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DOI :10.26650/EurJBiol.2024.1442790   IUP :10.26650/EurJBiol.2024.1442790    Tam Metin (PDF)

Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression

Azadeh HekmatElaheh GhanbariSaeed Hesami Tackallou

Objective: Currently, treating triple-negative breast cancer (TNBC) poses a significant challenge. Eryngium billardierei is widely employed as a medicinal plant, particularly for the treatment of cancers. However, there is a notable shortage of research investigating the effects of E. billardierei on TNBC. This study seeks to evaluate the anticancer capabilities of the ethanolic extract of E. billardierei concerning TNBC, as well as its impact on the expression of CDKN2A, MDM2, and HER2.

Materials and Methods: The MTT assay, N-acetyl-L-cysteine (NAC) assay, AO/EtBr dual staining, and flow cytometry were utilized to assess the anticancer potential of the extract of E. billardierei against theMDA-MB-231 cell line. In addition, the impact of E. billardierei extract on the expression of CDKN2A, MDM2, and HER2 was examined using real-time PCR.

Results: The results conclusively demonstrated that the extract of E. billardierei exhibited a dose-dependent suppression of the growth of MDA-MB-231 cells. Moreover, treatment with the extract induced apoptosis in cancer cells. The inhibitory effect on growth was attributed to the generation of ROS, leading to a significant upregulation (P<0.01) of CDKN2A expression and a downregulation (P<0.05) of MDM2 expression. Conversely, the expression of HER2 remained unaffected following extract treatment.

Conclusion: The outcomes of this study provide a foundation for considering the use of E. billardierei extract in the treatment of TNBC. However, additional research is warranted to investigate the mechanisms of E. billardierei extract across a broader spectrum of TNBC cell lines.


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Referanslar

  • 1. Subramaniyan V, Fuloria S, Gupta G, et al. A review on epidermal growth factor receptor’s role in breast and nonsmall cell lung cancer. Chem Biol Interact. 2022;351:109735. doi:10.1016/j.cbi.2021.109735 google scholar
  • 2. Hekmat A, Saso L, Lather V, Pandita D, Kostova I, Saboury AA. Recent advances in nanomaterials of group XIV elements of periodic table in breast cancer treatment. Pharmaceutics. 2022:14(12):2640. doi: 10.3390/pharmaceutics14122640 google scholar
  • 3. Zhao R, Choi BY, Lee M-H, Bode AM, Dong Z. Implications of genetic and epigenetic alterations of CDKN2A (p16INK4a) in cancer. E Bio Medicine. 2016;8:30-39. google scholar
  • 4. Al-Saran N, Subash-Babu P, Al-Nouri DM, Alfawaz HA, Al-shatwi AA. Zinc enhances CDKN2A, pRb1 expression and regulates functional apoptosis via upregulation of p53 and p21 expression in human breast cancer MCF-7 cell. Environ Toxicol Pharmacol. 2016;47:19-27. google scholar
  • 5. Hou H, Sun D, Zhang X. The role of MDM2 amplification and overexpression in therapeutic resistance of malignant tumors. Cancer Cell Int. 2019;19(1):216. doi:10.1186/s12935-019-0937-4 google scholar
  • 6. McGrowder DA, Miller FG, Nwokocha CR, et al. Medicinal herbs used in traditional management of breast cancer: Mechanisms of action. Medicines. 2020;7(8):47. doi:10.3390/medicines7080047 google scholar
  • 7. Roshanravan N, Asgharian P, Dariushnejad H, et al. Eryngium billardieri induces apoptosis via bax gene expression in pancreatic cancer cells. Adv Pharm Bull. 2018;8(4):667-674. google scholar
  • 8. Daneshzadeh MS, Abbaspour H, Amjad L, Nafchi AM. An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri F. Delaroche. J Food Meas Charact. 2020;14:708-715. google scholar
  • 9. Esmaeili S, Irani M, Moazzeni Zehan H, Keramatian B, Tavakoli Harandi Z, Hamzeloo-Moghadam M. Cytotoxic activity of some ethnic medicinal plants from southwest of Iran. J Pharmacognosy Res. 2016;3:43-47. google scholar
  • 10. Hasanbeiglu S, Hosseini K, Molavi O, Asgharian P, Tarhriz V. Eryngium billardieri extract and fractions induce apoptosis in cancerous cells. Anticancer Agents Med Chem. 2022;22(11):2189-2201. google scholar
  • 11. Forouhandeh H, Rezaei Param Z, Molavi O, Asgharian P, Tarhriz V. Evaluation of anti-proliferative activity of Eryngium caucasicum on melanoma cancer cells. BMC Complement Med Ther. 2022; 22(1):134. doi:10.1186/s12906-022-03618-w google scholar
  • 12. Afshari M, Malayeri AR, Mohammadshahi M. Effects of Eryngium caucasicum extract on testosterone, inflammation and oxidative status of nicotinamide-streptozotocin induced type-2 diabetes in male rats. J Contemp Med Sci. 2019;5(2):77-81. google scholar
  • 13. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008;3(6):1101-1108. google scholar
  • 14. Jarzçbski M, Siejak P, Smulek W, et al. Plant extracts containing saponins affects the stability and biological activity of hempseed oil emulsion system. Molecules. 2020;25(11):2696. doi:10.3390/molecules25112696 google scholar
  • 15. Robbins RJ. Phenolic acids in foods: An overview of analytical methodology. J Agric Food Chem. 2003;51(10):2866-2887. google scholar
  • 16. Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020;12(1):1-12. google scholar
  • 17. Hosseini K, Ariya MP, Molavi O, Asgharian P, Tarhriz V. The fractions of Eryngium thyrsoideum extract sensitize breast cancer cells to apoptosis. J Nat Pharm Prod. 2022;17(3):118888. doi:10.5812/jjnpp.118888 google scholar
  • 18. Nordin FJ, Pearanpan L, Chan KM, et al. Immunomodulatory potential of Clinacanthus nutans extracts in the coculture of triple-negative breast cancer cells, MDA-MB-231, and THP-1 macrophages. PLoS One. 2021;16(8):e0256012. doi:10.1371/journal.pone.0256012 google scholar
  • 19. Alshaeri HK, Alasmari MM, Natto ZS, Pino-Figueroa A. Effects of Annona muricata extract on triple-negative breast cancer cells mediated through EGFR signaling. Cancer Manag Res. 2020;12:12519-12526. google scholar
  • 20. Nguyen ST, Nguyen HT-L, Truong KD. Comparative cytotoxic effects of methanol, ethanol and DMSO on human cancer cell lines. Biomed Res Ther. 2020;7(7):3855-3859. google scholar
  • 21. Samson J, Derlipanska M, Zaheed O, Dean K. Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010. BMC Cancer. 2021;21(1):1-20. google scholar
  • 22. Hekmat A, Rabizadeh M, Safavi M, Hajebrahimi Z. The comparison of the apoptosis effects of titanium dioxide nanoparticles into MDA-MB-231 cell line in microgravity and gravity conditions. Nanomed J. 2019;6(2):120-127. google scholar
  • 23. Pagayeva L, Kongül E, Geylan R, Karatoprak G§, Tugay O, editors. Analysis of the cytotoxic effects of Eryngium billardieri Delar. Extracts on MCF7 Cell Line. Proceedings. 2017;1:1055. doi:10.3390/proceedings1101055 google scholar
  • 24. Khan MA, Siddiqui S, Ahmad I, et al. Phytochemicals from Ajwa dates pulp extract induce apoptosis in human triplenegative breast cancer by inhibiting AKT/mTOR pathway and modulating Bcl-2 family proteins. Sci Rep. 2021;11(1):10322. doi:10.1038/s41598-021-89420-z google scholar
  • 25. Zahedian S, Hekmat A, Tackallou SH, Ghoranneviss M. The impacts of prepared plasma-activated medium (PAM) combined with doxorubicin on the viability of MCF-7 breast cancer cells: a new cancer treatment strategy. Rep Biochem Mol Biol. 2022;10(4):640-652. google scholar
  • 26. Carpenter RL, Lo H-W. Regulation of apoptosis by HER2 in breast cancer. J Carcinog Mutagen Res. 2013;2013:1-13. doi:10.4172/2157-2518.S7-003 google scholar
  • 27. Vousden KH, Prives C. Blinded by the light: the growing complexity of p53. Cell. 2009;137(3):413-431. google scholar
  • 28. Yavari S, Hekmat A, Saedari S. The ethanolic extract of Eryngium billardierei F. Delaroche restrains protein glycation in human serum albumin: An in vitro study. J Serb Chem Soc. 2023, November, doi:10.2298/JSC230725091Y google scholar
  • 29. Kopustinskiene DM, Jakstas V, Savickas A, Bernatoniene J. Flavonoids as anticancer agents. Nutrients. 2020;12(2):457. doi:10.3390/nu12020457 google scholar
  • 30. Khan AU, Dagur HS, Khan M, Malik N, Alam M, Mushtaque M. Therapeutic role of flavonoids and in cancer prevention: Current trends and future perspectives. Eur J Med Chem Rep. 2021;3:100010. doi:10.1016/j.ejmcr.2021.100010 google scholar
  • 31. Fabiani R. Anti-cancer properties of olive oil secoiridoid phenols: A systematic review of in vivo studies. Food Funct. 2016;7(10):4145-4159. google scholar

