European Journal of Biology

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Research Article


DOI :10.26650/EurJBiol.2023.1274707   IUP :10.26650/EurJBiol.2023.1274707    Full Text (PDF)

Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells

Ömer Erdoğan, Burçin İrem Abas, Özge Çevik

Objective: Nuclear factor kappa B (NF-κB) is one pathway that controls the expression of genes involved in many cancer events such as proliferation, apoptosis, metastasis, and invasion. Usnic acid is a molecule with many biological effects such as being anticholinergic, gastroprotective, anti-inflammatory, anti-cancerous, and especially antioxidant. This study aims to mechanistically examine the apoptotic behaviors of usnic acid in DU145 prostate cancer cells and the molecules it acts on in the NF-κB pathway.

Materials and Methods: This study investigates the apoptotic changes in DU145 cells after usnic acid administration through JC-1 staining and caspase-3 activity measurements. In addition, it tests the effects of usnic acid on subunit p50 and p65 protein and gene expressions in the NF-κB pathway through the respective Western blot and qPCR measurements.

Results: The IC50 values of usnic acid at 24 and 48 h in DU145 cells were calculated as 167.06±12.35 μM and 42.15±3.76 μM, respectively. In addition, JC-1 staining showed usnic acid-treated DU145 cells to trigger apoptosis by increasing the membrane permeability of their mitochondria. NF-κB p50 protein expression was also found to be suppressed after usnic acid administration.

Conclusion: The results of this study show usnic acid administration to suppress proliferation and to induce mitochondrial apoptosis by suppressing the NF-κB pathway in DU145 cells. This effect of usnic acid indicates it to be combinable with chemotherapeutic agents and evaluable as an alternative in cancer treatment. 

Keywords: Apoptosis, NF-κB pathway, prostate cancer, usnic acid

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APA

Erdoğan, Ö., Abas, B.İ., & Çevik, Ö. (2023). Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells. European Journal of Biology, 82(2), 251-257. https://doi.org/10.26650/EurJBiol.2023.1274707


AMA

Erdoğan Ö, Abas B İ, Çevik Ö. Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells. European Journal of Biology. 2023;82(2):251-257. https://doi.org/10.26650/EurJBiol.2023.1274707


ABNT

Erdoğan, Ö.; Abas, B.İ.; Çevik, Ö. Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells. European Journal of Biology, [Publisher Location], v. 82, n. 2, p. 251-257, 2023.


Chicago: Author-Date Style

Erdoğan, Ömer, and Burçin İrem Abas and Özge Çevik. 2023. “Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells.” European Journal of Biology 82, no. 2: 251-257. https://doi.org/10.26650/EurJBiol.2023.1274707


Chicago: Humanities Style

Erdoğan, Ömer, and Burçin İrem Abas and Özge Çevik. “Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells.” European Journal of Biology 82, no. 2 (Dec. 2024): 251-257. https://doi.org/10.26650/EurJBiol.2023.1274707


Harvard: Australian Style

Erdoğan, Ö & Abas, Bİ & Çevik, Ö 2023, 'Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells', European Journal of Biology, vol. 82, no. 2, pp. 251-257, viewed 27 Dec. 2024, https://doi.org/10.26650/EurJBiol.2023.1274707


Harvard: Author-Date Style

Erdoğan, Ö. and Abas, B.İ. and Çevik, Ö. (2023) ‘Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells’, European Journal of Biology, 82(2), pp. 251-257. https://doi.org/10.26650/EurJBiol.2023.1274707 (27 Dec. 2024).


MLA

Erdoğan, Ömer, and Burçin İrem Abas and Özge Çevik. “Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells.” European Journal of Biology, vol. 82, no. 2, 2023, pp. 251-257. [Database Container], https://doi.org/10.26650/EurJBiol.2023.1274707


Vancouver

Erdoğan Ö, Abas Bİ, Çevik Ö. Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells. European Journal of Biology [Internet]. 27 Dec. 2024 [cited 27 Dec. 2024];82(2):251-257. Available from: https://doi.org/10.26650/EurJBiol.2023.1274707 doi: 10.26650/EurJBiol.2023.1274707


ISNAD

Erdoğan, Ömer - Abas, Burçinİrem - Çevik, Özge. “Usnic Acid Exerts Antiproliferative and Apoptotic Effects by Suppressing NF-B p50 in DU145 Cells”. European Journal of Biology 82/2 (Dec. 2024): 251-257. https://doi.org/10.26650/EurJBiol.2023.1274707



TIMELINE


Submitted31.03.2023
Accepted20.06.2023
Published Online13.09.2023

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