) ‘The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients’, European Journal of Biology, 81(2), pp. 197-205. https://doi.org/10.26650/EurJBiol.2022.1191215 (22 Nov. 2024).

MLA

Başoğlu Köseahmet, Fulya, and Candan Eker and Musa Öztürk and Şebnem Özdemir and Ayhan Köksal and Savim Baybaş and Tuba Günel. “The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients.” European Journal of Biology, vol. 81, no. 2, 2022, pp. 197-205. [Database Container], https://doi.org/10.26650/EurJBiol.2022.1191215

Vancouver

Başoğlu Köseahmet F, Eker C, Öztürk M, Özdemir Ş, Köksal A, Baybaş S, Günel T. The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients. European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];81(2):197-205. Available from: https://doi.org/10.26650/EurJBiol.2022.1191215 doi: 10.26650/EurJBiol.2022.1191215

ISNAD

Başoğlu Köseahmet, Fulya - Eker, Candan - Öztürk, Musa - Özdemir, Şebnem - Köksal, Ayhan - Baybaş, Savim - Günel, Tuba. “The Role of rs4626 and rs7221352 Polymorphisms on the TOB1 Gene in Turkish Relapsing-Remitting Multiple Sclerosis Patients”. European Journal of Biology 81/2 (Nov. 2024): 197-205. https://doi.org/10.26650/EurJBiol.2022.1191215

Volume 81, Issue 22022, P. 197-205

TIMELINE

Submitted	18.10.2022
Accepted	13.12.2022
Published Online	30.12.2022

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