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APA

Hekmat, A., Ghanbari, E., & Tackallou, S.H. (2024). Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2024.1442790


AMA

Hekmat A, Ghanbari E, Tackallou S H. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression. European Journal of Biology. 2024;0(0):-. https://doi.org/10.26650/EurJBiol.2024.1442790


ABNT

Hekmat, A.; Ghanbari, E.; Tackallou, S.H. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2024.


Chicago: Author-Date Style

Hekmat, Azadeh, and Elaheh Ghanbari and Saeed Hesami Tackallou. 2024. “Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2024.1442790


Chicago: Humanities Style

Hekmat, Azadeh, and Elaheh Ghanbari and Saeed Hesami Tackallou. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression.” European Journal of Biology 0, no. 0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1442790


Harvard: Australian Style

Hekmat, A & Ghanbari, E & Tackallou, SH 2024, 'Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2024.1442790


Harvard: Author-Date Style

Hekmat, A. and Ghanbari, E. and Tackallou, S.H. (2024) ‘Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2024.1442790 (22 Nov. 2024).


MLA

Hekmat, Azadeh, and Elaheh Ghanbari and Saeed Hesami Tackallou. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression.” European Journal of Biology, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1442790


Vancouver

Hekmat A, Ghanbari E, Tackallou SH. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression. European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2024.1442790 doi: 10.26650/EurJBiol.2024.1442790


ISNAD

Hekmat, Azadeh - Ghanbari, Elaheh - Tackallou, SaeedHesami. Effect of the Ethanolic Extract of Eryngium billardieri on the Viability of the Triple-Negative Breast Cancer Cell Line (MDA-MB-231) and CDKN2A, MDM2, and HER2 Expression”. European Journal of Biology 0/0 (Nov. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1442790



ZAMAN ÇİZELGESİ


Gönderim25.02.2024
Kabul10.05.2024
Çevrimiçi Yayınlanma02.07.2024

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