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DOI :10.26650/EurJBiol.2024.1353842   IUP :10.26650/EurJBiol.2024.1353842    Tam Metin (PDF)

Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells

Gamze Nur ÖterEzgi DurmuşAli ŞenAbdürrahim Koçyiğit

Objective: Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment. This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line.

Materials and Methods: A549 cells were treated with RTE and RTC individually. The MTT assay was used to quantitatively detect RTE and RTC’s cytotoxic effects. The fluorescent signal indicator H2DCF-DA was used to detect cellular reactive oxygen species (ROS) production. Apoptosis was evaluated by fluorescence microscope after acridine orange/ethidium bromide fluorescent staining, annexin V-FITC and immunoblotting analyses, immunofluorescence, and imaging.

Results: Both RTE and RTC induced cytotoxicity in A549 cells in a dose-dependently, which was accompanied with induced ROS accumulation. Both early and late apoptotic cells detected by flow cytometry were increased in the RTE- and RTC-treated cells. In addition, the results show RTC to have higher cytotoxic and apoptotic effects and increased ROS-generation capacity than RTE. Therefore, the polarity of the solvent used to exert the anticancer effect of R. tereticaulis leaves is crucial.

Conclusion: This is the first anti-cancer activity study on R. tereticaulis. The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment. 


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Referanslar

  • Hirsch FR, Scagliotti GV, Mulshine JL, et al. Lung can-cer: current therapies and new targeted treatments. Lancet. 2017;389(10066):299-311. google scholar
  • Oser MG, Niederst MJ, Sequist LV, Engelman JA. Transformation from non-small-cell lung cancer to small-cell lung cancer: Molec-ular drivers and cells of origin. Lancet Oncol. 2015;16(4):e165-e172. doi:10.1016/S1470-2045(14)71180-5 google scholar
  • Zappa C, Mousa, SA. Non-small cell lung cancer: Cur-rent treatment and future advances. Transl Lung Cancer Res. 2016;5(3):288. doi:10.21037/tlcr.2016.06.07 google scholar
  • Li N, Mai Y, Liu Q, Gou G, Yang J. Docetaxel-loaded D-a-tocopheryl polyethylene glycol-1000 succinate liposomes im-prove lung cancer chemotherapy and reverse multidrug resistance. Drug Deliv Transl Res. 2021;11:131-141. google scholar
  • Lynch TJ, Kass F, Elias AD, et al. Cisplatin, 5-fluorouracil, and etoposide for advanced non-small cell lung cancer. Cancer. 1993;71(10):2953-2957. google scholar
  • Zhang M, Li M, Du L, Zeng J, Yao T, Jin Y. Paclitaxel-in-liposome-in-bacteria for inhalation treatment of primary lung cancer. Inter J Pharm. 2020;578:119177. doi: 10.1016/j.ijpharm.2020.119177 google scholar
  • Storz P. Mitochondrial ROS-radical detoxification, mediated by protein kinase D. Trends Cell Bio. 2007;17(1):13-18. google scholar
  • Prakash O, Kumar A, Kumar P, Ajeet. Anticancer potential of plants and natural products. Am J Pharmacol Sci. 2013;1(6):104-115. google scholar
  • Hyun TK, Kim HC, Ko YJ, Kim JS. Antioxidant, a-glucosidase inhibitory and anti-inflammatory effects of aerial parts extract from Korean crowberry (Empetrum nigrum var. japonicum). Saudi J Bio Sci. 2016;23(2):181-188. google scholar
  • George BP, Abrahamse H. Traditional uses and bioactivities of common Rubus species with reference to cancer: A mini-review. Phyto:Pot Ther Appl. 2021;259-270. google scholar
  • Tuzlacı E. Türkiye Bitkileri Geleneksel İlaç Rehberi (Turkey’s Plants Traditional Medicine Guide), First ed. İstanbul: İstanbul Tıp Kitabevleri Press; 2016. google scholar
  • Gudej J, Tomczyk M. Determination of flavonoids, tannins and ellagic acid in leaves from Rubus L. species. Arch Pharm Res. 2004;(27):1114-1119. google scholar
  • Wolniak M, Tomczykowa M, Tomczyk M, Gudej J, Wawer I. An-tioxidant activity of extracts and flavonoids from Bidens tripartita. Acta Poloniae Pharm. 2007; 64(5): 441-447. google scholar
  • Sen A, Ozbeyli D, Teralı K, et al. Protective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A com-bined in silico, in vitro and in vivo study. Chemico-Bio Inter. 2023;369:110263. doi:10.1016/j.cbi.2022.110263 google scholar
  • Aksoy H, Demirbag C, Sen A, et al. Evaluation of biochemical parameters in Rubus tereticaulis treated rats and its implications in wound healing. Mol Cell Biochem. 2020; 472:67-78. google scholar
  • Kocyigit A, Guler EM, Karatas E, Caglar H, Bulut H. Dose-dependent proliferative and cytotoxic effects of melatonin on hu-man epidermoid carcinoma and normal skin fibroblast cells. Mut Res Genet Toxico Envir Mutag. 2018;829:50-60. google scholar
  • Wu T, Qiang L, Chen FH, et al. LFG-500, a newly synthe-sized flavonoid, induced a reactive oxygen species-mitochondria-mediated apoptosis in hepatocarcinoma cells. Bio Preven Nutr. 2011;1(2):132-138. google scholar
  • Mcgahon AJ, Martin SJ, Bissonnette RP, et al. The end of the (cell) line: Methods for the study of apoptosis in vitro. Method Cell Biol. 1995;46:153-185. google scholar
  • Wen T, Song L, Hua S. Perspectives and controversies regarding the use of natural products for the treatment of lung cancer. Cancer Med. 2021;10(7):2396-2422. google scholar
  • Zhao X, Song J, Lee JH, Kim SY, Park KY. Antioxidation and cancer cell (HT-29) antiproliferation effects of Rubus coreanus Miquel bamboo salt. J Cancer Prev. 2010; 15(4):306-312. google scholar
  • Kim EJ, Lee YJ, Shin HK, Park JHY. Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. Nutrition. 2005;21(11-12):1141-1148. google scholar
  • Saikolappan S, Kumar B, Shishodia G, Koul S, Koul HK. Reactive oxygen species and cancer: A complex interaction. Cancer Lett. 2019;452:132-143. google scholar
  • Simon HU, Haj-Yehia A, Levi-Schaffer F. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 2000;5:415-418. google scholar
  • Kocyigit A, Koyuncu I, Taskin A, Dikilitas M, Bahadori F, Turkkan B. Antigenotoxic and antioxidant potentials of newly derivatized compound naringenin-oxime relative to naringenin on human mononuclear cells. Drug Chem Toxicol. 2016;39(1):66-73. google scholar
  • George BP, Abrahamse H. Increased oxidative stress induced by rubus bioactive compounds induce apoptotic cell death in hu-man breast cancer cells. Oxid Med Cell Longev. 2019;6797921. doi:10.1155/2019/6797921 google scholar
  • Lavrik IN, Golks A, Krammer PH. Caspases: Pharmacological manipulation of cell death. J Clinic Inves. 2005;115(10):2665-2672. google scholar
  • Galluzzi L, Maiuri MC, Vitale I, et al. Cell death modalities: Classification and pathophysiological implications. Cell Death Differ. 2007;14(7):1237-1243. google scholar
  • Plackal Adimuriyil George B, Tynga IM, Abrahamse H. In vitro antiproliferative effect of the acetone extract of Rubus fairholmianus gard. Root on human colorectal cancer cells. BioMed Res Inter. 2015;2015:165037. doi:10.1155/2015/165037 google scholar
  • Erel SB, Demir S, Nalbantsoy A, et al. Bioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoa. Pharm Biol. 2014;52(6):775-781. google scholar
  • Coussens LM, Werb Z. Inflammation and cancer. Nature. 2002;420(6917):860-867. google scholar
  • Morrıson WB. Inflammation and cancer: A comparative view. J Vet Int Med. 2012; 26(1):18-31. google scholar
  • Rayburn ER, Ezell SJ, Zhang R. Anti-inflammatory agents for cancer therapy. Mol Cell Pharm. 2009;1(1):29-43. google scholar
  • Kawamori T, Lubet R, Steele VE, et al. Chemopreventive effect of curcumin, a naturally occurring anti-inflammatory agent, during the promotion/progression stages of colon cancer. Cancer Res. 1999;59(3):597-601. google scholar
  • Muller AG, Sarker SD, Saleem IY, Hutcheon GA. Delivery of natural phenolic compounds for the potential treatment of lung cancer. DARU J Pharma Sci. 2019;27: 433-449. google scholar
  • Amararathna M, Johnston MR, Rupasinghe HPV. Plant polyphe-nols as chemopreventive agents for lung cancer. Inter J Mol Sci. 2016;17(8):1352. doi:10.3390/ijms17081352 google scholar
  • Murakami A, Ashida H, Terao J. Multitargeted cancer prevention by quercetin. Cancer Lett. 2008;269(2):315-325. google scholar

