Research Article


DOI :10.26650/EurJBiol.2024.1353842   IUP :10.26650/EurJBiol.2024.1353842    Full Text (PDF)

Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells

Gamze Nur ÖterEzgi DurmuşAli ŞenAbdürrahim Koçyiğit

Objective:Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment. This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line.

Materials and Methods:A549 cells were treated with RTE and RTC individually. The MTT assay was used to quantitatively detect RTE and RTC’s cytotoxic effects. The fluorescent signal indicator H2DCF-DA was used to detect cellular reactive oxygen species (ROS) production. Apoptosis was evaluated by fluorescence microscope after acridine orange/ethidium bromide fluorescent staining, annexin V-FITC and immunoblotting analyses, immunofluorescence, and imaging.

Results:Both RTE and RTC induced cytotoxicity in A549 cells in a dose-dependently, which was accompanied with induced ROS accumulation. Both early and late apoptotic cells detected by flow cytometry were increased in the RTE- and RTC-treated cells. In addition, the results show RTC to have higher cytotoxic and apoptotic effects and increased ROS-generation capacity than RTE. Therefore, the polarity of the solvent used to exert the anticancer effect of R. tereticaulis leaves is crucial.

Conclusion:This is the first anti-cancer activity study on R. tereticaulis. The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment. 


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APA

Öter, G.N., Durmuş, E., Şen, A., & Koçyiğit, A. (2019). Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2024.1353842


AMA

Öter G N, Durmuş E, Şen A, Koçyiğit A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology. 2019;0(0):-. https://doi.org/10.26650/EurJBiol.2024.1353842


ABNT

Öter, G.N.; Durmuş, E.; Şen, A.; Koçyiğit, A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2019.


Chicago: Author-Date Style

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. 2019. “Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2024.1353842


Chicago: Humanities Style

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology 0, no. 0 (May. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1353842


Harvard: Australian Style

Öter, GN & Durmuş, E & Şen, A & Koçyiğit, A 2019, 'Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 3 May. 2024, https://doi.org/10.26650/EurJBiol.2024.1353842


Harvard: Author-Date Style

Öter, G.N. and Durmuş, E. and Şen, A. and Koçyiğit, A. (2019) ‘Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2024.1353842 (3 May. 2024).


MLA

Öter, Gamze Nur, and Ezgi Durmuş and Ali Şen and Abdürrahim Koçyiğit. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells.” European Journal of Biology, vol. 0, no. 0, 2019, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1353842


Vancouver

Öter GN, Durmuş E, Şen A, Koçyiğit A. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology [Internet]. 3 May. 2024 [cited 3 May. 2024];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2024.1353842 doi: 10.26650/EurJBiol.2024.1353842


ISNAD

Öter, GamzeNur - Durmuş, Ezgi - Şen, Ali - Koçyiğit, Abdürrahim. Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells”. European Journal of Biology 0/0 (May. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1353842



TIMELINE


Submitted01.09.2023
Accepted01.03.2024
Published Online02.04.2024

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