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APA

Öter, G.N., Durmuş, E., Şen, A., & Koçyiğit, A. (2024). Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, 83(1), 50-59. https://doi.org/10.26650/EurJBiol.2024.1353842


AMA

Öter G N, Durmuş E, Şen A, Koçyiğit A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology. 2024;83(1):50-59. https://doi.org/10.26650/EurJBiol.2024.1353842


ABNT

Öter, G.N.; Durmuş, E.; Şen, A.; Koçyiğit, A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, [Publisher Location], v. 83, n. 1, p. 50-59, 2024.


Chicago: Author-Date Style

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. 2024. “Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology 83, no. 1: 50-59. https://doi.org/10.26650/EurJBiol.2024.1353842


Chicago: Humanities Style

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology 83, no. 1 (Nov. 2024): 50-59. https://doi.org/10.26650/EurJBiol.2024.1353842


Harvard: Australian Style

Öter, GN & Durmuş, E & Şen, A & Koçyiğit, A 2024, 'Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells', European Journal of Biology, vol. 83, no. 1, pp. 50-59, viewed 8 Nov. 2024, https://doi.org/10.26650/EurJBiol.2024.1353842


Harvard: Author-Date Style

Öter, G.N. and Durmuş, E. and Şen, A. and Koçyiğit, A. (2024) ‘Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells’, European Journal of Biology, 83(1), pp. 50-59. https://doi.org/10.26650/EurJBiol.2024.1353842 (8 Nov. 2024).


MLA

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology, vol. 83, no. 1, 2024, pp. 50-59. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1353842


Vancouver

Öter GN, Durmuş E, Şen A, Koçyiğit A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology [Internet]. 8 Nov. 2024 [cited 8 Nov. 2024];83(1):50-59. Available from: https://doi.org/10.26650/EurJBiol.2024.1353842 doi: 10.26650/EurJBiol.2024.1353842


ISNAD

Öter, GamzeNur - Durmuş, Ezgi - Şen, Ali - Koçyiğit, Abdürrahim. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells”. European Journal of Biology 83/1 (Nov. 2024): 50-59. https://doi.org/10.26650/EurJBiol.2024.1353842



ZAMAN ÇİZELGESİ


Gönderim01.09.2023
Kabul01.03.2024
Çevrimiçi Yayınlanma02.04.2024

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